摘要/Abstract

摘要： 目的 ·观察白藜芦醇（ resveratrol，RSV）对小鼠结肠癌细胞 CT26增殖以及凋亡的影响，并探讨其中相关的分子机制。方法 ·梯度浓度 20、40、60、80、100 μmol/L的 RSV孵育细胞后， CCK-8试剂盒检测 RSV对 CT26细胞增殖的影响； Hoechst 33342活细胞染色液检测 RSV对细胞凋亡的影响； Western blotting技术对细胞中凋亡相关的蛋白（ Bax、Bcl-2），核转录因子（ NF-κB）以及 Akt、PTEN的表达量进行检测。结果 · RSV能够抑制 CT26细胞的增殖并促进其凋亡，抑制作用具有浓度依赖性。 RSV能使细胞核内的 NF-κB蛋白的表达量下调，细胞质内的 NF-κB上调； Bax及 PTEN蛋白表达量上调， Bcl-2下调， Akt蛋白的磷酸化下调。结论 · RSV能够抑制 CT26细胞的增殖，其作用可能与上调 Bax和 PTEN蛋白，下调 Bcl-2、Akt磷酸化及 NF-κB活性有关。
关键词: 白藜芦醇, 结肠癌, 细胞增殖, 凋亡, 小鼠
Abstract:
Objective · To investigate the effect of resveratrol (RSV) on proliferation of mocolonic cancer cells CT26 and its underlying molecular mechanisms. Methods · CT26 cells were incubated with 20, 40, 60, 80 and 100 μmol/L RSV respectively for 24 h. The effect of RSV on cell proliferation was tested with CCK8 kits. The effect of RSV on cell apoptosis was detectedliving cell dye Hoechst 33342. The plasma protein and nucleoprotein of the cells were extracted separatelyusing the nuclear protein and cell plasma protein extraction kits. And the s of apoptosis related proteins (Bax and Bcl-2), PTEN, NF-κB and Akt were detectedWestern blotting. Results · RSV inhibited the proliferation of CT26 cells. The inhibitory effect was dose-dependent. Treatment with RSV promoted the apoptosis of CT26 cells. The of NF-κB protein in the cell nucleus was down-regulated compared to the control cells, and the of NF-κB in the cytoplasm was up-regulated in the CT26 cells after 24 h incubation with RSV. In the CT26 cells after 24 h incubation of RSV, the s of Bax and PTEN were up-regulated, the of Bcl-2 was down-regulated, and the phosphorylation of Akt protein was down-regulated. Conclusion · RSV can inhibit the proliferation of colon cancer cells in mice, which may be associated with Bax, Bcl-2, NF-κB, p-Akt and PTEN.
Key words: resveratrol, colonic cancer, proliferation, apoptosis, mouse


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