摘要/Abstract

通过过表达正调控基因的方法成功激活金色链霉菌SP-371中的沉默基因簇tjh，突变株可以产生两种骨架结构的化合物.据推测两种结构的化合物应该是从同一中间体经过两种不同的后修饰途径得到的.通过双基因敲除的方法截断了两种化合物的后修饰途径，成功积累了化合物Aureuspiro.Aureuspiro是一个新的天然产物且结构新颖，含有一个特殊的螺-萘醌结构.该化合物虽然不是最终化合物生物合成途径中的真实中间体，但也进一步辅证了共同中间体结构的推测.
关键词: 基因组挖掘, 生物合成, II型聚酮类天然产物, 螺环
By overexpressing the positive regulatory gene, the silent gene cluster tjh in Streptomyces aureus SP-371 was successfully activated. The mutant strain could produce compounds with two distinct core strucutures which are pentacyclic and tetracyclic structures. It was speculated that these two types of skeletons might be derived from the same intermediate through two different post-modified pathways. These two tailoring pathways were intercepted by the double gene knockout, and aureuspiro was successfully accumulated in the mutant. Aureuspiro is a new natural product with a novel spiro-naphthoquinone structure. Despite aureuspiro is not a true intermediate in the biosynthesis of final products, it could support the hypothesis about structure of the common intermediate.
Key words: genome mining, biosynthesis, type II polyketides natural product, spiro ring


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