摘要/Abstract
利用硅胶、高效液相等多种色谱技术对大果榕根的化学成分进行系统研究，从中分离得到5个异黄酮类化合物，根据理化性质、NMR、IR、UV-Vis等多种现代波谱技术鉴定上述化合物分别为5，7，4'-三羟基-8-（1-羟基-2，3-环氧-3-甲基丁基）异黄酮（1），5，7，4'-三羟基-3'-（2，6，7-三羟基-3-亚甲基-7-甲基辛基）异黄酮（2），5，7，4'-三羟基-6-[（S）-2，3-二羟基-3-甲基丁基]异黄酮（3），5，7，4'-三羟基-6-异戊烯基异黄酮（4），5，7，4'-三羟基-3'-异戊烯基-异黄酮（5）.其中化合物1和2为新化合物，对化合物1~5进行体外抗骨质疏松活性测试，结果表明化合物4和5在100 μmol/L时具有一定的抗骨质疏松活性.
关键词: 大果榕, 化学成分, 异黄酮, 抗骨质疏松活性
Five isoflavonoids were isolated from the roots of the Ficus auriculata by means of silica gel column, HPLC and other chromatography methods. By using spectroscopic techniques including NMR, IR and UV, their structures were identified as 5,7,4'-trihydroxy-8-(1-hydroxy-2,3-epoxy-3-methylbutyl)isoflavone (1), 5,7,4'-trihydroxy-3'-(2,6,7-trihydroxy-3-methylene-7-methyloctyl)isoflavone (2), 5,7,4'-trihydroxy-6-[(S)-2,3-dihydroxy-3-methylbutyl]isoflavone (3), 5,7,4'-trihydroxy-6-pre-nylisoflavone (4), and 5,7,4'-trihydroxy-3'-prenylisoflavone (5). Compounds 1 and 2 were new isoflavonoids. All compounds were evaluated for their anti osteoporosis activities in vitro. Compounds 4 and 5 showed moderate proliferation function of primary osteoblasts in 100 μmol/L.
Key words: Ficus auriculata, chemical constituent, isoflavone, anti osteoporosis activity
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