番茄红素对胰腺alpha和beta细胞的差异作用
柳卫凰, 王巧娜, 周颖, 王妍君, 童攒^*
武汉大学基础医学院，武汉 430071
摘要
番茄红素是一种强抗氧化剂，在糖尿病的治疗实验中显示出对机体的保护作用，但是其细胞机制尚不明确。本研究 采用不同浓度梯度的番茄红素处理胰腺alpha和beta细胞系，检测细胞的生长、凋亡、周期、活性氧、ATP水平和相关细胞因 子的表达变化。结果显示，番茄红素不影响alpha细胞的生长、凋亡、周期、活性氧和ATP水平，但番茄红素可以促进beta细 胞的生长、上调其S期比例、降低活性氧水平并提升ATP水平。与此同时，番茄红素可以提升beta细胞tnfα、tgfβ和hif1α mRNA的表达。这些结果表明番茄红素的作用具有细胞特异性，可以活化胰腺beta细胞，为其在糖尿病防治的临床应用提供 数据支撑。


关键词： 番茄红素; 胰腺alpha细胞; 胰腺beta细胞; 细胞周期; 活性氧
分类号：Q25

Divergent effects of lycopene on pancreatic alpha and beta cells
LIU Wei-Huang, WANG Qiao-Na, ZHOU Ying, WANG Yan-Jun, TONG Zan^*
School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China
Abstract
Lycopene is an antioxidant which has potential anti-diabetic activity, but the cellular mechanisms have not been clarified. In this study, different concentrations of lycopene were used to treat pancreatic alpha and beta cell lines, and the changes of cell growth, cell apoptosis, cell cycle, reactive oxygen species (ROS), ATP levels and expression of related cytokines were determined. The results exhibited that lycopene did not affect cell growth, cell apoptosis, cell cycle, ROS and ATP levels of alpha cells, while it promoted the growth of beta cells, increased the ratio of S phase, reduced the ROS levels and increased the ATP levels of beta cells. At the same time, lycopene treatment elevated the mRNA expression levels of tnfα, tgfβ and hif1α in beta cells. These findings suggest that lycopene plays cell-specific role and activates pancreatic beta cells, supporting its application in diabetes therapy.



Key words: lycopene; pancreatic alpha cell; pancreatic beta cell; cell cycle; reactive oxygen species

收稿日期：2019-08-21　　录用日期：2020-02-27
通讯作者：童攒　　E-mail: ztong@whu.edu.cn
DOI: 10.13294/j.aps.2020.0017
引用本文：
柳卫凰, 王巧娜, 周颖, 王妍君, 童攒. 番茄红素对胰腺alpha和beta细胞的差异作用[J]. 生理学报 2020; 72 (2): 133-138.
LIU Wei-Huang, WANG Qiao-Na, ZHOU Ying, WANG Yan-Jun, TONG Zan. Divergent effects of lycopene on pancreatic alpha and beta cells. Acta Physiol Sin 2020; 72 (2): 133-138



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