王金研究员

教育和职业经历:
09/2000-07/2004南京农业大学生物科学专业理学学士学位
09/2004-07/2009中科院上海生命科学研究院植物生理生态研究所微生物学博士学位
07/2009-07/2012香港中文大学微生物学系博士后
07/2012-11/2013中科院上海生命科学研究院植物生理生态研究所助理研究员
12/2013-04/2018中科院上海生命科学研究院植物生理生态研究所副研究员
05/2018-上海师范大学研究员（独立研究组）
个人研究方向：
主要研究方向是合成生物学使能技术的开发及其应用。近年来，申请人以第一作者或通讯作者身份在Cell Research、Nucleic Acids Research、ACS Synthetic Biology、Cell Discovery等杂志发表论文20多篇，并申请了多项发明专利。其中主要的成果包括：（1）开发了新型DNA体外无缝拼接/编辑技术，提高了大片段、复杂片段DNA的体外拼接/编辑效率和准确率。（2）提出了基于CRISPR/Cas12a的新型生物元器件拼接标准（C-Brick）。（3）开发了基于CRISPR的微生物基因编辑/基因调控技术，建成了不依赖于PAM位点的基因组编辑方法和基于CRISPR crRNA阵列的多基因高效转录调控的新方法。（4）开发了CRISPR新型分子诊断系统，该系统被Science杂志誉为下一代分子诊断技术。

