学位：博士
教职：博士生导师
研究领域：干细胞与细胞重编程
E-mail:chenjiayu@tongji.edu.cn
联系电话：
实验室网址：http://gaolab.tongji.edu.cn
招生要求：无论你毕业自哪所学校，哪个专业，关键是你是否努力、认真、敢于尝试、不怕失败并且愿意并能够团队合作。我期待你能融入我们实验室的大家庭，互相帮助，探讨科研，为追求真理、探寻真相，不断努力。
研究方向：1.细胞命运转变过程中的表观遗传调控机制研究
2.基于CRISPR/Cas9基因编辑技术的疾病模型构建的研究
教育及工作经历：
2017.12-至今同济大学生命科学与技术学院，副教授
2014.10-2017.11同济大学生命科学与技术学院，助理教授
2014.2-2014.10 同济大学转化医学高等研究院，助理研究员
2009.9-2014.1 北京协和医学院，北京生命科学研究所，生物化学与分子生物学博士
2004.9-2009.6 复旦大学，上海医学院，医学学士
个人简介：
陈嘉瑜博士是同济大学生命科学与技术学院，高绍荣课题组的副教授，上海市晨光计划项目获得者(2016)，上海市青年科技启明星（A类）获得者(2018)，上海市人才发展资金资助计划获得者（2018），中国科协青年人才托举项目获得者（2018）。
陈嘉瑜博士主要进行细胞命运转变过程中的表观遗传调控机制研究，以及基于CRISPR/Cas9基因编辑技术的疾病模型构建的研究。首次证明了DNA羟甲基化酶Tet1及DNA羟甲基化调控细胞重编程的机制及其对细胞多能性的影响，研究成果在Cell Stem Cell杂志以封面研究发表，并被评为2013年度最佳论文之一。2018年，合作揭示了Stella参与卵子发生过程中DNA甲基化模式建立的机制，并首次在体内证实了DNMT1可以作为起始性DNA甲基化转移酶，打破了教科书里关于DNMT1只是维持性DNA甲基化转移酶的论断，研究成果发表在Nature杂志。此外，陈嘉瑜博士以主要骨干身份参与科技部“国家重点研发计划干细胞及转化研究重点专项”及“国家重大科学研究计划（973）”各一项，主持国家自然科学基金“面上项目”一项，结题国家自然科学基金“青年项目”一项。近几年，陈嘉瑜博士在Nature，Cell Stem Cell，Cell Research和Stem Cell Reports等国际知名学术期刊发表相关成果30余篇（SCI），其中第一作者（含共同）或通讯作者论文（含共同）共计12篇。此外，陈嘉瑜博士已培养或合作培养的3位硕士和4位博士研究生获得研究生国家或校级奖学金，带领本科生获得全国或国际生命科学相关学科竞赛奖项的多位硕士或博士研究生获得研究生国家或校级奖学金。
发表论文：
1.He W#, Chen J#*, Gao S*. Mammalian haploid stem cells: establishment, engineering and applications. Cell Mol Life Sci. 2019 Jun;76(12):2349-2367. doi: 10.1007/s00018-019-03069-6. Epub 2019 Mar 19. (#co-first authors, *Co-correspondence)
说明：陈嘉瑜副教授与何文腾博士在有近80年历史的欧洲老牌杂志《Cellular and Molecular Life Sciences》受邀发表单倍体胚胎干细胞相关综述。
2.Li Y#, Zhang Z#, Chen J#, Liu W, Lai W, Liu B, Li X, Liu L, Xu S, Dong Q, Wang M, Duan X, Tan J, Zheng Y, Zhang P, Fan G, Wong J, Xu G, Wang Z, Wang H, Gao S, Zhu B*. Stella safeguards the oocyte methylome by preventing de novo methylation mediated by DNMT1. Nature. 2018 Dec;564(7734):136-140. doi: 10.1038/s41586-018-0751-5. Epub 2018 Nov 28. (#co-first authors)
说明：陈嘉瑜副教授与中科院生物物理所朱冰研究员课题组合作，在《Nature》杂志发表论文，揭示卵子发生过程中DNA甲基化模式建立的机制，报道了Stella通过抑制DNMT1介导的起始性DNA甲基化作用，打破了教科书里关于DNMT1只是维持性DNA甲基化转移酶的论断。
3.Gao H#, Gao R#, Zhang L#, Xiu W, Zang R, Wang H, Zhang Y, Chen J*, Gao Y*, Gao S*. Esrrb plays important roles in maintaining self-renewal of trophoblast stem cells (TSCs) and reprogramming somatic cells to induced TSCs. Journal of Molecular Cell Biology. 2019 Jun 1;11(6):463-473. doi: 10.1093/jmcb/mjy054. (*Co-correspondence)
说明：该研究揭示了Esrrb在滋养层干细胞维持及自我更新中的重要作用以及对小鼠成纤维细胞向诱导型滋养层干细胞转化过程的促进作用。陈嘉瑜副教授为本文共同通讯作者。高海波博士、高睿博士后及张林凤博士研究生为本文的共同第一作者。
4.康岚#，陈嘉瑜#，高绍荣*。中国细胞重编程和多能干细胞研究进展。遗传, 2018, 40 (10): 825-840. doi: 10.16288/j.yczz.18-209
