姓名：郭占云
学位：博士
导师情况：博士生导师
研究领域：生物化学
研究方向：蛋白质与多肽

E-mail：zhan-yun.guo@tongji.edu.cn或guozy2002@yahoo.com.cn
联系电话：021-6598-8634;135-8574-5920
通讯地址：上海市四平路1239号，同济大学生命科学与技术学院蛋白质研究所，医学楼辅楼303房间

个人简介：
郭占云，博士，研究员，博士生导师。1972年生，河北滦南人。1996年毕业于吉林大学分子生物学系，获学士学位。因成绩优异，同年免试进入中国科学院上海生物化学研究所攻读博士学位，在冯佑民研究员实验室从事胰岛素超家族多肽研究。2001年8月获生物化学与分子生物学博士学位。2001-2003年在生物化学与细胞生物学研究所工作，历任助理研究员和副研究员。2003年9月到美国DartmouthCollege医学院生物化学系从事博士后研究，主要研究胆固醇酯合酶1（ACAT1）结构与功能。2006年底回国到同济大学蛋白质研究所工作。已在JBiolChem,Biochemistry,ProteinScience等国内外知名科技期刊上以第一作者（含共同第一作者）或通讯作者（含共同通讯作者）发表SCI论文近30篇。目前作为项目（课题）负责人正主持国家自然科学基金面上项目2项；国家“重大新药创制”科技重大专项（候选药物）1项；国家重点基础研究发展计划（973）课题一项。
主要从事以下研究：
1.胰岛素家族多肽结构与功能研究；
2.多肽靶向药物研究；
3.内源性硫化氢作用机理研究。

