Ding, Jiawang
; Yu, Nana; Wang, Xuedong; Qin, Wei发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
2018-02-06
卷号90期号:3页码:1734-1739
关键词PULSED GALVANOSTATIC CONTROLPOLYMERIC MEMBRANEELECTROCHEMICAL DETECTIONLIQUID-MEMBRANELABEL-FREESENSORSSUBSTRATEPRINCIPLEPROTAMINEHEPARIN
研究领域Chemistry, Analytical
DOI10.1021/acs.analchem.7b03522
产权排序[Ding, Jiawang; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China; [Ding, Jiawang; Qin, Wei] YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China; [Yu, Nana; Wang, Xuedong] Wenzhou Med Univ, Key Lab Watershed Sci & Hlth Zhejiang Prov, Dept Environm Sci, Wenzhou 325035, Peoples R China
作者部门海岸带环境过程实验室
英文摘要A polymeric membrane ion-selective electrode (ISE) is typically designed for the determination of one specific ion using a conventional method. In this work, we demonstrate a simple, versatile, and sensitive platform for simultaneous detection of two molecules with a single ISE. Under a series of periodic galvanostatic polarization, a solid-contact ISE without ion exchanger properties under zero-current conditions has been successfully used for simultaneous detection of two opposite charged ions with high sensitivity, good selectivity, and fast reversibility. By integration of biorecognition elements with the potentiometric measurement, highly sensitive and selective detection of a broad range of different molecular targets can be predicted. As a proof of concept, a potentiometric genosensor based on magnetic beads-enzyme sandwich assay has been designed for sensitive and selective detection of pathogenic bacteria Escherichia coli O157:H7 and Staphylococcus aureus. Under optimal conditions, two bacteria nucleic acid sequences can be detected simultaneously with high sensitivity and good selectivity by using a single solid-contact potentiometric ISE. The detection limits of Escherichia coli O157:H7 DNA and Staphylococcus aureus DNA are 120 and 54 fM (3 sigma), respectively. Because of its simplicity, this potentiometric technique based on ISE can be an attractive tool or detector to perform two analyte measurements.
文章类型Article
资助机构National Natural Science Foundation of China [21575158, 21677172]; Instrument Developing Project of the Chinese Academy of Sciences [Y728021021]; National Key Research and Development Program of China [2016YFC1400700]; Taishan Scholar Program of Shandong Province [TS20081159]
收录类别SCI
语种英语
关键词[WOS]PULSED GALVANOSTATIC CONTROL; POLYMERIC MEMBRANE; ELECTROCHEMICAL DETECTION; LIQUID-MEMBRANE; LABEL-FREE; SENSORS; SUBSTRATE; PRINCIPLE; PROTAMINE; HEPARIN
研究领域[WOS]Chemistry
WOS记录号WOS:000424730600043
引用统计被引频次：17[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24396
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China;
2.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China;
3.Wenzhou Med Univ, Key Lab Watershed Sci & Hlth Zhejiang Prov, Dept Environm Sci, Wenzhou 325035, Peoples R China
推荐引用方式
GB/T 7714Ding, Jiawang,Yu, Nana,Wang, Xuedong,et al. Sequential and Selective Detection of Two Molecules with a Single Solid-Contact Chronopotentiometric Ion-Selective Electrode[J]. ANALYTICAL CHEMISTRY,2018,90(3):1734-1739.
APADing, Jiawang,Yu, Nana,Wang, Xuedong,&Qin, Wei.(2018).Sequential and Selective Detection of Two Molecules with a Single Solid-Contact Chronopotentiometric Ion-Selective Electrode.ANALYTICAL CHEMISTRY,90(3),1734-1739.
MLADing, Jiawang,et al."Sequential and Selective Detection of Two Molecules with a Single Solid-Contact Chronopotentiometric Ion-Selective Electrode".ANALYTICAL CHEMISTRY 90.3(2018):1734-1739.
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