李冬艳¹, 王晶¹, 陈科良², 朱虎成², 陈春梅², 张锦文¹
1.华中科技大学同济医学院附属同济医院药学部, 湖北 武汉 430030;2.华中科技大学同济医学院药学院, 湖北 武汉 430030

摘要:
为了研究烟曲霉次生代谢产物，我们采用硅胶柱层析、sephadex LH-20凝胶柱层析、半制备液相色谱等分离方法对来源于印度洋深海区的烟曲霉（Aspergillus fumigatus）的乙醇提取液进行系统的次生代谢产物分离，根据所分离得到的化合物的理化性质和波谱数据确定化合物的结构。共分离鉴定了15个化合物，其中11个二酮哌嗪类生物碱（diketopiperazine），4个喹唑啉类衍生物（fumiquinazoline），分别为：fumitremorgin B （1），13-oxofumitremorgin B （2），fumitremorgin C （3），12-hydroxyfumitremorgin C （4），cyclotyprostatins B （5），cyclotyprostatins C （6），cyclotyprostatins E （7），verruculogen （8），spirotryprostatin C （9），spirotryprostatin D （10），spirotryprostatin F （11），fumiquinazoline C （12），fumiquinazoline D （13），3-hydroxyfumiquinazoline A （14）和fumiquinazoline E （15）。分离得到的15个化合物，丰富了烟曲霉次生代谢产物的类型，为烟曲霉的开发利用提供了化学研究基础。
关键词:烟曲霉(Aspergillusfumigatus)曲霉属(Aspergillus)次生代谢产物化学成分波谱数据
DOI：10.11759/hykx20190411002
分类号:R914.4
基金项目:国家自然科学基金青年项目（31600266）

Study of the secondary metabolites of Aspergillus fumigatus from the deepsea zone of the Indian Ocean
LI Dong-yan¹, WANG Jing¹, CHEN Ke-liang², ZHU Hu-cheng², CHEN Chun-mei², ZHANG Jin-wen¹
1.Department of Pharmacy, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;2.Pharmaceutical college, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Abstract:
This study was conducted to analyze the chemical constituents of the secondary metabolites of Aspergillus fumigatus for which the chemical constituents of the ethanol extract of A. fumigatus from the deep sea zone of the Indian Ocean were separated systematically using silica gel column chromatography, Sephadex LH-20 column chromatography, and RP-HPLC. Physicochemical properties and spectral data were used to determine the structures of the isolated compounds. A total of 15 compounds were isolated that were identified as fumitremorgin B (1), 13-oxofumitremorgin B (2), fumitremorgin C (3), 12-hydroxyfumitremorgin C (4), cyclotyprostatins B (5), cyclotyprostatins C (6), cyclotyprostatins E (7), verruculogen (8), spirotryprostatin C (9), spirotryprostatin D (10), spirotryprostatin F (11), fumiquinazoline C (12), fumiquinazoline D (13), 3-hydroxyfumiquinazoline A (14), and fumiquinazoline E (15). These 15 compounds isolated from A. fumigatus enriched the type of the secondary metabolites of the fungus and provided a basis for exploring its development and conducting chemical research.
Key words:Aspergillus fumigatusAspergillusthe secondary metaboliteschemical constituentsspectral data





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