Liu, Xiaolin; Liu, Wei
; Sun, Yu; Xia, Chunlei; Elmerich, Claudine; Xie, Zhihong发表期刊APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
2018-02
卷号84期号:3页码:UNSP e01827-17
关键词Azorhizobium caulinodansCheZSesbania rostratachemotaxiscolonization
研究领域Biotechnology & Applied Microbiology; Microbiology
DOI10.1128/AEM.01827-17
产权排序[Liu, Xiaolin; Liu, Wei; Sun, Yu; Xie, Zhihong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China; [Liu, Xiaolin; Sun, Yu] Univ Chinese Acad Sci, Beijing, Peoples R China; [Xia, Chunlei] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol Shandong, Yantai, Peoples R China; [Elmerich, Claudine] Inst Pasteur, Paris, France
作者部门海岸带生物学与生物资源保护实验室
英文摘要Chemotaxis can provide bacteria with competitive advantages for survival in complex environments. The CheZ chemotaxis protein is a phosphatase, affecting the flagellar motor in Escherichia coli by dephosphorylating the response regulator phosphorylated CheY protein (CheY similar to P) responsible for clockwise rotation. A cheZ gene has been found in Azorhizobium caulinodans ORS571, in contrast to other rhizobial species studied so far. The CheZ protein in strain ORS571 has a conserved motif similar to that corresponding to the phosphatase active site in E. coli. The construction of a cheZ deletion mutant strain and of cheZ mutant strains carrying a mutation in residues of the putative phosphatase active site showed that strain ORS571 participates in chemotaxis and motility, causing a hyperreversal behavior. In addition, the properties of the cheZ deletion mutant revealed that ORS571 CheZ is involved in other physiological processes, since it displayed increased flocculation, biofilm formation, exopolysaccharide (EPS) production, and host root colonization. In particular, it was observed that the expression of several exp genes, involved in EPS synthesis, was upregulated in the cheZ mutant compared to that in the wild type, suggesting that CheZ negatively controls exp gene expression through an unknown mechanism. It is proposed that CheZ influences the Azorhizobium-plant association by negatively regulating early colonization via the regulation of EPS production. This report established that CheZ in A. caulinodans plays roles in chemotaxis and the symbiotic association with the host plant. IMPORTANCE Chemotaxis allows bacteria to swim toward plant roots and is beneficial to the establishment of various plant-microbe associations. The level of CheY phosphorylation (CheY similar to P) is central to the chemotaxis signal transduction. The mechanism of the signal termination of CheY similar to P remains poorly characterized among Alphaproteobacteria, except for Sinorhizobium meliloti, which does not contain CheZ but which controls CheY similar to P dephosphorylation through a phosphate sink mechanism. Azorhizobium caulinodans ORS571, a microsymbiont of Sesbania rostrata, has an orphan cheZ gene besides two cheY genes similar to those in S. meliloti. In addition to controlling the chemotaxis response, the CheZ-like protein in strain ORS571 is playing a role by decreasing bacterial adhesion to the host plant, in contrast to the general situation where chemotaxis-associated proteins promote adhesion. In this study, we identified a CheZ-like protein among Alphaproteobacteria functioning in chemotaxis and the A. caulinodans-S. rostrata symbiosis.
文章类型Article
资助机构National Natural Science Foundation of China [31370108, 31570063]; One Hundred-Talent Plan of the Chinese Academy of Sciences; High-Tech Industrialization Cooperation Funds of Jilin province; Chinese Academy of Sciences [2017SYHZ0007]; Shandong Key Research and Development Program [2016CYJS05A01-1]; Institut Pasteur, Paris, France
收录类别SCI
语种英语
关键词[WOS]GRAM-NEGATIVE BACTERIA; SINORHIZOBIUM-MELILOTI; SESBANIA-ROSTRATA; ESCHERICHIA-COLI; AZOSPIRILLUM-BRASILENSE; RHIZOBIUM-MELILOTI; BIOFILM FORMATION; CELL-AGGREGATION; SIGNAL-TRANSDUCTION; RESPONSE REGULATOR
研究领域[WOS]Biotechnology & Applied Microbiology; Microbiology
WOS记录号WOS:000423770000008
引用统计被引频次：11[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24401
专题中科院海岸带环境过程与生态修复重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China;
2.Univ Chinese Acad Sci, Beijing, Peoples R China;
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol Shandong, Yantai, Peoples R China;
4.Inst Pasteur, Paris, France
推荐引用方式
GB/T 7714Liu, Xiaolin,Liu, Wei,Sun, Yu,et al. A cheZ-Like Gene in Azorhizobium caulinodans Is a Key Gene in the Control of Chemotaxis and Colonization of the Host Plant[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(3):UNSP e01827-17.
APALiu, Xiaolin,Liu, Wei,Sun, Yu,Xia, Chunlei,Elmerich, Claudine,&Xie, Zhihong.(2018).A cheZ-Like Gene in Azorhizobium caulinodans Is a Key Gene in the Control of Chemotaxis and Colonization of the Host Plant.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,84(3),UNSP e01827-17.
MLALiu, Xiaolin,et al."A cheZ-Like Gene in Azorhizobium caulinodans Is a Key Gene in the Control of Chemotaxis and Colonization of the Host Plant".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 84.3(2018):UNSP e01827-17.
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