发表论文(# first author; * corresponding author):
Jin Wangand Guoping Zhao*. (2009) GlnR positively regulates nasA transcription in Streptomyces coelicolor.Biochemical and Biophysical Research Communications386 (1): 77-81.
Wei Zhao^#, Yi Zhong^#, Hua Yuan^#, Jin Wang^#, et al.(2010) Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterraneiU32 revealed its genetic characteristics in phylogeny and metabolism. Cell Research20(10):1096-1108.
Ying Wang, XuFeng Cen, GuoPing Zhao* and Jin Wang* (2012) Characterization of a New GlnR Binding Box in the Promoter of amtBin Streptomyces coelicolorInferred a PhoP/GlnR Competitive Binding Mechanism for Transcriptional Regulation of amtB. Journal of Bacteriology194(19): 5237-5244.
WeiHua Chen, ZhongJun Qin, Jin Wang* and GuoPing Zhao* (2013) The MASTER (Methylation-Assisted Tailorable Ends Rational) Ligation method for seamless DNA assembly.Nucleic Acids Research41(8): e93.
Ying Wang, JingZhi Wang, ZhiHui Shao, Hua Yuan, YinHua Lu, WeiHong Jiang, GuoPing Zhao*, Jin Wang*(2013) Three of Four GlnR Binding Sites Are Essential for GlnR-Mediated Activation of Transcription of theAmycolatopsis mediterranei nasOperon. Journal of Bacteriology195(11): 2595-2602.
Ying Wang^#, Chen Li^#, Na Duan^#, Bing Li, XiaoMing Ding, YuFeng Yao, Jun Hu*, GuoPing Zhao*, Jin Wang*. (2014) GlnR Negatively Regulates the Transcription of the Alanine Dehydrogenase Encoding Gene ald in Amycolatopsis mediterraneiU32 under Nitrogen Limited Conditions via Specific Binding to Its Major Transcription Initiation Site. PLoS One9(8): e104811.
JiaKun Liu, WeiHua Chen, ShuangXi Ren, GuoPing Zhao*,Jin Wang*. (2014) iBrick: a new standard for iterative assembly of biological parts with homing endonucleases. PLoS One9(10): e110852.
Jin Wang*, Ying Wang, GuoPing Zhao (2015) Precise characterization of GlnR Box in actinomycetes. Biochemical and Biophysical Research Communications458(3): 605-607.
ZhuiHui Shao, ShuangXi Ren, XinQiang Liu, Jian Xu, Han Yan, GuoPing Zhao, Jin Wang*(2015) A preliminary study of the mechanism of nitrate-stimulated remarkable increase of rifamycin production in Amycolatopsis mediterraneiU32 by RNA-seq. Microbial Cell Factories 14: 75.
ZhiHui Shao^#, WanXin Deng^#, ShiYuan Li, JuanMei He, ShuangXi Ren, WeiRen Huang, YinHua Lu, GuoPing Zhao, ZhiMing Cai*, Jin Wang*(2015) GlnR-Mediated Regulation of ectABCDTranscription Expands the Role of the GlnR Regulon to Osmotic Stress Management. Journal of Bacteriology197(19): 3041–3047.
XuFeng Cen, JingZhi Wang, GuoPing Zhao, Ying Wang*, Jin Wang*(2016) Molecular evidence for the coordination of nitrogen and carbon metabolisms, revealed by a study on the transcriptional regulation of the agl3EFG operon that encodes a putative carbohydrate transporter in Streptomyces coelicolor. Biochemical and Biophysical Research Communications471(4):510-514.
ShiYuan Li, GuoPing Zhao*, Jin Wang*(2016) C-Brick: A New Standard for Assembly of Biological Parts Using Cpf1.ACS Synthetic Biology5(12):1383-1388.
Chao Lei^#, ShiYuan Li^#, JiaKun Liu^#, Xuan Zheng, GuoPing Zhao, Jin Wang*(2017) The CCTL (Cpf1-assisted Cutting and Taq DNA ligase-assisted Ligation) method for efficient editing of large DNA constructs in vitro. Nucleic Acids Research45(9): e74.
Chen Li^#, XinQiang Liu^#, Chao Lei, Han Yan, ZhiHui Shao, Ying Wang, GuoPing Zhao, Jin Wang*, XiaoMing Ding* (2017) RifZ (AMED_0655) is a pathway-specific regulator for rifamycin biosynthesis in Amycolatopsis mediterranei.Applied Environmental Microbiology83(8): e03201-16
Xuan Zheng^#, ShiYuan Li^#, GuoPing Zhao, Jin Wang*(2017) An efficient system for deletion of large DNA fragments in Escherichia coliviaintroduction of both Cas9 and the non-homologous end joining system from Mycobacterium smegmatis. Biochemical and Biophysical Research Communications485(4): 768-774
ShiYuan Li, GuoPing Zhao, Jin Wang*(2017) Protocols for C-Brick DNA standard assembly using Cpf1.Journal of Visualized Experiments(124), e55775
Hong Zhang, Qiuxiang Cheng, Aimin Liu*, Guoping Zhao, Jin Wang*(2017) A novel and efficient method for bacteria genome editing employing both CRISPR/Cas9 and an antibiotic resistance cassette. Frontiers in Microbiology8: 812
Xiaochun Zhang^#, Jingman Wang^#, Qiuxiang Cheng^#, Xuan Zheng, Guoping Zhao, Jin Wang* (2017) Multiplex gene regulation by CRISPR–ddCpf1. Cell Discovery3: 17018
Chao Lei^#, Jingzhi Wang^#, Yuanyuan Liu, Xinqiang Liu, Guoping Zhao, Jin Wang*(2018) A feedback regulatory model for RifQ-mediated repression of rifamycin export in Amycolatopsismediterranei. Microbial Cell Factories17:14
Shi-Yuan Li, Qiu-Xiang Cheng*, Jia-Kun Liu, Xiao-Qun Nie, Guo-Ping Zhao and Jin Wang* (2018) CRISPR-Cas12a has both cis-and trans-cleavage activities on single-stranded DNA. Cell Research28: 491–493.
Zhendong Li^#, Xinqiang Liu^#, Jingzhi Wang, Ying Wang, Guosong Zheng, Yinhua Lu, Guoping Zhao, Jin Wang* (2018) Insight into the molecular mechanism of the transcriptional regulation of amtBoperon in Streptomyces coelicolor. Frontiers in Microbiology9: 264
Shi-Yuan Li^#, Qiu-Xiang Cheng^#, Jing-Man Wang, Xiao-Yan Li, Zi-Long Zhang, Song Gao, Rui-Bing Cao, Guo-Ping Zhao and Jin Wang*(2018) CRISPR-Cas12a-assisted nucleic acid detection. Cell Discovery4: 20.
Shi-Yuan Li, Jia-Kun Liu, Guo-Ping Zhao and Jin Wang*(2018) CADS: CRISPR/Cas12a-Assisted DNA Steganography for Securing the Storage and Transfer of DNA-encoded Information. ACS Synthetic Biology7(4):1174-1178.
Yi Li^#, Shi-Yuan Li^#, Jin Wang*, Guo-Zhen Liu* (2018) CRISPR/Cas Systems towards Next-Generation Biosensing. Trends in Biotechnology37(7): 730-743; DOI:https://doi.org/10.1016/j.tibtech.2018.12.005
Jingman Wang^#, Anrui Lu^#, Jinxin Bei*, Weiren Huang, Zhiming Cai, Guoping Zhao*, Jin Wang* (2019) CRISPR/ddCas12a-based programmable and accurate gene regulation. Cell Discovery 5: 15.
Anrui Lu^#, Jingman Wang^#, Weihong Sun*, Weiren Huang*, Zhiming Cai*, Guoping Zhao, Jin Wang*(2019) Reprogrammable CRISPR/dCas9-based recruitment of DNMT1 for site-specific DNA demethylation and gene regulation. Cell Discovery 5: 22.

E-mail:wangj01@hotmail.com