【个人简介】

姓 名 姜 玮



职 称 教授 博师生导师



行政职务 学院党委书记



学术团体兼职 山东大学化学与化工学院学术委员会委员

山东大学基础科学学部学术委员会委员

中国化学会有机分析专业委员会委员

山东省化学化工学会常务理事



工作单位 分析化学研究所



电 话 **(Lab), **(O)



E-mail wjiang@sdu.edu.cn


【学习及工作经历】??
1984.07 毕业于山东大学化学系，获理学学士学位并留校任教，分别于1993年和2003年在山东大学获理学硕士、博士学位




1984.07 － 1991.12 山东大学化学系，助教




1991.12 － 1997.09 山东大学化学与化工学院，讲师




1997.09 － 2002.09 山东大学化学与化工学院，副教授




2002.09 － 现在 山东大学化学与化工学院，教授




2006.06 － 现在 山东大学化学与化工学院，教授，博士生导师




1999.11 － 2003.12 山东大学化学与化工学院，副院长




2004.01 － 2007.12 山东大学研究生院，副院长




2004.06 － 2007.12 兼任山东大学威海分校研究生处，处长




2007.12 － 2013.01 山东大学化学与化工学院，党委书记兼副院长




2013.01 － 现在 山东大学化学与化工学院，党委书记



【主讲课程】??



分析化学，无机及分析化学，分析化学试验，无机及分析化学试验，分析化学进展等






【研究领域和兴趣】??



DNA纳米材料的设计、制备、表征与生物分析应用


基于荧光显微成像技术的单分子检测、单细胞分析


基于荧光信号放大策略的生物分子超灵敏检测技术


新型纳米荧光生物传感器的设计、构建与应用






【主要论著】60.Modular nuclease-responsive DNA three-way junctions-based dynamic assembly of DNA device and its sensing application
Analytical Chemistry, 2016, Accepted


59.A unique dual recognition hairpin probe mediated fluorescent amplification method for sensitive detection of uracil-DNA glycosylase and endonuclease IV activities
Analyst，2016 Accepted


58.Au Nanoparticles Fluorescence Switch-mediated Target Recycling Amplification Strategy for Sensitive Nucleic Acid Detection
RSC Advances, 2016 Accepted


57.Cascade Signal Amplification Based on Copper Nanoparticle-Reported Rolling Circle Amplification for Ultrasensitive Electrochemical Detection of the Prostate Cancer Biomarker
ACS Applied Materials & Interfaces，2016，Accepted


56.Multiplexed detection of cytokines based on dual bar-code strategy and single-molecule counting
Analytical Chemistry, 2016, Accepted


55.Target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy for the sensitive assay of DNA methyltransferase activity
Chemical Communications, 2015, Accepted


54.Label-free detection of nicotinamide adenine dinucleotide based on ligation-triggered exonuclease III-assisted signal amplification
RSC Advances, 2015 Accepted


53.A dual amplification fluorescent strategy for sensitive detection of DNA methyltransferase activity based on strand displacement amplification and DNAzyme amplification
Biosensors and Bioelectronics, 2015 Accepted


52.Label-free and enzyme-free detection of transcription factors with graphene oxide fluorescence switch-based multifunctional G-quadruplex-hairpin probe
Biosensors and Bioelectronics，2015 Accepted


51.Label-free Molecular Beacons-Based Quadratic Isothermal exponential Amplification: A simple and Sensitive One-Pot Method to Detection DNA Methyltransferase Activity
Chemical Communications, 2015, 51, 13538 - 13541


50.Hairpin assembly circuit-based fluorescence cooperative amplification strategy for enzyme-free and label-free detection of small molecule
Talanta, 2015, 143: 101–106


49.FokI cleavage–inhibition strategy for the specific and accurate detection of transcription factors
Talanta 2015,144, 44–50


48.Label-free and dual-amplified detection of protein via small molecule-ligand linked DNA and a cooperative DNA machine
Biosensors and Bioelectronics, 2015, 72, 107–113


47.Highly selective and sensitive detection of miRNA based on toehold-mediated strand displacement reaction and DNA tetrahedron substrate
Biosensors and Bioelectronics, 2015, 71, 401–406


46.Label-free fluorescence dual-amplified detection of adenosine based on exonucleaseIII-assisted DNA cycling and hybridization chain reaction
Biosensors and Bioelectronics, 2015, 70, 15–20


45.Label-free Nucleic Acid Detection Based on DNA Templated Silver Nanoclusters Fluorescent Probe
Talanta, 2015, 138, 163–168


44.A DNA machine-based fluorescence amplification strategy for sensitive detection of uracil-DNA glycosylase activity
Biosensors and Bioelectronics, 2015, 68, 654-659


43.A Binding-induced Sutured Toehold Activation for Controllable DNA Strand Displacement Reactions
Chemical Communications, 2015, 51, 2903-2906