5.Li J#, Shen S#, Chen J#, Liu W, Li X, Zhu Q, Wang B, Chen X, Wu L, Wang M, Gu L, Wang H, Yin J, Jiang C*, Gao S*. Accurate annotation of accessible chromatin in mouse and human primordial germ cells. Cell Research. 2018 Nov;28(11):1077-1089. doi: 10.1038/s41422-018-0096-5. Epub 2018 Oct 10. (#co-first authors)
说明：本研究利用最新优化的low-input DNase-seq技术结合生物信息学大数据分析，精确注释了原始生殖细胞（PGC）发育过程中染色质的开放状态，为PGC发育过程中各个重要生物学事件提供了重要线索。陈嘉瑜副教授、李静一博士和沈仕君博士为本文共同第一作者。
6.He W#, Zhang X#, Zhang Y, Zheng W, Xiong Z, Hu X, Wang M, Zhang L, Zhao K, Qiao Z, Lai W, Lv C, Kou X, Zhao Y, Yin J, Liu W, Jiang Y, Chen M, Xu R, Le R, Li C, Wang H, Wan X, Wang H, Han Z, Jiang C*, Gao S*, Chen J*. Reduced Self-Diploidization and Improved Survival of Semi-cloned Mice Produced from Androgenetic Haploid Embryonic Stem Cells through Overexpression of Dnmt3b. Stem Cell Reports.2018 Feb 13;10(2):477-493. doi: 10.1016/j.stemcr.2017.12.024. Epub 2018 Jan 27. (*Co-correspondence)
说明：本研究揭示了Dnmt3b能恢复孤雄单倍体胚胎干细胞全基因组甲基化水平，延缓单倍体细胞的自发二倍体化，并提高半克隆小鼠发育潜能及存活能力。陈嘉瑜博士为本文共同通讯作者，何文腾博士为共同第一作者。
7. Liu W#, Bai D#, Chen J*, Gao S*. Additive-effect pattern of both ZP2 and ZP3 in human and mouse. Hum Genet.2017 Nov 1. doi: 10.1007/s00439-017-1848-x. (*Co-correspondence)
8. Liu W#, Li K#, Bai D#, Yin J#, Tang Y, Chi F, Zhang L, Wang Y, Pan J, Liang S, Guo Y, Ruan J, Kou X, Zhao Y, Wang H, Chen J*, Teng X*, Gao S*. Dosage effects of ZP2 and ZP3 heterozygous mutations cause human infertility. Hum Genet. 2017 Aug;136(8):975-985. doi: 10.1007/s00439-017-1822-7. Epub 2017 Jun 24.(*Co-correspondence)
说明：本研究首次报道了由ZP2、ZP3双杂合突变导致女性不育的病例，并通过动物及细胞模型阐明了其致病机制，患者经过干预，最终成功受孕并产下健康男婴。陈嘉瑜副教授为本文的共同通讯作者。
9.Gao R#, Xiu W#, Zhang L, Zang R, Yang L, Wang C, Wang M, Wang M, Yi L, Tang Y, Gao Y, Wang H, Xi J, Liu W, Wang Y, Wen X, Yu Y, Zhang Y, Chen L*, Chen J*, Gao S*. Direct induction of neural progenitor cells transiently passes through a partially reprogrammed state. Biomaterials.2017 Mar;119:53-67. doi: 10.1016/j.biomaterials.2016.12.007. (*Co-correspondence)
说明：本研究建立了一种以生长因子为基础的细胞转分化手段，发现其是一个渐进变化的过程，包括起始状态，中间状态，成熟状态以及稳定状态。相应的基因调控网络在这两个过程中均由体细胞特异性的调控网络向神经前体细胞特异性的调控网络靠拢。陈嘉瑜副教授为本文的共同通讯作者。
10.Xu K#, Chen X#, Yang H, Xu Y, He Y, Wang C, Huang H, Liu B, Liu W, Li J, Kou X, Zhao Y, Zhao K, Zhang L, Hou Z, Wang H, Wang H, Li J, Fan H, Wang F, Gao Y, Zhang Y,Chen J*, Gao S*. Maternal Sall4 Is Indispensable for Epigenetic Maturation of Mouse Oocytes. J Biol Chem.2017 Feb 3;292(5):1798-1807. doi: 10.1074/jbc.M116.767061. Epub 2016 Dec 28. (*Co-correspondence)
