摘要/Abstract
摘要： 目的 ·分析宁夏枸杞（ Lycium barbarum L.）和黑果枸杞（ Lycium ruthenicum Murr.）二者果实中多酚类物质的组成及差异。方法 ·使用超高效液相色谱 -电喷雾四级杆串联飞行时间质谱（ ultra-high performance liquid chromatography-electrospray ionizationquadrupole-time of flight mass spectrometry，UHPLC-ESI-Q-TOF-MS）对黑果枸杞和宁夏枸杞的鲜果与干果中所含多酚类物质进行系统的定性定量。结果 · 2种枸杞的鲜果和干果中检测并定量酚酸、酚酸酰胺（酚酸与多胺形成的复合物）及花青素共 3类 35种多酚类物质。2种枸杞的干果中多酚类物质的种类多于鲜果，且含量存在物种间差异；黑果枸杞的干果和鲜果中多酚类物质的种类多于宁夏枸杞的干果和鲜果，且 2种枸杞的干果与鲜果中上述物质的含量存在差异。结论 ·黑果枸杞和宁夏枸杞中多酚类物质的种类和含量具有物种依赖性。上述结果为这 2种枸杞果实的药用及食用生理活性的深入研究提供了基础数据。
关键词: 黑果枸杞, 宁夏枸杞, 超高效液相色谱-质谱联用技术, 多酚类物质
Abstract:
Objective · Both Lycium barbarum L. (LB) and Lycium ruthenicum Murr. (LR) belong to the Lycium genus of Solanaceae family but their fruits have significant phenotypic differences in terms of color and shapes. This study is aimed to investigate the inter-species difference of their fruit polyphenol composition. Methods · The polyphenol composition of fresh and dried fruits of two Lycium species were comprehensively analyzed using ultra-high performance liquid chromatography-electrospray ionization-quadrupole-time of flight mass spectrometry (UHPLC-ESIQ-TOF-MS). Results · 35 polyphenolic compounds were detected and quantified in the fresh and dried Lycium fruits including phenolic acids, anthocyanins and phenolamides (i.e., the amide-adducts of polyphenolic acids and polyamines). For both species, the dried fruits had more polyphenolic compounds than their fresh fruits with significant inter-species difference; for both fresh and dried fruits, LR had more polyphenolic compounds than LB. Conclusion · There are significant inter-species differences between these two Lycium in both fresh and dried fruits. These results offer essential information for further understanding of the biological functions of these two Lycium fruits as phytomedicines and functional food.
Key words: Lycium ruthenicum Murr., Lycium barbarum L., ultra-high performance liquid chromatography-electrospray ionization-quadrupole-time of flight mass spectrometry(UHPLC-ESI-Q-TOF-MS), polyphenolic compounds
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