42.Sensitive detection of transcription factors with Ag+-stabilized self-assembly triplex DNA molecular switch
Chemical Communications, 2014, 50, 14987-14990


41.Visualizing the endocytosis of phenylephrine in living cells by quantum dot-based tracking
Biomaterials, 2014, 35, 7042-7049


40.Quantitative detection of tumor necrosis factor-α by single molecule counting based on hybridization chain reaction
Biosensors and Bioelectronics, 2014, 60, 180-184


39.Toehold-mediated Strand Displacement Reaction Triggered Isothermal DNA Amplification for Highly Sensitive and Selective Fluorescent Detection of Single-base Mutation
Biosensors and Bioelectronics, 2014, 59, 276-281


38.Cascade Signal Amplification Strategy for Sensitive and Label-free DNA Detection Based on Exo III-catalyzed Recycling Coupled with Rolling Circle Amplification
Analyst, 2014, 139, 2884-2889


37.Aptamer-Based Exonuclease Protection and Enzymatic Recycling Cleavage Amplification Homogeneous Assay for Highly sensitive Detection of thrombin
Analyst, 2014, 139, 3167-3173


36.Quantitative Single-molecule Detection of Protein Based on DNA Tetrahedron Fluorescent Nanolabels
Talanta, 2014, 125, 393-399


35.An ultrasensitive fluorescence assay for protein detection by hybridization chain reaction-based DNA nanotags
Biosensors and Bioelectronics, 2014, 51, 421-425


34.Surface-enhanced Raman spectroscopy detection of polybrominated diphenylethers using a portable Raman spectrometer
Talanta, 2013, 116, 14-17


33.Cysteamine-Modified Silver Nanoparticle Aggregates for Quantitative SERS Sensing of Pentachlorophenol with a Portable Raman Spectrometer
ACS Appl. Mater. Interfaces, 2013, 5 (15), 6902-6908


32.Cysteine-Modulated Colorimetric Sensing of Extracellular Mg2+ in Rat Brain Based on the Strong Chelation Interaction between Dithiothreitol and Mg2+
Analyst, 2013,138, 3046-3052


31.A novel label-free cascade amplification strategy based on dumbbell probe-mediated rolling circle amplification-responsive G-quadruplex formation for highly sensitive and selective detection of NAD+ or ATP
Chemical Communications, 2013, 49, 2640-2642


30.A Novel Enzyme-free and Label-free Fluorescence Aptasensor for Amplified Detection of Adenosine
Biosensors and Bioelectronics, 2013, 44, 52-56


29.Selective and Sensitive Mercuric (II) Ion Detection Based on Quantum Dots and Nicking Endonuclease Assisted Signal Amplification
Biosensors and Bioelectronics, 2013, 43, 84-87


28.Electrospun porous CuO-Ag nanofibers for quantitative sensitive SERS detection
CrystEngComm, 2013, 15, 1339-1344


27.Visualization the Intracellular Location and Stability of DNA origami with a Label-free Fluorescent Probe
Chemical Communications, 2012, 48, 11301-11303


26.Quantitative Detection of Single DNA Molecules on DNA Tetrahedron Decorated Substrates
Chemical Communications, 2012, 48, 9661-9663


25.In situ fabrication of single-crystalline porous ZnO nanoplates on zinc foil to support silver nanoparticles as a stable SERS substrate
CrystEngComm, 2012, 14, 6023-6028


24.UV Photolysis-induced Generation of Graphene/M (M=Ag/Au) Hybrid Nanomaterials
Materials Letters, 2012, 84, 120-123


23.Silver nanoparticle aggregates on copper foil for reliable quantitative SERS analtsis of polycyclic aromatic hydrocarbons with a portable Raman spectrometer
Analyst, 2012, 137, 3995-4000


22.Micrococcal Nuclease Detection Based on Peptide-Bridged Energy Transfer between Quantum Dots and Dye-Labeled DNA
Talanta, 2012, 97, 533-538


21.Sensitive Detection of Proteins Using Assembled Cascade Fluorescent DNA Nanotags Based on Rolling Circle Amplication
Bioconjugate Chemistry, 2012, 23, 734-739


20.Strong Interaction between Imidazolium-Based Polycationic Polymer and Ferricyanide: toward Redox Potential Regulation for Selective In Vivo Electrochemical Measurements
Analytical Chemistry, 2012, 84, 1900-1906


19.A versatile platform for highly sensitive detection of protein: DNA enriching magnetic nanoparticles based rolling circle amplication immunoassay
Chemical Communications, 2012, 33, 3930-3932. Inside cover paper


18.Synthesis of a quinazoline derivative: A new a 1-adrenoceptors ligand for conjugation to quantum dots to study a 1-adrenoceptors in living cells
Bioorganic & Medicinal Chemistry Letters, 2011, 21, 5905-5909


17.Label free DNA detection based on gold nanoparticles quenching fluorescence of Rhodamine B
Talanta, 2011, 85, 725-729