说明：本研究阐释了SALL4作为重要的转录因子和表观遗传调控因子参与卵母细胞成熟的调控机制。陈嘉瑜副教授为本文的共同通讯作者。
11.Chen J#*, Gao Y#, Huang H, Xu K, Chen X, Jiang Y, Li H, Gao S, Tao Y, Wang H, Zhang Y, Wang H, Cai T, Gao S*. The combination of Tet1 with Oct4 generates high-quality mouse induced pluripotent stem cells (iPSCs). Stem Cells.2015 Mar;33(3):686-98. doi: 10.1002/stem.1879. (#co-first authors, *Co-correspondence)
12.Liu X#, Chen J#, Liu W, Li X, Chen Q, Liu T, Gao S*, Deng M*. The fused in sarcoma protein forms cytoplasmic aggregates in motor neurons derived from integration-free induced pluripotent stem cells generated from a patient with familial amyotrophic lateral sclerosis carrying the FUS-P525L mutation. Neurogenetics. 2015 Jul;16(3):223-31. doi: 10.1007/s10048-015-0448-y. Epub 2015 Apr 26. (#co-first authors)
13.Gao Y#, Chen J#, Li K, Wu T, Huang B, Liu W, Kou X, Zhang Y, Huang H, Jiang Y, Yao C, Liu X, Lu Z, Xu Z, Kang L, Chen J, Wang H, Cai T and Gao S. Replacement of Oct4 by Tet1 during iPSC induction reveals an important role of DNA methylation and hydroxymethylation in reprogramming. Cell Stem Cell, 12(4):453-469, 2013.(Cover Story; Previewed by Cell Stem Cell; Selected as Best of 2013 by Cell Stem Cell)(#co-first authors)
说明：该研究首次证明了DNA羟甲基化酶Tet1及DNA羟甲基化调控细胞重编程的机制及其对细胞多能性的影响，研究成果在Cell Stem Cell杂志以封面研究发表，并被评为2013年度最佳论文之一。陈嘉瑜副教授为本文共同第一作者。
14. Zheng R#, Li Z#, He F#, Liu H*, Chen J, Chen J, Xie X, Zhou J, Chen H, Wu X, Wu J, Chen B, Liu Y, Cui H, Fan L, Sha W, Liu Y, Wang J, Huang X, Zhang L, Xu F, Wang J, Feng Y, Qin L, Yang H, Liu Z, Cui Z, Liu F, Chen X, Gao S, Sun S*, Shi Y*, Ge B*. Genome-wide association study identifies two risk loci for tuberculosis in Han Chinese. Nat Commun. 2018 Oct 4;9(1):4072. doi: 10.1038/s41467-018-06539-w.
15.Gao R#, Wang C#, Gao Y#, Xiu W, Chen J, Kou X, Zhao Y, Liao Y, Bai D, Qiao Z, Yang L, Wang M, Zang R, Liu X, Jia Y, Li Y, Zhang Y, Yin J, Wang H, Wan X, Liu W*, Zhang Y*, Gao S*. Inhibition of Aberrant DNA Re-methylation Improves Post-implantation Development of Somatic Cell Nuclear Transfer Embryos. Cell Stem Cell. 2018 Sep 6;23(3):426-435.e5. doi: 10.1016/j.stem.2018.07.017. Epub 2018 Aug 23.
16. Guo X#, Xu Y#, Wang Z, Wu Y, Chen J, Wang G, Lu C, Jia W, Xi J, Zhu S, Jiapaer Z, Wan X, Liu Z, Gao S, Kang J*. A Linc1405/Eomes Complex Promotes Cardiac Mesoderm Specification and Cardiogenesis. Cell Stem Cell.2018 Jun 1;22(6):893-908.e6. doi: 10.1016/j.stem.2018.04.013. Epub 2018 May 10.
