报告题目：Crop Biotechnology–Trait Modification, Gene Containment, Gene Discovery and Genomic Tool Development in Perennial Grasses

　　报 告 人：Dr. Hong Luo

　　工作单位：Department of Genetics and Biochemistry, Clemson University, USA

　　报告时间：2015年12月1日（星期二）9:00-10:30

　　报告地点：行政楼205报告厅

　　报告人简介：

　　罗宏（Hong Luo）博士，美国克莱姆森大学（Clemson University）遗传及生物化学系终身教授。毕业于四川农业大学，分别于1983和1986年获得农学学士和植物遗传育种硕士学位。后赴比利时留学，于鲁汶大学获得分子生物学硕士和博士学位。罗宏博士先后在法国国家研究中心和美国普渡大学完成博士后研究并进入工业界工作。2006年罗宏博士返回学术界，从事植物DNA重组及转基因技术研发和能源草、牧草及草坪草遗传改良与分子设计研究。迄今发表论文60余篇，申请专利10余项。现为美国国家科学基金、美国农部及中国国家自然科学基金重点科研项目评审，也是30多个国际学术刊物审稿人，并担任克莱姆森大学遗传及生物化学专业研究生招生委员会主任，同时也是华中农业大学讲座教授。

　　代表性文章：

　　1. Yuan, S., Li, Z., Li, D., Hu, Q., Luo, H. (2015) Constitutive expression of Osa-miR528 alters plant development and enhances plant tolerance to salinity stress and nitrogen starvation in transgenic creeping bentgrass (Agrostis stolonifera L.). Plant Physiology 169:576-593.

　　2. Yuan, N., Cogill, S., Luo, H. (2015) Development of molecular strategies for gene containment and marker-free genetically modified organisms. In: Genetically Modified Organisms in Food. Watson, R., Stevens, B. (eds), Elservier B.V., pp223-236.

　　3. Yuan, S., Luo, H. (2015) Negative regulators of messenger RNA and the role of microRNA for plant genetic engineering. In: Genetically Modified Organisms in Food. Watson, R., Stevens, B. (eds), Elservier B.V., pp237-255.

　　4. San B., Li Z., Hu Q., Reighard G., Luo H. (2015) Adventitious shoot regeneration from in vitro cultured leaf explants of peach is significantly enhanced by silver thiosulfate. Plant Cell, Tissue and Organ Culture 120:757-765.

　　5. Zhou, M., Luo, H. (2014) Role of microRNA319 in creeping bentgrass salinity and drought stress respons. Plant signaling & Behavior 9:e28700.

　　6. Saski, C., Luo, H. (2014) Switchgrass genomic resources development and genome sequencing initiatives. In: Compendium of Bioenergy Plants – Switchgrass. Luo, H., and Wu, Y. (eds), CRC Press, Tailor & Francis Group, pp214-227.

　　7. Li, D., Zhou, M., Li, Z., Luo, H. (2014) MicroRNAs and their potential applications in switchgrass improvements. In: Compendium of Bioenergy Plants – Switchgrass. Luo, H., and Wu, Y. (eds), CRC Press, Tailor & Francis Group, pp228-252.

　　8. Zhou, M., Li, D., Li, Z., Hu, Q., Yang, C., Zhu, L., Luo, H. (2013) Constitutive expression of a miR319 gene alters plant development and enhances salt and drought tolerance in transgenic creeping bentgrass (Agrostis stolonifera L.). Plant Physiology 16:1375-1391.

　　9.Zhou, M., Luo, H. (2013) MicroRNA-mediated gene regulation: potential applications for plant genetic engineering. Plant Molecular Biology 83:59-75.

　　10. Li, Z., Hu, Q., Zhou, M., Vandenbrink, J., Li, D., Menchyk, N., Reighard, S.R., Norris, A., Liu, H., Sun, D., Luo, H. (2013) Heterologous expression of OsSIZ1, a rice SUMO E3 ligase enhances broad abiotic stress tolerance in transgenic creeping bentgrass. Plant Biotechnology Journal 11:432-445.