16.A Sensitive Enzymatic Method for Paraoxon Detection based on Enzyme Inhibition and Fluorescence Quenching
Talanta, 2011, 84, 400-405


15.Quantitative Detection of Single Molecules Using Enhancement of Dye/DNA Conjugate-Labeled Nanoparticles
Bioconjugate Chemistry, 2010, 21, 1987-1993


14.Solid phase single-molecule counting of antibody binding to supported protein layers surface with low nonspecific adsorption
Talanta, 2010, 82, 1003-1009


13.Fluorescence single-molecule counting assays for protein quantification using epi-fluorescence microscopy with quantum dots labeling
Analytical Chimica Acta, 2010, 662, 170-176


12.DNA Quantification Based on FRET Realized by Combination with Surfactant CPB
Talanta, 2010, 81, 597-601


11.The co-luminescence effect of a europium(III)-lanthanum(III)-gatifloxacin-sodium dodecylbenzene sulfonate system and its application for the determination of trace amount of europium(III)
Journal of Luminescence, 2010, 130, 591-597


10.Quantitative detection of antibody based on single-molecule counting by total internal reflection fluorescence microscopy with quantum dot labeling
Analytica Chimica Acta, 2009, 634, 83-88


09.Rapid Determination of Minocycline in Pharmaceutical Formulations and Human Urine/Serum Samples Based on the Minocycline-Europium-Sodium Dodecylbenzene Sulfonate Luminescence System
Analytical Letters, 2009, 42, 228-242


08.Luminescence enhancement effect for the determination of balofloxacin with balofloxacin–europium (III)–sodium dodecylbenzene sulfonate system
Journal of Luminescence, 2009, 129, 90-94


07.Micelle-enhanced and terbium-sensitized spectrofluorimetric determination of gatifloxacin and its interaction mechanism
Spectrochimica Acta Part A, 2009, 72, 766-771


06.Rapid determination of gatifloxacin in biological samples and pharmaceutical products using europium-sensitized fluorescence spectrophotometry
Luminescence, 2008, 23, 7-13


05.Quantification of protein based on single-molecule counting by total internal reflection fluorescence microscopy with adsorption equilibrium
Analytica Chimica Acta, 2007, 590, 104-109


04.Fluorimetric Determination of Europium Based on the Co-fluorescence System Eu-Y-Sparfloxacin-SDS
Mikrochimica Acta, 2004, 144, 29-34


03.Determination of trace europium based on a new fluorescence system of europium-sparfloxacin-1,10-phenanthroline-sodium dodecyl sulfate
Analytical Bioanalytical Chemstry, 2003, 377(4), 681-684


02.Spectrofluorimetric determination of trace amounts terbium with 4-chlorsalicylic acid, EDTA, and cetyltrimethylammonium bromide
Analytical Sciences, 2003, 19, 923-925


01.Study of the absorption spectra of the 4f electron transitions of the praseodymium complex with ciprofloxacin and its analytical application
Analyst, 2001, 126, 1440-1443


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【科研项目】??



11、国家自然科学基金项目∶基于DNA催化自组装的模块化荧光生物传感技术及其生物医学应用研究，2015-2018，负责人




10、国家自然科学基金项目∶基于结构DNA纳米材料的生物单分子荧光成像技术研究，2012-2015，负责人



09、国家自然科学基金项目∶全内反射荧光显微术检测单个生物大分子，2008-2010，负责人


08、山东省自然科学基金重点项目∶量子点结合荧光共振能量转移技术检测生物大分子，2009-2011，负责人



07、山东省自然科学基金重点项目∶单分子计数及细胞内单分子运动轨迹的观测，2005-2007，负责人



06、国家自然科学基金项目∶组装DNA纳米结构用于肿瘤的早期诊断及协同载药，2014-2017，主要和作者



05、国家自然科学基金项目∶基于杂交链反应的单分子检测和高通量筛选，2012-2015，主要合作者


04、国家自然科学基金项目∶量子点示踪活细胞单分子检测，2009-2011，主要合作者



03、山东省自然科学基金重点项目∶量子点示踪实时区分单个活细胞中激动剂和拮抗剂，2006-2008，主要合作者


02、国家自然科学基金项目∶ 溶剂热合成超硬材料过程中物相控制新方法的探索，2005-2007，主要合作者


01、横向项目∶生物样品分析新方法，2005-2007，负责人







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【科研成果】
【所获专利】

【联合培养情况】
【拟招收研究生情况】??



本实验室瞄准生命分析化学的前沿科学问题，致力于生命分析新原理、新方法的研究，学术氛围浓厚，科研环境




优越，科研装备精良，科研经费充裕，科研水平上乘，科研成果丰硕。2016年拟招收博士研究生（含硕博连读研




究生）1-2名，硕士研究生（含推免生）2-3名，热忱欢迎富有创新精神，勇于探索的同学加盟，让我们一起探索




生命的奥秘！