DOI: 10.11908/j.issn.0253-374x.2018.07.012

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Screening and Characterization of an Efficient Phthalate-Ester-Degrading Strain
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ÕªÒª:ÒÔÁÚ±½¶þ¼×Ëá¶þ¶¡õ¥£¨DBP£©ÎªÎ¨Ò»Ì¼Ô´£¬É¸Ñ¡µÃµ½Ò»ÖêÄܹ»½µ½âDBPµÄ¾úÖê.ͨ¹ýÐÎ̬ѧ¹Û²ì¡¢16S rDNA²âÐò¼°ÏµÍ³·¢Óý·ÖÎö£¬¼ø¶¨¸Ã¾úÖêΪ¹ÑÑøµ¥°û¾ú£¨Stenotrophomonas sp.£©£¬ÃüÃûΪB3.Ê״ᨵÀÁ˹ÑÑøµ¥°û¾ú¶ÔÁÚ±½¶þ¼×Ëáõ¥(PAEs)µÄ½µ½â×÷ÓÃ.B3¿É¸ßЧ½µ½â0.05~50 g?L-1Ũ¶È·¶Î§µÄDBP£¬ÆäÖжÔ0.05 g?L-1DBPµÄ½µ½âÂÊΪ93.4%.¾­¹ýÓÅ»¯ºó£¬ÔÚ35 ¡æ¡¢pH 8 Ìõ¼þ϶Ô10 g?L-1DBPµÄ½µ½âÂÊΪ95.8%.·´Ó¦¶¯Á¦Ñ§Ñо¿±íÃ÷£¬B3½µ½âDBP·ûºÏÒ»¼¶½µ½â¶¯Á¦Ñ§Ä£ÐÍ£¬¶Ô50 g?L-1DBP³õʼ½µ½âËÙÂÊ¿É´ï1.25 g?L-1?h-1.B3¶ÔÆäËû4ÖÖ³£¼ûµÄPAEs£¨ÁÚ±½¶þ¼×Ëá¶þ£¨2©¤ÒÒ»ù¼º£©õ¥¡¢ÁÚ±½¶þ¼×Ëá¶þ¼×õ¥¡¢ÁÚ±½¶þ¼×ËᶡÜÐõ¥¡¢ÁÚ±½¶þ¼×Ëá¶þÒÒõ¥£©ºÍ±½°·¡¢¼×±½¡¢ÁÚ±½¶þ¼×ËáµÄ½µ½âÂʾùÔÚ50%¼°ÒÔÉÏ.B3½µ½âPAEsŨ¶È·¶Î§¿í¡¢µ×ÎïÖÖÀ෶Χ¹ã£¬±íÃ÷B3ÔÚPAEsµÄÉúÎï½µ½âÖоßÓÐÁ¼ºÃµÄÓ¦ÓÃÇ°¾°.



Abstract:With dibutyl phthalate (DBP) as the sole carbon source, we isolated a DBPª²degrading strain; by its morphology as well as 16S rDNA sequencing and phylogenetic analysis, the strain was identified as Stenotrophomonas sp., designated as strain B3. Degradation of Phthalate esters (PAEs) by Stenotrophomonas was reported for the first time. The degradation efficiency of 0.05 g?L-1 DBP is 93.4%;B3 could efficiently degrade DBP up to 50 g?L-1,and under the conditions of pH 8, 35 ¡æ, the degradation efficiency of 10 g?L-1 DBP by B3 reached 95.8%. Kinetic studies showed that DBP degradation by B3 followed first-order kinetics, and the initial degradation rate of 50 g?L-1 DBP can reach 1.25 g?L-1?h-1. The degradation efficiencies of four other common PAEs£¨di-2-ethylhexylPhthalate¡¢dimethyl phthalate¡¢benzyl butyl phthalate¡¢diethyl phthalate£©as well as aniline, toluene and phthalic acid by B3 were 50% or higher, suggesting that B3 had a broad substrate spectrum. The ability of B3 to degrade various PAEs at high concentrations indicates that B3 is promising in bioremediation of PAEs.





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