摘要/Abstract

摘要： 目的 ·寻找具有感染耐碳青霉烯类鲍曼不动杆菌 (carbapenem-resistant Acinetobacter baumannii, CRAB)潜力的烈性噬菌体，进行生物学特性及基因组学分析。方法 ·采集污水，富集分离纯化得到噬菌体，通过电子显微镜观察噬菌体形态，研究其理化特征。抽取噬菌体 DNA，进行测序和基因分析。结果 ·分离得到 l 株鲍曼不动杆菌噬菌体，命名为 SH-Ab 15599。电子显微镜观察结果显示该噬菌体为肌尾噬菌体。 SH-Ab 15599能够裂解 51株 CRAB临床分离株中的 15株 (29.4%)；在 4～ 50℃和 pH值 6～ 9的环境下， 1 h内能保持活性；与宿主菌共培养 5 min后，吸附率约为 90%；其潜伏期约 10 min，裂解期约 20 min，每个细胞裂解量约 43 pfu。在体外实验中，宿主菌的生长受到抑制。 SH-Ab 15599的基因组为双链线状 DNA，含 143 204 bp，GC含量为 38.45%。结论 · SH-Ab 15599是一株全新的噬菌体，具有治疗 CRAB感染的潜力。
关键词: 鲍曼不动杆菌, 耐碳青霉烯类鲍曼不动杆菌, 噬菌体, 生物学特性, 基因组学
Abstract:
Objective · To find lytic bacteriophages which have potential to combat carbapenem-resistant Acinetobacter baumannii (CRAB) infections and analyze the biological characterizations and genomic sequences of the phages. Methods · The phages were isolated sewage followedamplification and purification. They were characterizedthe transmission electron microscopy, the host specificity, the biological characterizations, the lysis assay in vitro and the genome sequence analysis. Results · A novel bacteriophage, SH-Ab 15599, was isolated sewage. The electron microscope revealed SH-Ab 15599 belongs to Myoviridae. Among the 51 clinical CRAB isolates tested, 15 (29.4%) were lysedthe phage. SH-Ab 15599 was relatively sat temperature range 4 ℃ to 50 ℃ or between pH 6 and 9. About 90% of the phage was approximately adsorbed to the host CRAB isolate in 5 min. The one-step growth curve showed that the latent period was about 10 min and the growth period was about 20 min. The burst size was estimated at 43 pfu per infected cell. The growth of the host CRAB isolate was inhibitedthe phage in vitro. The genome of SH-Ab 15599 was a linear, 143 204 bp double-stranded DNA with GC content of 38.45%. Conclusion · SH-Ab 15599 is a novel phage targeting CRAB and has potential as an alternative against CRAB infections in the future.
Key words: Acinetobacter baumannii, carbapenem-resistant Acinetobacter baumannii, bacteriophage, biological characteristics, genomics


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