姓名： 厉萍 导师类别： 硕导

所在专业： 水产养殖 研究方向：
学院/单位： 水科院长江所
专业所属学院： 水产与生命学院
专业技术职务： 副研究员 行政职务：
性别： 女 E-Mail： liping@yfi.ac.cn
最高学位： 博士
通讯地址：
备注：
简历

厉萍，中共党员，博士。2004年7月毕业于山东师范大学生命科学学院，获学士学位；2007年7月获华中农业大学水产学院湿地资源与环境专业硕士学位；同年10月赴捷克南波希米亚大学水产养殖与水资源保护学院从事鱼类精液超低温冷冻保存技术及精子冷冻损伤机制研究，于2011年10月获得博士学位；并于2011年11月赴新加坡国立大学生物系从事博士后研究工作，主要运用代谢组学和iTRAQ标记的蛋白质组学研究水体污染物对鱼类影响的分子机制；2012年12月回国至今，主要从事濒危鱼类保护研究工作。
到目前为止，发表SCI论文43篇，其中第一作者（或通讯作者）13篇，文章被引用次数240次，H-index 14。此外，本人还担任PloS ONE; BioMed Research International; Reproduction, Fertility and Development; Fish Physiology and Biochemistry 等SCI期刊的独立审稿人。留学期间，曾主持并完成了由捷克南波希米亚大学资助的两项课题。2010年获得由国家留学基金委颁发的国家优秀自费留学生奖学金。
研究领域
围绕濒危鱼类保护研究工作，主要从事三个方面的研究。第一，鱼类精液超低温冷冻保存技术研究，建立鱼类精子保存库，保护鱼类种质资源和生物遗传多样性，可将优良和珍稀濒危鱼类种质长期保存下来，可以解决雌雄鱼成熟不同步、地理隔离导致某些鱼类之间难以交配的难题，还可以为鱼类雌核发育等研究不间断地提供精子材料，大大方便遗传育种的研究工作。第二，采用蛋白质组学技术寻找与精子质量相关的分子标志物及其生理功能的调控机制，有助于深入系统地研究鱼类精子蛋白质组成、代谢和功能，探讨影响冻精活率和受精能力的根本原因，稳定有效地提高濒危鱼类精液冷冻效果，进一步提高受精率和孵化率，为濒危鱼类种质资源保护提供有价值的参考依据。第三，鱼类生殖细胞分离、培养、增殖和保存技术研究，该技术与细胞移植技术结合，为鱼类种质细胞的保存开辟了新的领域。
近期主持的课题
国家自然基金青年项目，鲟鱼精子质量蛋白质标志物的筛选与功能研究（**），2015/01-2017/12，24万元，在研，主持。
中央级公益性科研院所基本科研业务费，水产生物生殖调控相关机制及技术研究-达氏鲟精原干细胞体外增殖与冷冻保存（2015B02YQ01），2015/01- 2017/12，28万元，在研，主持。
公益性行业（农业）科研专项，珍稀水生生物繁育与物种保护技术研究---部分课题：配子保存技术研究（），2014/01-2016/12，20元，在研，主持。
中国水产科学研究院长江水产研究所人才引进科研启动经费，鲟鱼精子冷冻生物学研究，2013/01-2013/12，10万元，已结题，主持。

