Han, Xiaoyue; Wang, Rui; Song, Xinyu; Yu, Fabiao; Chen, Lingxin
发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
2018-07-03
卷号90期号:13页码:8108-8115
关键词PROTEIN-KINASE PATHWAYSHUMAN HEALTHSELENIUMSELENOLSCELLSSELENOPROTEINSSPECTROMETRYDYSFUNCTIONTISSUESPLASMA
研究领域Chemistry
DOI10.1021/acs.analchem.8b01306
产权排序[Han, Xiaoyue; Wang, Rui; Song, Xinyu; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Han, Xiaoyue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Lingxin] Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要As important active sites of oxidoreductase in mitochondria, selenocysteine (Sec) takes the responsibility for cytoprotective effect and intracellular redox homeostasis. Carbon disulfide (CS2) is a common solvent in industry, which can inhibit the activities of oxidoreductase and induce oxidative stress. It is necessary to investigate the cytoprotective effect of Sec against CS2 exposure. After integrated, the response moiety 2,4-dinitrobenzenesulfonamide and mitochondrial targeting moiety into the near-infrared heptamethine cyanine fluorophore, we develop a mitochondrial targeting near-infrared ratiometric fluorescent probe Mito-diNO(2) for the selective and sensitive analysis of Sec concentration fluctuations in living cells and in mice models under the stimulation of CS2. The probe can effectively accumulate in mitochondria and selectively detect the endogenous Sec concentrations in BRL 3A, RH-35, HL-7702, HepG2, and SMMC-7721 cell lines. The results indicate that CS2 exposure can lead to a decrease of Sec level and result in mitochondrial related acute inflammation. The exogenous supplement of Sec can protect cells from oxidative damage and reduce the symptoms of inflammation. We also establish CS2 induced acute and chronic hepatitis mice models to examine the tissue toxicity of CS2 and cytoprotection of Sec in liver. The organism can increase the concentration of Sec to deal with the damage caused by CS2 in acute hepatitis mice model. Also the exogenous supplement of Sec for the two mice models can effectively defend the CS2 induced liver damage. The real-time imaging of Sec concentrations in liver can be used to assess the degrees of liver injury during CS2 poisoning. The above applications make our probe a potential candidate for the clinical accurate diagnosis and treatment of CS2 poisoning.
文章类型Article
资助机构National Nature Science Foundation of China [21775162, 21575159, 41776110, 21405172]; program of Youth Innovation Promotion Association, CAS [2015170]; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22]; Instrument Developing Project of the Chinese Academy of Sciences [YZ201662]
收录类别SCI
语种英语
关键词[WOS]PROTEIN-KINASE PATHWAYS; HUMAN HEALTH; SELENIUM; SELENOLS; CELLS; SELENOPROTEINS; SPECTROMETRY; DYSFUNCTION; TISSUES; PLASMA
研究领域[WOS]Chemistry, Analytical
WOS记录号WOS:000438008600045
引用统计被引频次：24[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24439
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
3.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China
推荐引用方式
GB/T 7714Han, Xiaoyue,Wang, Rui,Song, Xinyu,et al. Evaluation Selenocysteine Protective Effect in Carbon Disulfide Induced Hepatitis with a Mitochondrial Targeting Ratiometric Near-Infrared Fluorescent Probe[J]. ANALYTICAL CHEMISTRY,2018,90(13):8108-8115.
APAHan, Xiaoyue,Wang, Rui,Song, Xinyu,Yu, Fabiao,&Chen, Lingxin.(2018).Evaluation Selenocysteine Protective Effect in Carbon Disulfide Induced Hepatitis with a Mitochondrial Targeting Ratiometric Near-Infrared Fluorescent Probe.ANALYTICAL CHEMISTRY,90(13),8108-8115.
MLAHan, Xiaoyue,et al."Evaluation Selenocysteine Protective Effect in Carbon Disulfide Induced Hepatitis with a Mitochondrial Targeting Ratiometric Near-Infrared Fluorescent Probe".ANALYTICAL CHEMISTRY 90.13(2018):8108-8115.
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