Han, Xiaoyue; Song, Xinyu; Li, Bowei
; Yu, Fabiao; Chen, Lingxin发表期刊BIOMATERIALS SCIENCE
ISSN2047-4830
2018-03-01
卷号6期号:3页码:672-682
关键词TURN-ON PROBEHYDROGEN-SULFIDEIN-VIVOCHEMICAL BIOLOGYOXIDATIVE STRESSRATIONAL DESIGNSYNTHASE CBSFAR-REDPOLYSULFIDESCHEMISTRY
研究领域Materials Science, Biomaterials
DOI10.1039/c7bm00951h
产权排序[Han, Xiaoyue; Song, Xinyu; Li, Bowei; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Han, Xiaoyue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yu, Fabiao; Chen, Lingxin] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Minist Educ, Key Lab Sensor Anal Tumor Marker, Qingdao 266042, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要Sulfane sulfur refers to ionized sulfur that is reversibly attached to other sulfur atoms in the form of 6-valence electrons (S-0). Sulfane sulfur possesses stronger nucleophilicity and reducibility than hydrogen sulfide in a series of physiological reactions, which probably makes sulfane sulfur the actual signal molecule in cells. Herein, we designed and synthesized a near-infrared (NIR) fluorescent probe BD-diSH for sensitive detection and imaging of sulfane sulfur in living cells and in vivo. The probe BD-diSH is composed of two moieties: the fluorophore azo-BODIPY and the sulfane sulfur recognition unit, viz., 2-mercapto benzoate. BD-diSH displayed high sensitivity and selectivity towards sulfane sulfur. The mercapto group (-SH) of 2-mercapto benzoic acid can nucleophilically capture the sulfur atom of thiosulfoxide tautomers in sulfane sulfur to form -SSH. The group -SSH will immediately induce intramolecular cyclization reaction and release the azo-BODIPY fluorophore to emit NIR fluorescence. The probe BD-diSH was successfully applied to detect and image sulfane sulfur in the cytoplasm of the living cells. The results illustrated that the endogenous and exogenous sulfane sulfur level changed depending on different cell lines. BD-diSH was also capable of imaging the level changes of sulfane sulfur in mice. The above applications make our new probe a potential chemical tool for the study of physiological and pathological functions of sulfur sulfide in living cells and in vivo.
文章类型Article
资助机构State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22]; National Nature Science Foundation of China [21775162, 21405172, 21575159]; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology [SATM201705]; program of Youth Innovation Promotion Association, CAS [2015170]
收录类别SCI
语种英语
关键词[WOS]TURN-ON PROBE; HYDROGEN-SULFIDE; IN-VIVO; CHEMICAL BIOLOGY; OXIDATIVE STRESS; RATIONAL DESIGN; SYNTHASE CBS; FAR-RED; POLYSULFIDES; CHEMISTRY
研究领域[WOS]Materials Science
WOS记录号WOS:000426392200021
引用统计被引频次：16[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24385
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
3.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Minist Educ, Key Lab Sensor Anal Tumor Marker, Qingdao 266042, Peoples R China
推荐引用方式
GB/T 7714Han, Xiaoyue,Song, Xinyu,Li, Bowei,et al. A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and &ITin vivo&IT[J]. BIOMATERIALS SCIENCE,2018,6(3):672-682.
APAHan, Xiaoyue,Song, Xinyu,Li, Bowei,Yu, Fabiao,&Chen, Lingxin.(2018).A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and &ITin vivo&IT.BIOMATERIALS SCIENCE,6(3),672-682.
MLAHan, Xiaoyue,et al."A near-infrared fluorescent probe for sensitive detection and imaging of sulfane sulfur in living cells and &ITin vivo&IT".BIOMATERIALS SCIENCE 6.3(2018):672-682.
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