教育经历


工作经历


研究成果


获奖情况


专利著作


论文发表

文章列表
Li, P.*, Hulak, M., Li, Z.H., Sulc, M., Psenicka, M., Rodina, M., Gela, D., Linhart., O., 2013. Cryopreservation of common carp (Cyprinus carpio L.) sperm induces protein phosphorylation in tyrosine and threonine residues. Theriogenology, 80(2): 84-89.
Li, P.*, Li, Z.H.*, Hulak, M., Rodina, M., Linhart, O., 2012. Regulation of spermatozoa motility in response to cations in Russian sturgeon (Acipenser gueldenstaedtii). Theriogenology 78(1): 102-109.
Li, P.*, Rodina, M., Hulak, M., Li, Z.H., Linhart, O., 2011. Spermatozoa concentration, seminal plasma composition and their physiological relationship in the endangered stellate sturgeon (Acipenser stellatus) and Russian sturgeon (Acipenser gueldenstaedtii). Reproduction in Domestic Animals 46(2): 247-252.
Li, P.*, Rodina, M., Hulak, M., Gela, D., Psenicka, M., Li, Z.H., Linhart, O., 2011. Physico-chemical properties and protein profiles of sperm from three freshwater chondrostean species: a comparative study among Siberian sturgeon (Acipenser baerii), sterlet (Acipenser ruthenus) and paddlefish (Polyodon spathula). Journal of Applied Ichthyology 27(2): 673-677.
Li, P.*, Li, Z.H., Dzyuba, B., Hulak, M., Rodina, M., Linhart, O., 2010. Evaluating the impacts of osmotic and oxidative stress on common carp (Cyprinus carpio, L.) sperm caused by cryopreservation techniques. Biology of Reproduction 83(5): 852-858.
Li, P.*, Dzyuba, B., Hulak, M., Rodina, M., Boryshpolets, S., Li, Z.H., Linhart, O., 2010. Percoll gradient separation of cryopreserved common carp spermatozoa to obtain a fraction with higher motility, velocity and membrane integrity. Theriogenology, 74(8): 1356-1361.
Li, P.*, Hulak, M., Koubek, P., Sulc, M., Dzyuba, B., Boryshpolets, S., Rodina, M., Gela, D., Manaskova-Postlerova, P., Peknicova, J., Linhart., O., 2010. Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L. Theriogenology 74(3): 413-423.
Li, P.*, Hulak, M., Rodina, M., Sulc, M., Li, Z.H., Linhart, O., 2010. Comparative protein profiles: potential molecular markers from spermatozoa of Acipenseriformes (Chondrostei, Pisces). Comparative Biochemistry and Physiology: Part D: Genomics and Proteomics 5(4): 302-307.
Li, P.*, Rodina, M., Hulak, M., Gela, D., Li, Z.H., Linhart, O., 2010. Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso. Journal of Applied Ichthyology 26(5): 753-755.
Li, P., Hulak, M., Linhart, O., 2009. Sperm proteins in teleostean and chondrostean (sturgeon) fishes. Fish Physiology and Biochemistry 35(4):567-581.
Li, P., Wei, Q., Liu, L., 2008. DNA integrity of Polyodon spathula cryopreserved sperm. Journal of Applied Ichthyology 24(2): 121-125.
Li, Z.H.*, Li, P.*, Sulc, M., Hulak, M., Randak, T., 2012. Hepatic proteome sensitivity in rainbow trout after chronically exposed to a human pharmaceutical Verapamil. Molecular and Cellular Proteomics, 11(1): M111.008409.
Li,Z.H*.,Li,P.2015.EvaluationoftributyltintoxicityinChineserareminnowlarvaebyabnormalbehavior,energymetabolismandendoplasmicreticulumstress.Chemico-BiologicalInteractions,227,32-36.
LiZ.H*.,ChenL.,WuY.H.,LiP.,LiY.F.,&NiZ.H.2014.EffectsofwaterbornecadmiumonthyroidhormonelevelsandrelatedgeneexpressioninChineserareminnowlarvae.ComparativeBiochemistryandPhysiologyPartC:Toxicology&Pharmacology,161,53-57.
LiZ.H*.,ChenL.,WuY.H.,LiP.,LiY.F.,&NiZ.H.2014.EffectsofmercuryonoxidativestressandgeneexpressionofpotentialbiomarkersinlarvaeoftheChineserareminnowGobiocyprisRarus.ArchivesofEnvironmentalContaminationandToxicology,67,245-251.
LiZ.H*.,ChenL.,WuY.H.,LiP.,LiY.F.,&NiZ.H.2014.AlterationofthyroidhormonelevelsandrelatedgeneexpressioninChineserareminnowlarvaeexposedtomercurychloride.EnvironmentalToxicologyandPharmacology,38,325-331.
LiZ.H*.,LiP.,&ShiZ.C.2014.Molecularresponsesindigestivetractofjuvenilecommoncarpafterchronicexposuretosublethaltributyltin.EcotoxicologyandEnvironmentalSafety,109,10-14.
Li,Z.H*.,Li,P.*,Chen,L.,2014.TemperatureaffectsHg-inducedantioxidantresponsesinChineserareminnowGobiocyprisraruslarvaeinvitro.BulletinofEnvironmentalContaminationandToxicology,93,666-669.
Shaliutina, A., Hulak, M., Li, P., Sulc, M., Dzyuba, B., Linhart, O., 2013. Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus). Reprod Domest Anim. 48(1): 156-159.
Li, Z.H., Zlabek, V., Velisek, J., Grabic, R., Machova, J., Kolarova, J., Li, P., Randak, T. 2013. Multiple biomarkers responses in juvenile rainbow trout, Oncorhynchus mykiss, after acute exposure to a fungicide propiconazole. Environmental Toxicology, 28(3): 119-126.
Shaliutina, A., Dzyuba, B., Hulak, M., Boryshpolets, S., Li, P., Linhart, O., 2012. Evaluation of Spermiation Indices with Multiple Sperm Collections in Endangered Sterlet (Acipenser ruthenus). Reproduction in Domestic Animals 47(3):479-484.
Li, Z.H., Velisek, J., Zlabek, V., Grabic, R., Machova, J., Kolarova, J., Li, P., Randak, T., 2011. Chronic toxicity of Verapamil on juvenile rainbow trout (Oncorhynchus mykiss): effects on morphological indices; hematological parameters and antioxidant responses.Journal of Hazardous Materials 185 (2-3): 870-880.
