Gao, Min; Wang, Rui; Yu, Fabiao; Chen, Lingxin
发表期刊BIOMATERIALS
ISSN0142-9612
2018-04
卷号160页码:1-14
关键词Fluorescent probesSulfane sulfurMitochondria-targetingIn vivo bioimagingHypoxia stress
研究领域Engineering, Biomedical; Materials Science, Biomaterials
DOI10.1016/j.biomaterials.2018.01.011
产权排序[Gao, Min; Wang, Rui; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Gao, Min] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要As a crucial member in antioxidant regulatory systems, sulfane sulfur plays essential roles in cytoprotective mechanisms by directly eliminating ROS and altering ROS-mediated redox signaling. Despite the rising interests in sulfane sulfur, there only a few bio-compatible methods are available for its direct detection. Moreover, most of the existing methods cannot meet the requirements of real-time detection due to the reactive and labile chemical properties of sulfane sulfur. Therefore, we strive to clarify the mutual relationship between mitochondria sulfane sulfur and ROS under hypoxia stress. Herein, we report a near-infrared fluorescent probe Mito-SeH for the selective imaging of mitochondrial sulfane sulfur in cells and in vivo under hypoxia stress. Mito-SeH includes three moieties: a selenol group (-SeH) as the stronger sulfur-acceptor; a near-infrared azo-BODIPY fluorophore as the fluorescent modulator; a lipophilic alkyltriphenylphosphonium cation as the mitochondrial delivery. Mito-SeH exhibits excellent selectivity and sensitivity towards the detection of mitochondria sulfane sulfur. The hypoxia response behavior of Mito-SeH is evaluated in monolayer cell and three-dimensional multicellular spheroid to clarify the relationship between sulfane sulfur and hypoxia. We confirm that sulfane sulfur protection mechanism against hypoxia is to inhibition of caspase-dependent apoptosis through directly scavenging ROS pathway. The probe is also applied to measurement of sulfane sulfur in ex vivo-dissected organs of hypoxic mouse model, as well as the probe is successfully used for real-time monitoring the changes of sulfane sulfur and ROS in acute ischemia mice model. We suggest that sulfane sulfur may be a novel therapeutic agent for hypoxia-induced injury. (C) 2018 Elsevier Ltd. All rights reserved.
文章类型Article
资助机构National Nature Science Foundation of China [21405172, 21775162, 21575159, 21522706]; program of Youth Innovation Promotion Association, CAS [2015170]; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS [KF2016-22]
收录类别SCI
语种英语
关键词[WOS]HYDROGEN-SULFIDE; IN-VIVO; 3-MERCAPTOPYRUVATE SULFURTRANSFERASE; BIOLOGICAL-SYSTEMS; OXIDATIVE STRESS; CHEMICAL BIOLOGY; REGULATORY ROLE; LIVING CELLS; HYPOXIA; POLYSULFIDES
研究领域[WOS]Engineering; Materials Science
WOS记录号WOS:000425573400001
引用统计被引频次：55[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24381
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Gao, Min,Wang, Rui,Yu, Fabiao,et al. Evaluation of sulfane sulfur bioeffects via a mitochondria-targeting selenium-containing near-infrared fluorescent probe[J]. BIOMATERIALS,2018,160:1-14.
APAGao, Min,Wang, Rui,Yu, Fabiao,&Chen, Lingxin.(2018).Evaluation of sulfane sulfur bioeffects via a mitochondria-targeting selenium-containing near-infrared fluorescent probe.BIOMATERIALS,160,1-14.
MLAGao, Min,et al."Evaluation of sulfane sulfur bioeffects via a mitochondria-targeting selenium-containing near-infrared fluorescent probe".BIOMATERIALS 160(2018):1-14.
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