周胜杰^1,2, 胡 静^1,2, 于 刚^1,2, 杨其彬¹, 杨 蕊¹, 刘亚娟^1,3, 马振华^1,2
1.中国水产科学研究院 南海水产研究所 热带水产研究开发中心;2.农业部南海渔业资源开发利用重点试验室;3.天津农学院

摘要:
为研究不同光照时长对尖吻鲈(Lates calcarifer)消化酶活力的影响, 作者采用生物化学方法系统测定不同光周期(光照时长分别为13、16、19 和24 h)下, 对尖吻鲈仔鱼阶段和稚鱼阶段3种消化酶(胃蛋白酶PP、淀粉酶AMS、脂肪酶LPS)活性变化进行对比研究。结果表明, 16 h光照时长PP的活力最高, 过长的光照时长会抑制PP的活性; 增加光照时长对AMS的活性有抑制作用, 而对于LPS的活性呈现先抑制后促进的现象。在24 h光照时长时LPS 活性增加; 在19 h光照时长时对PP活性抑制; 对淀粉酶影响不明显。16 h光照时长能有效提高尖吻鲈仔、稚鱼的生长速度, 更长的光照时长反而抑制其生长。研究表明, 不同光照周期对尖吻鲈同种消化酶的影响不同; 同一光照周期对不同消化酶产生的影响不同; 尖吻鲈稚鱼阶段消化酶对光照时长变化的反应小于仔鱼期, 适当地延长光照时长有助于提高尖吻鲈的生长速度。研究结果有助于选择特定的光周期促进某消化酶的活性, 达到提高对特定营养物质的消化吸收能力, 提高生产实践中仔、稚鱼的生长速度。
关键词:尖吻鲈(Lates calcarifer)消化酶光周期酶活
DOI：10.11759/hykx20180201003
分类号:
基金项目:中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2017ZD01; 2018ZD01); 海南省重点研发计划(ZDYF2017036, ZDYF2018096)

Effects of photoperiod on digestive enzyme activity in larval and juvenile barramundi Lates calcarifer (Bloch)
ZHOU Sheng-jie,HU Jing,YU Gang,YANG Qi-bin,YANG Rui,LIU Ya-juan,MA Zhen-hua
Abstract:
The activity of digestive enzymes can respond to the digestive ability of the larynboster (Lates calcarifer) to different nutrient components. When a certain condition changes, the digestive enzyme activity changes, so studying the effect of different light duration on the activity of digestive enzymes is necessary to understand The nature of digestive enzymes is of great significance. The study showed that prolonged light time had a great influence on the digestive enzyme activity of the larvae. The maximum activity of PP was in 16 h group, but longer photoperiod inhibited the activity of PP. The activity of AMS was inhibited by increasing the light time, while the activity of LPS was inhibited firstly and then promoted. The increase of light time showed little effect on the digestive enzyme activity of the juvenile fish. But specific light cycles have different effects on different digestive enzymes (promote or inhibit); LPS activity increased in 24 h light hours group; PP was inhibited during the illumination of 19 h group; The effect of amylase AMS was not obvious. The results showed that different light cycles had different effects on digestive enzymes. The digestive enzymes of different growth stages have different reactions to the same light. The results of the study can help to select the specific light cycle to promote the activity of certain digestive enzymes and to improve the digestion and absorption capacity of certain nutrients.
Key words:barramundidigestive enzymesphotoperiodenzyme activity





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