尹秀晶^1,2,3,4, 刘松^1,2,3, 秦玉坤^1,2,3, 邢荣娥^1,2,3, 于春林^1,2,3,4, 李克成^1,2,3, 李鹏程^1,2,3
1.中国科学院海洋研究所 实验海洋生物学重点实验室, 山东 青岛 266071;2.青岛海洋科学与技术试点国家实验室 海洋药物与生物制品功能实验室, 山东 青岛 266237;3.中国科学院 海洋大科学研究中心, 山东 青岛 266071;4.中国科学院大学, 北京 100049

摘要:
利用壳寡糖氨基与γ-氨基丁酸羧基发生酰胺化反应合成新型壳寡糖-氨基丁酸衍生物，并通过傅里叶变换红外光谱（FT-IR）、核磁共振光谱（NMR）等对合成的衍生物进行结构表征。以小麦种子为材料，各样品对小麦种子进行浸种处理后干旱胁迫培养到两叶一心，测定小麦生理生化指标，评价新型衍生物的抗旱作用。结果表明，新型壳寡糖γ-氨基丁酸衍生物成功合成，并且对干旱胁迫下小麦生理生化指标有明显的影响，衍生物明显降低了小麦幼苗叶片的丙二醛含量和细胞膜透性，缓解了干旱胁迫对小麦细胞膜的损伤，增加可溶性糖含量进而增强小麦植物对土壤中水分的吸收能力，减少了干旱胁迫对生理代谢的影响。总之，成功合成了具有较强诱导小麦抗旱作用的新型壳寡糖γ-氨基丁酸衍生物，为新型植物抗逆诱导剂的开发提供了思路。
关键词:壳寡糖γ-氨基丁酸抗旱
DOI：10.11759/hykx20200120005
分类号:Q945
基金项目:山东省重点研发项目（2018GHY115008，2019GHY112015，2018GHY115017）

Preparation of novel γ-aminobutyric acid-modified chitooligosaccharide and their effects on drought resistance of wheat seedlings
YIN Xiu-jing^1,2,3,4, LIU Song^1,2,3, QIN Yu-kun^1,2,3, XING Rong-e^1,2,3, YU Chun-lin^1,2,3,4, LI Ke-cheng^1,2,3, LI Peng-cheng^1,2,3
1.Key Laboratory of Experimental Marine Biology, titute of Oceanology of the Chinese Academy of Sciences, Qingdao 266071;2.Functional Laboratory of Marine Drugs and Biological Products, Pilot National Laboratory of Marine Science and Technology(Qingdao), Qingdao 266237, China;3.Center for Ocean Mega-science, Chinese Academy of Sciences, Qingdao 266071, China;4.University of Chinese Academy of Sciences, Beijing 100049, China
Abstract:
The novel γ-aminobutyric acid-modified chitooligosaccharide was synthesized by the amination of the amino group of the chitooligosaccharide with the carboxyl group of γ-aminobutyric acid, and its structure was characterized using Fourier-transform infrared and magnetic resonance spectra (NMR). In this paper, wheat seeds were soaked with chitooligosaccharide, γ-aminobutyric acid, and γ-aminobutyric acid-modified chitooligosaccharide. Their drought-resistant effects were evaluated by measuring the relevant indicators after they grew for a two-leave period under drought stress. The results showed that the novel derivatives were synthesized successfully and had positive effects on wheat under drought stress. The derivatives significantly reduced the malondialdehyde content and cell membrane permeability of wheat seedling leaves, alleviated the damage to the wheat cell membrane caused by drought stress, increased the soluble sugar content, and enhanced the soil water absorption capacity of wheat plants. Therefore, the newly synthesized γ-aminobutyric acid-modified chitooligosaccharide can significantly reduce the effects of drought stress on wheat metabolism. In conclusion, a novel chitooligosaccharide derivative with a robust drought-induced effect on wheat was successfully synthesized and might provide an idea for the development of a new plant stress-resistant inducer.
Key words:chitooligosaccharideaminobutyric aciddrought-resistant activity





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