086000|生物与医药专业硕士研究生指导教师
姓名 高娟 性别 女
民族 汉 出生年月 1985.11
学历|学位 研究生|博士 专业技术职务 讲师
行政职务 讲师
联系电话 ** 移动电话 **
邮箱 bio_gaoj@ujn.edu.cn
通讯地址|邮编 济南市南辛庄西路336号生科院|250000
招生方向(领域) 生物与医药
研究领域、学习工作经历、学术兼职等情况 2003.09-2007.06： 山东科技大学 生物工程专业 本科2007.09-2012.06： 东北师范大学 生物化学专业 硕博连读研究生2010.12-2011.11： 加拿大国家研究理事会生物科学研究所 联合培养博士生

代表性科研成果及奖励：
（包括项目、鉴定、论文、专著、专利等) 期刊论文：
Ye Yuan, Li Ji, Yanbo Hu, Chenxing Hu, Honglei Chen, Juan Gao^*(通讯作者), Yifa Zhou^*. High yield preparation of ganglioside GM1 using recombinant sialidase from Cellulosimicrobium cellulans. Process Biochemistry, 2017, 58， 92-97.
Ye Yuan, Yanbo Hu, Han Zhang, Jiayi Leng, Fan Li, Xuesong Zhao, Juan Gao^* (通讯作者), Yifa Zhou^*. Characterization of a recombinant multifunctional glycoside hydrolase family 3 β-xylosidase/α-L-arabinofuranosidase/β-glucosidase from Cellulosimicrobium cellulans sp. 21. Journal of Molecular Catalysis B: Enzymatic, 2016, 131, 65-72.
Nan Wang¹, Annoor Awadasseid¹, Ye Yuan, Jing Cui, Te Guo, Dongxia Gou, Yeming Bai, Yifa Zhou, Juan Gao* (通讯作者). Biotransformation of ginsenoside Rb1 for ginsenoside Rd preparation by Lysinibacillus massiliensis NO.24. Indian Journal of Biotechnology 2016, 15, 400-406.
Yumei Li^*, Qiang Li, Yamei Li, Juan Gao, Xiangyu Fan. Draft Genome Sequence of Paenibacillus polymyxa KF-1, an Excellent Producer of Microbicides. Genome Announcement, 2016, 4(4): e00727-16.
Yumei Li^*, Yamei Li, Qiang Li, Xiangyu Fan, Juan Gao, Yan Luo. Rapid Biosynthesis of Gold Nanoparticles by the Extracellular Secretion of Bacillus niabensis 45: Characterization and Antibiofilm Activity. Journal of Chemistry, 2016, http://dx.doi.org/10.1155/2016/**.
Ye Yuan, Yanbo Hu, Chenxing Hu, Jiayi Leng, Honglei Chen, Xuesong Zhao, Juan Gao* (通讯作者), Yifa Zhou*. Overexpression and characterization of a glycoside hydrolase family 1 enzyme from Cellulosimicrobium cellulans sp.21 and its application for minor ginsenosides production. Journal of Molecular Catalysis B: Enzymatic, 2015, 120, 60-67.
Juan Gao¹, Yanbo Hu¹, Yue Meng, Fanlin Meng, Xiaoqing Guo, Nan Wang, Min Wei, Yifa Zhou*. Simple and efficient preparation of ginsenoside (S)-Rg2 from ginsenoside Re by biotransformation of Cellulosimicrobium sp. 21. Biocatalysis and Biotransformation, 2015, 33(1), 51-60.
Juan Gao, Jiao Wang, Jing Cui, Nan Wang, Yeming Bai, Ye Yuan, Yifa Zhou*. Purification and characterization of two novel β-glucosidases from Penicillium oxalicum and their application in bioactive ginsenosides production. Biocatalysis and Biotransformation, 2014, 32(4), 199-2074.
Juan Gao, Warren Wakarchuk*. Characterization of Five β-Glycoside Hydrolases from Cellulomonas fimi ATCC484. Journal of Bacteriology, 2014, 196(23), 4103-4110.
