Gao, Zhiwei; Sun, Jingya; Gao, Min; Yu, Fabiao; Chen, Lingxin
; Chen, Qingguo发表期刊SENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
2018-07-15
卷号265页码:565-574
关键词Alkaline phosphataseFluorescent probesNear-infrared fluorescenceIn vivo imaging
研究领域Chemistry; Electrochemistry; Instruments & Instrumentation
DOI10.1016/j.snb.2018.03.078
产权排序[Gao, Zhiwei; Sun, Jingya; Yu, Fabiao; Chen, Lingxin; Chen, Qingguo] Zhejiang Ocean Univ, Coll Marine Sci & Technol, Zhoushan 316004, Peoples R China; [Gao, Zhiwei; Gao, Min; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Gao, Min] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要Alkaline phosphatase (ALP) is a class of enzymes that are widely found in various tissues of the human body. It plays important roles in regulating diverse cellular functions. The aberrant levels of serum ALP are implicated in diseases. The development of sensitive and accurate detection tool for evaluating the level changes of ALP in living organisms will be very helpful in the fields of biochemistry, cytology and clinical medicine. Herein, we develop a near-infrared probe (QcyP) for the specific detection of different ALP levels both in different cell lines and in tumor-bearing mice models. The probe is composed of two moieties: a unique heptamethine cyanine fluorophore and a phosphate monoester. ALP can trigger an off-on fluorescence switch via an enzyme-catalyzed cleavage of the phosphate group. The fluorescence regulation mechanism is based on the rearrangement of the conjugated pi-electron system. The probe exhibits high selectivity and good sensitivity towards ALP. The results demonstrate that the probe QcyP not only can be applied to discriminate the levels of ALP in different cell lines, but also can distinguish the ALP level changes between hepatic tumor mice model and normal mice.
文章类型Article
资助机构National Nature Science Foundation of China [41506126, 21775162, 41776110, 21575159]; program of Youth Innovation Promotion Association, CAS [2015170]; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22]; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology [SATM201705]
收录类别SCI
语种英语
关键词[WOS]RATIOMETRIC FLUORESCENT-PROBE; LIVING CELLS; QUANTUM DOTS; CARBON DOTS; ASSAY; NITROREDUCTASE; NANOPARTICLES; PLATFORMS; PARADIGM; BIOLOGY
研究领域[WOS]Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation
WOS记录号WOS:000430232500068
引用统计被引频次：31[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24437
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Zhejiang Ocean Univ, Coll Marine Sci & Technol, Zhoushan 316004, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Gao, Zhiwei,Sun, Jingya,Gao, Min,et al. A unique off-on near-infrared cyanine-based probe for imaging of endogenous alkaline phosphatase activity in cells and in vivo[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,265:565-574.
APAGao, Zhiwei,Sun, Jingya,Gao, Min,Yu, Fabiao,Chen, Lingxin,&Chen, Qingguo.(2018).A unique off-on near-infrared cyanine-based probe for imaging of endogenous alkaline phosphatase activity in cells and in vivo.SENSORS AND ACTUATORS B-CHEMICAL,265,565-574.
MLAGao, Zhiwei,et al."A unique off-on near-infrared cyanine-based probe for imaging of endogenous alkaline phosphatase activity in cells and in vivo".SENSORS AND ACTUATORS B-CHEMICAL 265(2018):565-574.
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