翟诗翔^1,2,3, 李文军^1,3, 李莉莉^1,3, 王莉⁴, 秦松^1,3
1.中国科学院烟台海岸带研究所, 山东 烟台 264003;2.中国科学院大学, 北京 100049;3.中国科学院海洋大科学研究中心, 山东 青岛 266071;4.烟台市奇山医院, 山东 烟台 264001

摘要:
为开发藻类活性物质的药用价值，探究了藻蓝蛋白（phycocyanin，PC）对四氯化碳（CCl₄）诱导的大鼠肝纤维化的影响。试验中将大鼠随机分为3组，分别为对照组、肝纤维化组、CCl₄+PC干预组。利用CCl₄腹腔注射诱导肝损伤模型，在干预后，通过RT-PCR技术检测了大鼠肝脏中纤维化标志物I型胶原蛋白（collagen type I，Co-I）和α-平滑肌肌动蛋白（alpha-smooth muscle actin，α-SMA）的表达情况，通过16S rRNA高通量测序技术检测了干预后大鼠肠道微生物的组成和结构。RT-PCR结果显示PC干预显著降低了CCl₄诱导的大鼠肝脏中纤维化标志物α-SMA和Co-I的表达。CCl₄诱导使大鼠肠道菌群发生了紊乱，显著降低了具有抗炎活性的益生菌Bacteroides、Blautia、Parabacteroides的丰度，PC干预显著增加了具有抗炎活性的益生菌Blautia的丰度。结果表明PC能够改善CCl₄引起的肠道菌群紊乱，这种改善可能有助于缓解CCl₄导致的肝纤维化。
关键词:藻蓝蛋白肝纤维化肠道微生物炎症四氯化碳
DOI：10.11759/hykx20200314002
分类号:P375
基金项目:烟台市重点研发项目（2019XDHZ101；2020MSGY084）

Effects of phycocyanin on carbon tetrachloride-induced hepatic fibrosis and gut microbiota in rats
ZHAI Shi-xiang^1,2,3, LI Wen-jun^1,3, LI Li-li^1,3, WANG Li⁴, QIN Song^1,3
1.Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;4.Infectious Disease (Qishan) Hospital of Yantai, Yantai 264001, China
Abstract:
The effect of phycocyanin (PC) on carbon tetrachloride (CCl₄)-induced liver fibrosis and gut microbiota in rats were investigated to develop a medicinal value of algal active substances. Rats were randomly divided into three groups:control group (NC), hepatic fibrosis group (FIB), and PC + CCl₄intervention group (FIB_PC). After a 4-wk-long intervention, the messenger ribonucleic acid (mRNA) expression of α-smooth muscle actin (α-SMA) and collagen type I (Co-I) in rat liver were detected by real-time reverse transcription polymerase chain reaction, and composition of gut microbiota were detected by 16S rRNA high-throughput sequencing. Expression of α-SMA and Co-I in liver were significantly higher in FIB group than those in NC group. Expression of α-SMA and Co-I in the PC group was decreased after PC intervention. CCl₄ reduced the abundance of Bacteroides, Parabacteroides, and Blautia, which had anti-inflammatory activity. PC intervention significantly increased the abundance of Blautia, a probiotic with anti-inflammatory activity. These results indicated that PC could maintain gut microbiota homeostasis, which might alleviate liver fibrosis caused by CCl₄.
Key words:phycocyaninhepatic fibrosisgut microbiotainflammationcarbon tetrachloride





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