Dou, K; Chen, G; Yu, FB; Sun, ZW; Li, GL; Zhao, XE; Chen, LX
; You, JM发表期刊JOURNAL OF MATERIALS CHEMISTRY B
ISSN2050-750X
2017-11-14
卷号5期号:42页码:8389-8398
关键词SULFUR-DIOXIDE DERIVATIVESLIVING CELLSHYPOCHLOROUS ACIDOXIDATIVE STRESSHYDROGEN-SULFIDEBIOIMAGING APPLICATIONSPARKINSONS-DISEASEREACTIVE OXYGENLIVE-CELLTISSUES
研究领域Materials Science, Biomaterials
DOI10.1039/c7tb01900a
产权排序[Dou, Kun; Chen, Guang; Yu, Fabiao; Sun, Zhiwei; Li, Guoliang; Zhao, Xianen; Chen, Lingxin; You, Jinmao] Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Peoples R China; [Dou, Kun; Chen, Guang; Yu, Fabiao; Chen, Lingxin; You, Jinmao] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要Known as a new member of the reactive sulphur species (RSS), endogenous sulfur dioxide (SO2) plays potentially reductive roles such as antioxidation, anti-aging, anti-inflammation, and cytoprotection; however, SO2 is also a metabolite of antioxidation. Hypochlorous acid (HClO) has powerful bio-effects on the innate immune system, and its uncontrolled production leads to adverse damage in cells. To illuminate the potential crosstalk between SO2 and HClO in redox homeostasis, we designed a two-photon ratiometric fluorescent probe for dual-response to mitochondrial SO2/HClO crosstalk in cells and in vivo. Our probe can effectively achieve ratio-type fluorescence dual-response to SO2 and HClO with desirable properties such as large two-photon absorption cross sections, rapid response times (SO2 , 50 s, HClO < 20 s), high sensitivities (limit of detection 8.0 nM for SO2 and 15.2 nM for HClO), and favorable selectivity. The probe has been successfully applied to quantitatively detect endogenous SO2 and HClO in HeLa and Raw 264.7 cells. We have verified that there exists a crosstalk between SO2 and HClO during the process of oxidative stress in mitochondria. We have also detected that SO2 can be endogenously produced through oxidation of intracellular sulfur-containing amino acids by HClO in zebrafish in real-time.
文章类型Article
资助机构National Natural Science Foundation of China [21475074, 21403123, 21375075, 31301595, 21402106, 21475075, 21275089, 21405093, 21405094, 81303179, 21305076]; Funds of Shandong Province Key Laboratory of Detection Technology for Tumour Markers [KLDTTM2015-6, KLDTTM2015-9]
收录类别SCI
语种英语
关键词[WOS]SULFUR-DIOXIDE DERIVATIVES; LIVING CELLS; HYPOCHLOROUS ACID; OXIDATIVE STRESS; HYDROGEN-SULFIDE; BIOIMAGING APPLICATIONS; PARKINSONS-DISEASE; REACTIVE OXYGEN; LIVE-CELL; TISSUES
研究领域[WOS]Materials Science
WOS记录号WOS:000414156300011
引用统计被引频次：54[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24346
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Dou, K,Chen, G,Yu, FB,et al. A two-photon ratiometric fluorescent probe for the synergistic detection of the mitochondrial SO2/HClO crosstalk in cells and in vivo[J]. JOURNAL OF MATERIALS CHEMISTRY B,2017,5(42):8389-8398.
APADou, K.,Chen, G.,Yu, FB.,Sun, ZW.,Li, GL.,...&You, JM.(2017).A two-photon ratiometric fluorescent probe for the synergistic detection of the mitochondrial SO2/HClO crosstalk in cells and in vivo.JOURNAL OF MATERIALS CHEMISTRY B,5(42),8389-8398.
MLADou, K,et al."A two-photon ratiometric fluorescent probe for the synergistic detection of the mitochondrial SO2/HClO crosstalk in cells and in vivo".JOURNAL OF MATERIALS CHEMISTRY B 5.42(2017):8389-8398.
PDF全文下载地址:
点我下载PDF