发表论文:1.LuoX,BathgateRA,ZhangWJ,LiuYL,ShaoXX,WadeJD*,GuoZY*.(2010)Designandrecombinantexpressionofinsulin-likepeptide5(INSL5)precursorsandthepreparationofmaturehumanINSL5.AminoAcids,inminorrevision.(*correspondingauthors)
2.WuXC,ZhouM,PengC,ShaoXX,GuoZY*,ChiCW*(2010)Novelconopeptidesinaformofdisulfide-crosslinkeddimer.Peptides,accepted.(*correspondingauthors)
3.WangZQ,ZhouZM,GuoZY*,ChiCW*(2010)SnapshotoftheinteractionbetweenHIVenvelopeglycoprotein120andproteindisulfideisomerase.ActaBiochimBiophysSin,accepted(*correspondingauthors)
4.ZhangWJ,LuoX,GuoZY*(2010)InVitroDegradationofInsulin-likePeptide3byInsulin-DegradingEnzyme.ProteinJ,publishedonline(*correspondingauthor)
5.LuoX,BathgateRA,LiuYL,ShaoXX,WadeJD*,GuoZY*.(2009)Recombinantexpressionofaninsulin-likepeptide3(INSL3)precursoranditsenzymaticconversiontomaturehumanINSL3.FEBSJ,276:5203-5211.(*correspondingauthors)
6.YuanDD,LiuL,ShaoXX,PengC,ChiCW*,GuoZY*(2009)NewconotoxinsdefinethenovelI3-superfamily.Peptides,30:861-865.(*correspondingauthors)
7.LiuYL,JiangS,KeZM,WuHS,ChiCW,GuoZY*(2009)Recombinantexpressionofachitosanaseanditsapplicationinchitosanoligosaccharideproduction.CarbohydRes,344:815-819.(*correspondingauthor)
8.YuanDD,LiuL,ShaoXX,PengC,ChiCW*,GuoZY*(2008)IsolationandcloningofaconotoxinwithanovelcysteinepatternfromConuscaracteristicus.Peptides,29:1521-1525.(*correspondingauthors)
9.QianJ,GuoZY*,ChiCW*.(2008)Cloningandisolationofaconuscysteine-richproteinhomologoustoTex31butwithoutproteolyticactivity.ActaBiochimBiophysSin,40:174-181.(*correspondingauthors)
10.ZhangZJ,WuL,QiaoZS,QiaoMQ,FengYM*,GuoZY*(2008)ContributionoftheconservedA16Leutoinsulinfoldability.ProteinJ,27:192-196.(*correspondingauthors)
11.GuoZY*,QiaoZS*,FengYM(2008)Theinvitrooxidativefoldingoftheinsulinsuperfamily.AntioxidRedoxSignal,10:127-139.(*co-firstauthors)
12.GuoZY,ChangCCY,ChangTY(2007)Functionalityoftheseventhandeighthtransmembranedomainsofacyl-coenzymeA:cholesterolacyltransferase1.Biochemistry,46:10063-10071.
13.WangZQ,HanYH,ShaoXX,ChiCW*,GuoZY*(2007)Molecularcloning,expression,andcharacterizationofproteindisulfideisomerasefromConusmarmoreus.FEBSJ,274:4778-4787.(*correspondingauthors)
14.GuoZY,LinS,HenienJA,ChangCY,ChangTY(2005)TheactivesiteHis-460ofhumanacyl-coenzymeA:cholesterolacyltransferase1residesinahithertoundisclosedtransmembranedomain.JBiolChem,280:37814-37826.
15.GuoZY,ChangCY,LuX,ChenJ,LiBL,ChangTY(2005)Thedisulfidelinkageandthefreesulfhydrylaccessibilityofacyl-coenzymeA:cholesterolacyltransferase1asstudiedbyusingmPEG5000-maleimide.Biochemistry,44:6537-6546.
16.GuoZY,ZhangZ,JiaXY,TangYH,FengYM(2005)MutationalanalysisoftheabsolutelyconservedB8Gly:consequenceonfoldabilityandactivityofinsulin.ActaBiochimBiophysSin,37:673-679.
17.GuoZY,JiaXY,FengYM(2004)Replacementoftheinterchaindisulfide-formingaminoacidsA7andB7byglutamateimpairsthestructureandactivityofinsulin.BiolChem,385:1171-1175.
18.GuoZY,WangS,TangYH,FengYM(2004)Mutagenesisofthethreeconservedvalineresidues:consequenceonthefoldabilityofinsulin.BiochimBiophysActa,1699:103-109.
19.JiaXY*,GuoZY*,WangY,XuY,DuanSS,FengYM(2003)Peptidemodelsoffourpossibleinsulinfoldingintermediateswithtwodisulfides.ProteinSci,12:2412-2419.(*co-firstauthors)
20.WangS*,GuoZY*,ShenL,FengYM(2003)Refoldingoftheamphioxusinsulin-likepeptide:implicationsofabifurcatingevolutionofthedifferentfoldingbehaviorsofinsulinandinsulin-like
growthfactor1.Biochemistry,42:9687-9693.(*co-firstauthors)
21.GuoZY,TangYH,WangS,FengYM(2003)ContributionoftheabsolutelyconservedB8Glytothefoldabilityofinsulin.BiolChem,384:805-809.
22.YanH*,GuoZY*,GongYW,XiD,FengYM(2003)Apeptidemodelofinsulinfoldingintermediatewithonedisulfide.ProteinSci,12:768-775.(*co-firstauthors)
23.GuoZY,ShenL,GuW,WuAZ,MaJG.,FengYM(2002)Invitroevolutionofamphioxusinsulin-likepeptidetomammalianinsulin.Biochemistry,41:10603-10607.
24.GuoZY,ShenL,FengYM(2002)ThedifferentenergeticstateoftheintraA-chain/domaindisulfideofinsulinandinsulin-likegrowthfactor1ismainlycontrolledbytheirB-chain/domain.Biochemistry,41:10585-10592.
25.GuoZY,ShenL,FengYM(2002)Thedifferentfoldingbehaviorofinsulinandinsulin-likegrowthfactor1ismainlycontrolledbytheirB-chain/domain.Biochemistry,41:1556-1567.
26.GuoZY,FengYM(2002)ThethermodynamicstabilityofinsulindisulfidesisnotaffectedbytheC-domainofinsulin-likegrowthfactor1.SciChina(SeriesC),45:245-250.
27.GuoZY,FengYM(2001)Effectsofcysteinetoserinesubstitutionsinthetwointer-chaindisulfidebondsofinsulin.BiolChem,382:443-448
28.GuoZY,TangYH,ZhangZ,FengYM(2001)Mutationalanalysisofthethreeconservedvalineresiduesofinsulinandaproposalof“isostericresidue”.IUBMBLife,52:309-314.
29.GuoZY,QiaoZS,FengYM(2001)Unfoldingofrecombinantsingle-chaininsulinindenaturantscontainingthiolreagents.ActaBiochimBiophysSin,33:65-70.
30.ShenL*,GuoZY*,ChenY,LiuLY,FengYM(2001)Expression,purification,characterizationofampioxusinsulin-likepeptideandpreparationofpolyclonalantibodytoit.
ActaBiochimBiophysSin,33:629-633.(*co-firstauthors)
注：发表论文截止2009年3月5日