张颖莹 讲师，主治医师
硕士招生类型：学术型
硕士招生专业：内科学（肾内科学）
博士招生类型：无
博士招生专业：无
所在院系：附属同济医院
联系方式：idklaa@126.com
课题组链接：
研究方向
主要任职
科研业绩

非编码RNA与肾脏纤维化；Non-coding RNA and Kidney Fibrosis
上海市医学会肾脏病分会 青年委员；中国生理协会肾脏病分会青年委员 Shanghai Medical Association of Nephrology， youth committee member；Chinese Association for Physiological Sciences of Nephrology, youth committee member
张颖莹，博士，主治医师，上海市医师协会肾脏病分会青年委员， 中国生理协会肾脏病分会青年委员。本人主要从事的领域为 Smad3 相关的长链非编 码 RNA 在肾脏纤维化中的机制研究。近3年发表 SCI 论文数10余篇， 累积影响因子超过50分；申请人目前承担课题 4 项，参与课题 数 项，其中主持国家自然科学基金青年项目1项及中华医学会发展基金项目 1 项。
过去 5 年中，从事的研究集中在长链非编码 RNA（lncRNA）在肾脏病中的研究。肾脏纤维化是所有急慢性肾脏疾病的终末期共同通路，长链非编码 RNA 由于其独特的组织及器官特异性为近些年的研究热点。首先我们在小鼠模型中通过 RNA-sequence 筛选找出差异性 lncRNA ；其次将筛选出lncRNAs进行全序列扩增，基因定位，证实其在真实世界的存在性，同时研究其在肾脏中的功能及作用机制。研究结果发现 LRNA9884通过 MCP-1 招募巨噬细胞促进糖尿病肾病的进展（该研究结果已发表在2019.08 Diabetes 杂志），我们进一步研究发现 LRNA9884与炎症密切相关，而急性肾损伤中炎症起着重要的作用。因此，在自然基金项目的支持下我们对LRNA9884在急性肾损伤进一步研究，结果提示急性肾损伤中，LRNA9884 通过调控MIF促进炎症，该研究2020.10被Front Physiol杂志接收。
前期研究中首先发现了 LRNA9884，并对其功能及机制进行鉴定，该研究为国际领先水平。但是针对 LRNA9884 的研究仍然存在一些不足，其中最大的困难为我们始终无法在人种属找到 LRNA9884 相关基因定位。下一步研究的GAS5为另一lncRNA，其兼具鼠源及人源种属的优势使我们既可以对其进行机制探究，同时可进一步实现转化医学。我们将在后续的研究中继续针对肾脏纤维化的机制进行研究。
The applicant, Dr. Zhang, currently is an attending physician, who is also a young member of The Kidney Disease Branch of Shanghai Medical Doctors Association and a young member of the Kidney Disease Branch of China Physiological Association. Her main field of study is the mechanism of Smad3-related long non-RNA in renal fibrosis. She has published more than 10 SCI papers in the recent three years, with accumulated impact factors of over 50 points. At present, the applicant has presided 4 projects and participated in several projects. One of the 2 projects she has presided over is national Natural Science Foundation for Youth and the other is Chinese Medical Association Development Fund.
In the past 5 years, Dr. Zhang’s focused on the role of long non-coding RNA (lncRNA) in renal fibrosis. Renal fibrosis is the common end-stage pathway of all kidney diseases. First, she identified differential lncRNAs in the mouse model by RNA-Sequence. Secondly, she identified the full sequence of LRNA9884 by using RACE, which hasn’t been reported previously. In this study, LRNA9884 was found to promote the progression of diabetic nephropathy by recruiting macrophages through MCP-1-dependent (date been published in 2019.08 Diabetes Journal). Her further study found that LRNA9884 was closely related to inflammation, which plays an important role in acute kidney injury as well. Therefore, she investigated LRNA9884 in acute kidney injury with the support of the Nature Physiotherapy Project. The results suggested that LRNA9884 promoted inflammation by regulating MIF in acute kidney injury. The study was accepted by Front Physiol in 2020, Oct. Nowadays, we concentrate on the lncRNAs being as a biomarker in clinical work.