Dou, K; Fu, Q; Chen, G; Yu, FB; Liu, YX; Cao, ZP; Li, GL; Zhao, XN; Xia, L; Chen, LX
; Wang, H; You, JM发表期刊BIOMATERIALS
ISSN0142-9612
2017-07
卷号133页码:82-93
关键词Dual-responseDual-ratiometricQuantificationSO2/ClO-Imaging analysis
研究领域Engineering, Biomedical; Materials Science, Biomaterials
DOI10.1016/j.biomaterials.2017.04.024
产权排序[Dou, Kun; Fu, Qiang; Chen, Guang; Liu, Yuxia; Cao, Ziping; Li, Guoliang; Zhao, Xianen; Xia, Lian; Wang, Hua; You, Jinmao] Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China; [Chen, Guang] Shandong Normal Univ, Engn Res Ctr Pesticide & Med Intermediate Clean P, Chem Engn & Mat Sci, Coll Chem,Minist Educ, Jinan 250014, Peoples R China; [Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remedia, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要Intracellular reactive sulfur species and reactive oxygen species play vital roles in immunologic mechanism. As an emerging signal transmitter, SO2 can be generated as the anti-oxidant, while SO2 is also a potential oxidative stress-inducer in organism. Aiming to elucidate in-depth the dichotomous role of SO2 under oxidative stress, we designed a dual-response fluorescent probe that enabled the respective or successive detection of SO2 and ClO-. The probe itself emits the red fluorescence (625 nm) which can largely switch to blue (410 nm) and green fluorescence (500 nm) respectively in response to SO2 and ClO-, allowing the highly selective and accurate ratiometric quantification for both SO2 and ClO- in cells. Moreover the ultrafast (SO2: <60 s; ClO-: within sec) and highly sensitive (detection limits: SO2: 3.5 nM; ClO-: 12.5 nM) detection were achieved. With the robust applicability, the developed probe was successfully used to quantify SO2 and endogenous ClO- in respectively the HeLa cells and the RAW 264.7 cells, as well as to visualize the dynamic of SO2/ClO- in zebrafish. The fluorescent imaging studies and flow cytometry analysis confirmed the burst-and-depletion and meanwhile the oxidative-and-antioxidative effects of intracellular SO2 under the NaClO induced oxidative stress. (C) 2017 Elsevier Ltd. All rights reserved.
文章类型Article
资助机构National Natural Science Foundation of China [21475074, 21403123, 21375075, 31301595, 21402106, 21475075, 21275089, 21405093, 21405094, 81303179, 21305076]; Shandong Province Key Laboratory of Detection Technology for Tumor Markers [KLDTTM2015-6]; Opening Foundation of Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers [KLDTTM2015-9]; Taishan Scholar Foundation of Shandong Province, PR China
收录类别SCI
语种英语
关键词[WOS]Dual-response; Dual-ratiometric; Quantification; SO2/ClO-; Imaging analysis
研究领域[WOS]Engineering; Materials Science
WOS记录号WOS:000401716700009
引用统计被引频次：102[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24362
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Qufu Normal Univ, Key Lab Pharmaceut Intermediates & Anal Nat Med, Key Lab Life Organ Anal, Qufu 273165, Shandong, Peoples R China;
2.Shandong Normal Univ, Engn Res Ctr Pesticide & Med Intermediate Clean P, Chem Engn & Mat Sci, Coll Chem,Minist Educ, Jinan 250014, Peoples R China;
3.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remedia, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Dou, K,Fu, Q,Chen, G,et al. A novel dual-ratiometric-response fluorescent probe for SO2/ClO- detection in cells and in vivo and its application in exploring the dichotomous role of SO2 under the ClO- induced oxidative stress[J]. BIOMATERIALS,2017,133:82-93.
APADou, K.,Fu, Q.,Chen, G.,Yu, FB.,Liu, YX.,...&You, JM.(2017).A novel dual-ratiometric-response fluorescent probe for SO2/ClO- detection in cells and in vivo and its application in exploring the dichotomous role of SO2 under the ClO- induced oxidative stress.BIOMATERIALS,133,82-93.
MLADou, K,et al."A novel dual-ratiometric-response fluorescent probe for SO2/ClO- detection in cells and in vivo and its application in exploring the dichotomous role of SO2 under the ClO- induced oxidative stress".BIOMATERIALS 133(2017):82-93.
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