17.Fang L#, Teng H#, Wang Y#, Liao G#, Weng L, Li Y, Wang X, Jin J, Jiao C, Chen L, Peng X, Chen J, Yang Y, Fang H, Han D, Li C, Jin X, Zhang S, Liu Z, Liu M, Wei Q, Liao L, Ge X, Zhao B, Zhou D, Qin HL, Zhou J, Wang P. SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis. Cancer Cell.2018 Jul 9;34(1):103-118.e9. doi: 10.1016/j.ccell.2018.06.002. Epub 2018 Jun 28
18.Wang C#, Liu X#, Gao Y#, Yang L#, Li C, Liu W ,Chen C, Kou X, Zhao Y, Chen J, Wang Y, Le R, Wang H, Duan T, Zhang Y, Gao S.(2018) Reprogramming of H3K9me3-dependent heterochromatin during mammalian embryo development. Nature Cell Biology. 2018 May;20(5):620-631. doi: 10.1038/s41556-018-0093-4. Epub 2018 Apr 23
19.Zhuang Q, Li W, Benda C, Huang Z, Ahmed T, Liu P, Guo X, Iba?ez DP, Luo Z, Zhang M, Abdul MM, Yang Z, Yang J, Huang Y, Zhang H, Huang D, Zhou J, Zhong X, Zhu X, Fu X, Fan W, Liu Y, Xu Y, Ward C, Khan MJ, Kanwal S, Mirza B, Tortorella MD, Tse HF, Chen J, Qin B, Bao X, Gao S, Hutchins AP, Esteban MA. NCoR/SMRT co-repressors cooperate with c-MYC to create an epigenetic barrier to somatic cell reprogramming. Nat Cell Biol.2018 Mar 12. doi: 10.1038/s41556-018-0047-x.
20.Zhu J, Wang K, Li T, Chen J, Xie D, Chang X, Yao J, Wu J, Zhou Q, Jia Y, Duan T. Hypoxia-induced TET1 facilitates trophoblast cell migration and invasion through HIF1α signaling pathway.Sci Rep.2017 Aug 14;7(1):8077. doi: 10.1038/s41598-017-07560-7.
21. Li Y, Hai Y, Chen J, Liu T. Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells.J Vis Exp. 2017 Jul 18;(125). doi: 10.3791/55722.
22.Li H, Lai P, Jia J, Song Y, Xia Q, Huang K, He N, Ping W, Chen J, Yang Z, Li J, Yao M, Dong X, Zhao J, Hou C, Esteban MA, Gao S, Pei D, Hutchins AP, Yao H. RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions. Cell Stem Cell.2017 Jan 11. pii: S1934-5909(16)30458-1. doi: 10.1016/j.stem.2016.12.002.
23.Liu X, Wang C, Liu W, Li J, Li C, Kou X, Chen J, Zhao Y, Gao H, Wang H, Zhang Y, Gao Y, Gao S (2016). Distinct features of H3K4me3 and H3K27me3 chromatin domains in pre-implantation embryos. Nature.2016 Sep 14;537(7621):558-562. doi: 10.1038/nature19362.
24. Xiong J, Zhang Z,Chen J, Huang H, Xu Y, Ding X, Zheng Y, Nishinakamura R, Xu G, Wang H, Chen S, Gao S and Zhu B (2016). Cooperative Action between SALL4A and TET Proteins in Stepwise Oxidation of 5-Methylcytosine. Molecular Cell, http://dx.doi.org/10.1016/j.molcel.2016.10.013.
25.Zhang W, Xia W, Wang Q, Towers AJ, Chen J, Gao R, Zhang Y, Yen CA, Lee AY, Li Y, Zhou C, Liu K, Zhang J, Gu TP, Chen X, Chang Z, Leung D, Gao S, Jiang YH, Xie W. (2016). Isoform Switch of TET1 Regulates DNA Demethylation and Mouse Development. Molecular Cell. http://dx.doi.org/10.1016/j.molcel.2016.10.030.
26. Xiong Z, Yi L, Cao D, He W, Chen J, Gao S, Sun X (2016). Dravet syndrome with autism inherited from a paternal mosaic heterozygous mutation on SCN1A. J Neurol Sci. 2016 Oct 15;369:53-6. doi: 10.1016/j.jns.2016.07.038.
27. Li, Y., T. Liu, N. Van Halm-Lutterodt,J. Chen, Q. Su and Y. Hai (2016). Reprogramming of blood cells into induced pluripotent stem cells as a new cell source for cartilage repair. Stem Cell Res Ther7(1): 31.
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