张小华^1,2,3, 刘东艳⁴
1.中国科学院烟台海岸带研究所 海岸环境过程重点实验室, 山东 烟台 264003;2.中国科学院大学, 北京 100049;3.滨州医学院 药学院, 山东 烟台 264003;4.华东师范大学 河口与海岸学国家重点实验室, 上海 200241

摘要:
为探讨温度与溶解态有机磷（Dissolved organic phosphorus，DOP）对浮游植物生长的共同影响，本文以中肋骨条藻为研究对象，选取含有C-O-P键的三磷酸腺苷（Adenosine triphosphate，ATP）、甘油磷酸钠（Sodium glycerophosphate，SG-P）、6-磷酸葡萄糖（Glucose 6-phosphate，G-6-P）和含有C-P键的草甘膦（Glyphosate，G-P）为不同类型的DOP，检测了4种温度条件下（20、23、26、29℃）藻细胞的生长状况以及培养液中总溶解态磷（Total dissolved phosphorus，TDP）、溶解态无机磷（Dissolved inorganic phosphorus，DIP）浓度与藻细胞内碱性磷酸酶（Alkaline phosphatase，AP）的变化。研究结果表明：含有C-O-P键的ATP、SG-P和G-6-P均能较好地维持中肋骨条藻的生长，并且升温可进一步促进各组藻细胞的生长，最大藻细胞密度分别达到176.7×10⁴、218.8×10⁴和178×10⁴ cell/mL；此外升温还可加速ATP、SG-P和G-6-P组培养液中TDP浓度的下降，以及ATP组培养液中DIP浓度的升高；但各组藻细胞内AP活性在不同温度条件下均处于较低水平（< 10 fmol pNP/（cell· h））。与含有C-O-P键的DOP相比，G-P组的藻细胞在各温度条件下生长密度均较低，最大藻细胞密度仅为40.7×10⁴ cell/mL；但其藻细胞内AP活性显著高于ATP、SG-P和G-6-P组[最高达83 fmol pNP/（cell·h）]且随温度升高而降低。以上结果表明，不同类型DOP会显著影响中肋骨条藻的生长，其中ATP、SG-P和G-6-P能更为有效地被中肋骨条藻生长所利用，并且温度升高（20~29℃）是促进3种DOP条件下中肋骨条藻生长的重要环境因素，但该效应可能并不依赖于AP活性。
关键词:中肋骨条藻温度生长溶解态有机磷溶解态无机磷碱性磷酸酶
DOI：10.11759/hykx20200225001
分类号:X173
基金项目:国家自然科学基金项目（41876127）；国家重点研发计划项目（2016YFE0133700，2016YFA0600904）

Effects of temperature and dissolved organic phosphorus on the growth characteristics of Skeletonema costatum
ZHANG Xiao-hua^1,2,3, LIU Dong-yan⁴
1.Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Department of pharmacy, Binzhou Medical University, Yantai 264003, China;4.State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200241, China
Abstract:
The effects of temperature and dissolved organic phosphorus (DOP) on the growth of phytoplankton were investigated in this study. We selected Skeletonema costatum as the study species and used adenosine triphosphate (ATP), sodium glycerophosphate (SG-P), glucose 6-phosphate (G-6-P) that contains the C-O-P ester bond, and glyphosate (G-P) that contains the C-P bond as DOP sources to measure cell density, total dissolved phosphorus (TDP), and dissolved inorganic phosphorus (DIP) concentrations in the culture medium and alkaline phosphatase (AP) activity in algal cells under four temperature conditions (i.e., 20℃, 23℃, 26℃, and 29℃). Results showed that ATP, SG-P, and G-6-P with a C-O-P ester bond could maintain the growth of Skeletonema costatum, and warming could promote the growth of algal cells in each group. The maximum cell concentrations in the ATP, SG-P, and G-6-P groups reached 176.7×10⁴, 218.8×10⁴, and 178×10⁴ cells/mL, respectively. Warming could also accelerate the decrease of TDP concentration in the ATP, SG-P, and G-6-P groups and the increase of DIP concentration in the ATP group. The AP activity in the algal cells of each group was low at different temperatures (<10 fmol pNP/(cell·h)). Compared with that in G-6-P with C-O-P ester bond, the algal cells in G-P with C-O-P bond were lower under the four temperature conditions with maximum cell concentration of 40.7×10⁴ cells/mL. However, the AP activity in the algal cells was higher than that in the ATP, SG-P, and G-6-P groups (up to 83 fmol pNP/(cell·h)) and decreased with warming. These results showed that different types of DOPs could significantly affect the growth of Skeletonema costatum, among which ATP, SG-P, and G-6-P could be more effectively used by algal cells. Moreover, warming (20~29℃) was an important environmental factor that promoted the growth of algal cells under ATP, SG-P, and G-6-P conditions but did not depend on the AP activity.
Key words:Skeletonema costatumtemperaturegrowthdissolved organic phosphorusdissolved inorganic phosphorusalkaline phosphatase





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