　　11. Li, Z., Zhou, M., Hu, Q., Reighard, S., Yuan, S., Yuan, N., San, B., Li, D., Jia, H. and Luo, H. (2012) Manipulating expression of tonoplast transporters. In: Plant Salt Tolerance: Methods and Protocols, Methods in Molecular Biology, vol. 913, DOI 10. 1007/978-1-61779-986-0_24, Shabala, S. Cuin, TA. (eds), Springer Science+Business Media, LLC, pp359-369.

　　12. Zhou M, Hu Q, Li Z, Chen C-F, Luo H (2011) Expression of a novel antimicrobial peptide penaeidin4-1 in creeping bentgrass (Agrostis stolonifera L.) enhances plant fungal disease resistance. PLoS ONE 6(9):e24677.

　　13. Saski CA, Li Z, Feltus FA, Luo H (2011) New genomic resources for switchgrass: a BAC library and comparative analysis of homoeologous genomic regions harboring bioenergy traits. BMC Genomics 12:369.

　　14. Fang G-C, Blackmon BP, Henry DC, Staton ME, Saski CA, Hodges SA, Tomkins JP, Luo H (2010) Genomic tools development for Aquilegia: Construction of a BAC-based physical map. BMC Genomics 11:621.

　　15. Li Z, Baldwin CM, Hu Q, Liu H, Luo H (2010) Heterologous expression of Arabidopsis H+-PPase enhances salt tolerance in transgenic creeping bentgrass (Agrostis stolonifera L.). Plant, Cell and Environment 33:272-289.

　　16. Ke PC, Lin S, Reppert J, Rao A, Luo H (2010) Uptake of nanoparticles by mammalian cells and plants, In: Handbook of Nanophysics - Nanomedicine and Nanorobotics, Sattler KD (ed), Taylor&Francis Group (CRC Press) Boca Raton, FL, pp30:1-15.

　　代表性专利:

　　1. Luo H, Hu Q, Nelson K, Longo C, Kausch AP, Zilinskas B, Lakkaraju S (2009) Prevention of transgene escape in genetically modified perennials. Patent No. US7,525,015 issued on April 28, 2009.

　　2. Luo H, Kausch AP, Chandlee JM, Oliver MJ (2005) Development of controlled total vegetative growth for prevention of transgene escape from genetically modified plants and for enhancing biomass production. USPTO application #20050235379.

　　3. Luo H (2007) Methods and compositions for an integrated dual site-specific recombination system for producing environmentally safe and clean transgenic plants. USPTO application #60/950,049.

　　4. Luo H, Zhou M, Hu Q (2009) Methods and compositions for transgenic plants producing antimicrobial peptides for enhanced disease resistance. USPTO application #61/247,103.

　　5. Luo H, Li Z, Hu Q (2010) Methods and compositions for transgenic plants with enhanced abiotic stress resistance and biomass production. USPTO application #61/302,345.

　　报告摘要：

　　Drought and salinity are already widespread in many regions, and are expected to cause serious salinization of more than 50% of all arable lands by the year 2050. They are therefore among the most important targets for improvement in plants. Using forward and reverse genetics approaches, we have been conducting research to identify and functionally characterize genes involved in various aspects of plant response to adverse environmental conditions. This allows the development of novel molecular strategies for use in genetically improving crop species for enhanced agricultural production. Perennial grasses are essential components of agriculture and environment, among which turfgrass, forages and biofuel plants play increasingly important roles in modern agriculture practice, significantly impacting agriculture structure, agriculture production, agriculture economy, environment, ecology and global climate. Genetic improvement of perennials using biotechnology approaches is important to the turfgrass industry, biofuel production and the environment. With the development of various gene containment strategies, it is expected that a combination of approaches stacking different containment measures could provide effective way to prevent transgene escape from transgenic perennials. However, it remains to see what the direct or indirect effects of the transgenes on host biochemistry, physiology, and consequently the potential impacts on non-target organisms and environmental and ecological systems would be? To address these questions, we are conducting research to genetically engineer creeping bentgrass for enhanced abiotic stress resistance using different mechanisms, Greenhouse and field trial evaluation of transgenic plants will allow to assess how transgenic perennials interact with other plant species, such as weeds and forages, how transgene expression impact weediness and invasiveness of genetically engineered perennials compared to unmodified parent organisms as well as what the effects of transgenic perennials on non-target soil chemistry would be.