Juan Gao, Yanbo Hu, Li Ji, Nan Wang, Jiao Wang, Guihua Tai, Yifa Zhou*. A novel ginsenoside-hydrolyzing enzyme from Penicillium oxalicum and its application in ginsenoside Rd production. Biocatalysis and Biotransformation, 2013, 31(6), 305–312.
Juan Gao, Wenjing Xu, Fei Liang, Miao Hao, Xiaoyu Liu, Ling Fu, Rutian Jin, Nan Wang, Guihua Tai, Yifa Zhou*. Efficient biotransformation for preparation of pharmaceutically active ginsenoside Compound K by Penicillium oxalicum sp.68. Annals of Microbiology, 2013, 63, 139-149.
Xuesong Zhao, Juan Gao, Chengcheng Song, Qiang Fang, Nan Wang, Tianjiao Zhao, Dongbo Liu, Yifa Zhou*. Fungal sensitivity to and enzymatic degradation of the ginsenosides. Phytochemistry, 2012, 78, 65-71.
Rutian Jin, Juan Gao, Xiaoyu Liu, Wei Yang, Xiaoxia Peng, Tao Zhang, Yifa Zhou*. Ginsenoside Rg3: Spectral Analysis and Sensitive Detection with Silver Nanoparticles Decorated Graphene. Science of Advanced Materials, 2012, 4, 1160-1165.
Juan Gao¹, Xuesong Zhao¹, Haibo Liu, Yuying Fan, Hairong Cheng, Fei Liang, Xingxing Chen, Nan Wang, Yifa Zhou*, Guihua Tai*. A highly selective ginsenoside Rb1-hydrolyzing β-D-glucosidase from Cladosporium fulvum. Process Biochemistry, 2010, 45, 897-903.
Xuesong Zhao, Ling Gao, Juan Wang, Hongtao Bi, Juan Gao, Xiuli Du, Yifa Zhou*, Guihua Tai*. A novel ginsenoside Rb1-hydrolyzing β-D-glucosidase from Cladosporium fulvum. Process Biochemistry, 2009, 44, 612–618.
Xuesong Zhao, Juan Wang, Jie Li, Ling Fu, Juan Gao, Xiuli Du, Hongtao Bi, Yifa Zhou* and Guihua Tai*. Highly selective biotransformation of ginsenoside Rb1 to Rd by the phytopathogenic fungus Cladosporium fulvum (syn. Fulvia fulva). Journal of Industrial Microbiology and Biotechnology, 2009, 36, 721–726.
高娟*，周安东，原野，赵天蛟. 黑曲霉降解人参皂苷Rb1制备稀有皂苷Compound K.生物技术进展，6(2), 98-104, 2016.
赵雪淞*, 杜秀丽, 高娟. 人参锈腐病菌毁灭柱孢菌酶促降解人参皂苷研究.中国生物防治学报, 4, 017, 2012.
会议论文：
Li Ji, Juan Gao, Yifa Zhou. Purification and characterization of three ginsenoside hydrolyzing β-glycosidases from Penicillium oxalicum. 2012 International Conference on Ginseng.
Nan Wang, Juan Gao, Yifa Zhou. Biotransformation of ginsenosides by Cellulosimicrobium sp. 21. 2012 International Conference on Ginseng.
Tianjiao Zhao, Juan Gao, Yifa Zhou. Transformation of ginsenoside Rb1 by commercial available glycosidases. 2012 International Conference on Ginseng.
高娟，梁飞，付玲，陈星星，周义发，台桂花。草酸青霉对人参二醇型皂甙的转化。东北三省及内蒙古生物化学与分子学会2010年会.
专利：
1. 高娟，周义发，胡彦波，勾东霞，郭特，台桂花。一种具有唾液酸水解酶活性的蛋白质及其应用。授权号：ZL0.5.
2. 高娟，李强，李玉梅。一种酶催化反应罐。授权号：ZL9.X.
3. 高娟，李强，李玉梅。一种用于发酵罐的转种补料装置。授权号：ZL8.5.
4. 周义发，高娟，原野，及莉，冷佳益，陈红磊，台桂花。一种制备单唾液酸四己糖神经节苷脂的方法。授权号：ZL6.0.
5. 周义发，高娟，原野，及莉，台桂花。一种具有唾液酸水解酶活性的蛋白质的制备方法。授权号：ZL0.1.
6. 周义发，高娟，原野，胡彦波，孙成新，陈红磊，胡晨星。一种重组糖苷水解酶用于生物转化制备人参二醇型皂苷的工艺。申请号：3.5.
7. 周义发，高娟，王楠，王姣，孙成新，台桂花。一种生物转化制备稀有人参皂苷Rg2的方法。申请号：0.4.
获奖情况：
1. 周义发，台桂花，程海荣，高娟，范玉莹，崔思思.2014年吉林省自然科学一等奖.

教学及研究生培养情况 本科生《生物化学》理论及实践教学、研究生《酶工程》的授课计划。

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