Wang, Xiaoyan; Yu, Shuangmei; Liu, Wen; Fu, Longwen
; Wang, Yunqing; Li, Jinhua; Chen, Lingxin发表期刊ACS SENSORS
ISSN2379-3694
2018-02
卷号3期号:2页码:378-385
关键词molecular imprintingprotein imprintingratiometric fluorescencebovine hemoglobinquantum dots
研究领域Chemistry, Multidisciplinary; Nanoscience & Nanotechnology
DOI10.1021/acssensors.7b00804
产权排序[Wang, Xiaoyan] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Wang, Xiaoyan; Liu, Wen; Fu, Longwen; Wang, Yunqing; Li, Jinhua; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Yu, Shuangmei] Yantai Yuhuangding Hosp, Radiotherapy Ward, Yantai 264000, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要We describe a simple and effective strategy to construct a molecular imprinting ratiometric fluorescence sensor (MIR sensor) for the visual detection of bovine hemoglobin (BHb) used as a model protein. The sensor was prepared by simply mixing the solution of green and red CdTe quantum dots (QDs), which were embedded in core-shell structured molecularly imprinted polymers and silica nano-particles, respectively. The resultant hybrid MIR sensor can selectively bind with BHb and thus quench the fluorescence of the green QDs, while the red QDs wrapped with silica are insensitive to BHb with the fluorescence intensity unchanged. As a result, a continuous obvious fluorescence color change from green to red can be observed by naked eyes, with the detection limit of 9.6 nM. Moreover, the MIR sensor was successfully applied to determine BHb in bovine urine samples with satisfactory recoveries at three spiking levels ranging from 95.7 to 101.5%, indicating great potential application for detecting BHb in real samples. This strategy of using different fluorescence emission materials incorporated to construct a ratiometric fluorescence sensor is reasonable and convenient, which can be extended to the preparation of other ratiometric fluorescence systems for targeted analytes.
文章类型Article
资助机构National Natural Science Foundation of China [21477160, 21575159, 81573393]; Science and Technology Development Plan of Shandong Province of China [2014GGF01074]; Youth Innovation Promotion Association CAS [2017256]; Defense Science and Technology Innovation Fund of the Chinese Academy of Sciences [CXJJ-17-Q148]; Instrument Developing Project of the Chinese Academy of Sciences
收录类别SCI
语种英语
关键词[WOS]CDTE QUANTUM DOTS; DOPED ZNS QDS; TARGET PROTEIN; SENSITIVE DETECTION; ALPHA-FETOPROTEIN; SERUM-ALBUMIN; TURN-ON; POLYMER; RECOGNITION; SURFACE
研究领域[WOS]Chemistry; Science & Technology - Other Topics
WOS记录号WOS:000426618400017
引用统计被引频次：100[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24397
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
3.Yantai Yuhuangding Hosp, Radiotherapy Ward, Yantai 264000, Peoples R China
推荐引用方式
GB/T 7714Wang, Xiaoyan,Yu, Shuangmei,Liu, Wen,et al. Molecular Imprinting Based Hybrid Ratiometric Fluorescence Sensor for the Visual Determination of Bovine Hemoglobin[J]. ACS SENSORS,2018,3(2):378-385.
APAWang, Xiaoyan.,Yu, Shuangmei.,Liu, Wen.,Fu, Longwen.,Wang, Yunqing.,...&Chen, Lingxin.(2018).Molecular Imprinting Based Hybrid Ratiometric Fluorescence Sensor for the Visual Determination of Bovine Hemoglobin.ACS SENSORS,3(2),378-385.
MLAWang, Xiaoyan,et al."Molecular Imprinting Based Hybrid Ratiometric Fluorescence Sensor for the Visual Determination of Bovine Hemoglobin".ACS SENSORS 3.2(2018):378-385.
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