Sun, Xiange; Li, Bowei
; Tian, Chunyuan; Yu, Fabiao; Zhou, Na; Zhan, Yinghua; Chen, Lingxin发表期刊ANALYTICA CHIMICA ACTA
ISSN0003-2670
2018-05-12
卷号1007页码:33-39
关键词Microfluidic paper-based deviceElectrochemiluminescence immunoassayValveCancer biomarkerPoint-of-care
研究领域Chemistry, Analytical
DOI10.1016/j.aca.2017.12.005
产权排序[Sun, Xiange; Li, Bowei; Yu, Fabiao; Zhou, Na; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Tian, Chunyuan] Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China; [Sun, Xiange] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zhan, Yinghua] Yantai Xinhua Hlth Outpatient Dept, Yantai 264003, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要This paper describes a novel rotational paper-based analytical device (RPAD) to implement multi-step electrochemiluminescence (ECL) immunoassays. The integrated paper-based rotational valves can be easily controlled by rotating paper discs manually and this advantage makes it user-friendly to untrained users to carry out the multi-step assays. In addition, the rotational valves are reusable and the response time can be shortened to several seconds, which promotes the rotational paper-based device to have great advantages in multi-step operations. Under the control of rotational valves, multi-step ECL immunoassays were conducted on the rotational device for the multiplexed detection of carcinoembryonic antigen (CEA) and prostate specific antigen (PSA). The rotational device exhibited excellent analytical performance for CEA and PSA, and they could be detected in the linear ranges of 0.1-100 ngmL(-1) and 0.1-50 ng mL(-1) with detection limits down to 0.07 ng mL(-1) and 0.03 ng mL(-1), respectively, which were within the ranges of clinical concentrations. We hope this technique will open a new avenue for the fabrication of paper-based valves and provide potential application in clinical diagnostics. (C) 2017 Elsevier B.V. All rights reserved.
文章类型Article
资助机构National Natural Science Foundation of China [41776110, 21205131, 21775162]; Science and Technology Development Plan of Yantai [2015ZH087]; National Key Research and Development Program of China [2016YFC1400702]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
收录类别SCI
语种英语
关键词[WOS]ANALYTICAL DEVICES; MICROFLUIDIC CHIP; FLUORESCENCE DETECTION; IMMUNOASSAY; FLOW; INTEGRATION; DIAGNOSTICS; STRATEGY; PROGRESS; ARRAYS
研究领域[WOS]Chemistry
WOS记录号WOS:000423843500005
引用统计被引频次：67[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24370
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China;
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
4.Yantai Xinhua Hlth Outpatient Dept, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Sun, Xiange,Li, Bowei,Tian, Chunyuan,et al. Rotational paper-based electrochemiluminescence immunodevices for sensitive and multiplexed detection of cancer biomarkers[J]. ANALYTICA CHIMICA ACTA,2018,1007:33-39.
APASun, Xiange.,Li, Bowei.,Tian, Chunyuan.,Yu, Fabiao.,Zhou, Na.,...&Chen, Lingxin.(2018).Rotational paper-based electrochemiluminescence immunodevices for sensitive and multiplexed detection of cancer biomarkers.ANALYTICA CHIMICA ACTA,1007,33-39.
MLASun, Xiange,et al."Rotational paper-based electrochemiluminescence immunodevices for sensitive and multiplexed detection of cancer biomarkers".ANALYTICA CHIMICA ACTA 1007(2018):33-39.
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