Gao, Min; Wang, Rui; Yu, Fabiao; Youc, Jinmao; Chen, Lingxin
发表期刊JOURNAL OF MATERIALS CHEMISTRY B
ISSN2050-750X
2018-05-07
卷号6期号:17页码:2608-2619
关键词HYDROGEN POLYSULFIDESOXIDATIVE STRESSIN-VIVOCYSTEINE PERSULFIDESBIOLOGICAL-SYSTEMSCHEMICAL PROBESCANCER-CELLSSULFIDECONSTRUCTIONSUPEROXIDE
研究领域Materials Science
DOI10.1039/c7tb03200e
产权排序[Gao, Min; Wang, Rui; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Gao, Min] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yu, Fabiao; Youc, Jinmao; Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Key Lab Life Organ Anal, Qufu 273165, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要Ischemia is a pathological condition owing to the deficiency of blood supply to a limited area of tissue. Ischemia can induce burst production of reactive oxygen species and lead to oxidative damage. As a family member of reactive sulfur species, sulfane sulfur plays important physiological roles in many biological events including synthesis of cofactors, modulation of enzyme activities, sulfuration of tRNA, and especially regulation of the intracellular redox state. We hypothesize that the endogenous level of sulfane sulfur will be adjusted to deal with ischemia-induced oxidative damage. Therefore, the bioimaging of sulfane sulfur real-time changes during ischemia is important for better understanding its physiological processes. Herein, we describe the development of a mitochondria-targeted fluorescent probe Mito-SH that allowed for selective and sensitive detection of sulfane sulfur. Mito-SH is designed on the basis of the tautomerization of sulfane sulfur to thiosulfoxide, which ensures its high selectivity and sensitivity. A lipophilic triphenylphosphonium cation is selected as the mitochondria-targeted moiety, which can precisely navigate Mito-SH into mitochondria. The emission profile of azo-BODIPY fluorophore locates at the near-infrared region, which deeply penetrates tissue and effectively avoids the interference of biological background. Mito-SH exhibits the desirable combination of selectivity, sensitivity and excellent fluorescence response upon reaction with sulfane sulfur in cells. By employing Mito-SH, we evaluate the real-time sulfane sulfur dynamic changes under oxygen-glucose deprivation. Finally, Mito-SH has been successfully used for imaging sulfane sulfur changes caused by acute ischemia in mice.
文章类型Article
资助机构National Nature Science Foundation of China [21575159, 21775162, 41776110, 21405172]; program of Youth Innovation Promotion Association, CAS [2015170]; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22]; Instrument Developing Project of the CAS [YZ201662]; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology [SATM201705]
收录类别SCI
语种英语
关键词[WOS]HYDROGEN POLYSULFIDES; OXIDATIVE STRESS; IN-VIVO; CYSTEINE PERSULFIDES; BIOLOGICAL-SYSTEMS; CHEMICAL PROBES; CANCER-CELLS; SULFIDE; CONSTRUCTION; SUPEROXIDE
研究领域[WOS]Materials Science, Biomaterials
WOS记录号WOS:000431421800013
引用统计被引频次：22[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24465
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
3.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Key Lab Life Organ Anal, Qufu 273165, Peoples R China
推荐引用方式
GB/T 7714Gao, Min,Wang, Rui,Yu, Fabiao,et al. Imaging and evaluation of sulfane sulfur in acute brain ischemia using a mitochondria-targeted near-infrared fluorescent probe[J]. JOURNAL OF MATERIALS CHEMISTRY B,2018,6(17):2608-2619.
APAGao, Min,Wang, Rui,Yu, Fabiao,Youc, Jinmao,&Chen, Lingxin.(2018).Imaging and evaluation of sulfane sulfur in acute brain ischemia using a mitochondria-targeted near-infrared fluorescent probe.JOURNAL OF MATERIALS CHEMISTRY B,6(17),2608-2619.
MLAGao, Min,et al."Imaging and evaluation of sulfane sulfur in acute brain ischemia using a mitochondria-targeted near-infrared fluorescent probe".JOURNAL OF MATERIALS CHEMISTRY B 6.17(2018):2608-2619.
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