Li, Z.H., Velisek J., Grabic R., Li P., Kolarova J., Randak T. 2011. Use of hematological and plasma biochemical parameters to assess the chronic effects of a fungicide Propiconazole on a freshwater teleost. Chemosphere, 83(4): 572-578.
Li, Z.H., Li, P., Randak, T. 2011. Evaluating the toxicity of environmental concentrations of waterborne chromium (VI) to a model teleost, Oncorhynchus mykiss: a comparative study of in vivo and in vitro. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 153(4): 402-407.
Li, Z.H., Zlabek, V., Velisek, J., Grabic, R., Machova, J., Kolarova, J., Li, P., Randak, T., 2011. Acute toxicity of carbamazepine to juvenile rainbow trout (Oncorhynchus mykiss): effects on antioxidant responses, hematological parameters and hepatic EROD. Ecotoxicology and Environmental Safety 74 (3): 319-327.
Psenicka, M., Kaspar, V., Alavi, S.M.H., Rodina, M., Gela, D., Li, P., Borishpolets, S., Cosson, J., Linhart, O., Ciereszko, A., 2011. Potential role of the acrosome of sturgeon spermatozoa in the fertilization process. Journal of Applied Ichthyology 27(2): 678-682.
Wei, Q.W., Zou,Y.C., Li, P., Li, L.X., 2011. Sturgeon aquaculture in China: progress, strategies and prospects assessed on the basis of nation-wide surveys (2007–2009). Journal of Applied Ichthyology 27(2): 162-168.
Zou, Y.C., Wei Q.W., Zeng, L.B., Li, P., 2011. Production of gynogenetic diploid Polyodon spathula using fertilization with irradiated sperm followed by spontaneous diploidization and distant hybridization caused by heat shock. Journal of Applied Ichthyology 27(2): 505-509.
Li, Z.H., Zlabek, V., Velisek, J., Grabic, R., Machova, J., Kolarova, J., Li, P., Randak, T., 2011. Antioxidant responses and plasma biochemical characteristics in the freshwater rainbow trout, Oncorhynchus mykiss, after acute exposure to the fungicide propiconazole. Czech Journal of Animal Science 56(2): 64-72.
Li, Z.H., Zlabek, V., Grabic, R., Li, P., Randak, T., 2011. Biochemical responses in gill of rainbow trout exposed to propiconazole. Central European Journal of Biology 6 (1): 84-90.
Li Z.H., Li P., Randak T. 2011. Protective roles of calcium channel blocker against cadmium-induced physiological stress in freshwater teleost Oncorhynchus mykiss. Water, Air, & Soil Pollution 220 (1-4): 293-299.
Li, Z.H., Li, P., Rodina, M., Randak, T., 2010. Effect of human pharmaceutical Carbamazepine on the quality parameters and oxidative stress in common carp (Cyprinus carpio L.) spermatozoa. Chemosphere 80(5): 530-534.
Li, Z.H., Zlabek, V., Grabic, R., Li, P., Machova, J., Velisek, J., Randak, T., 2010. Effects of exposure to sublethal propiconazole on the antioxidant defense system and Na+-K+-ATPase activity in brain of rainbow trout, Oncorhynchus mykiss. Aquatic Toxicology 98(3): 297-303.
Li, Z.H., Li, P., Rodina, M., Randak, T., 2010. Evaluating the function of calcium antagonist on the cadmium-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii. Aquatic Toxicology 100(4): 373-375.
Li, Z.H., Li, P., Randak, T., 2010. Ecotoxocological effects of short-term exposure to a human pharmaceutical Verapamil in juvenile rainbow trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 152(3): 385-391.
Li, Z.H., Zlabek, V., Grabic, R., Li, P., Randak, T., 2010. Modulation of glutathione-related antioxidant defense system of fish, chronically treated by the fungicide propiconazole. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 152(3): 392-398.
Li, Z.H., Zlabek, V., Grabic, R., Li, P., Machova, J., Velisek, J., Randak, T., 2010. Effects of exposure to sublethal propiconazole on intestine-related biochemical responses in rainbow trout, Oncorhynchus mykiss. Chemico-Biological Interactions 185(3): 241-246.
Li, Z.H., Li, P., Dzyuba, B., Randak, T. 2010. Influence of environmental related concentrations of heavy metals on motility parameters and antioxidant responses in sturgeon sperm. Chemico-Biological Interactions 188(3):473-477.
Li, Z.H., Zlabek, V., Li, P., Grabic, R., Velisek, J., Machova, J., Randak, T., 2010. Biochemical and physiological responses in liver and muscle of rainbow trout after long-term exposure to propiconazole. Ecotoxicology and Environmental Safety 73(6): 1391-1396.
Hulak, M., Kašpar, V., Psenicka, M., Gela, D. Li, P., Linhart, O., 2010. Does triploidization produce functional sterility of triploid males of tench (Tinca tinca L.)? Reviews in Fish Biology and Fisheries 20(3): 307-315.
Li, Z.H., Li, P., Randak, T., 2010. Effect of a human pharmaceutical carbamazepine on antioxidant responses in brain of a model teleost in vitro: An efficient approach to biomonitoring. Journal of Applied Toxicology 30(7): 644-648.
Boryshpolets, S., Dzyuba, B., Rodina, M., Li, P., Hulak, M., Gela, D., Linhart, O., 2009. Freeze-thawing as the factor of spontaneous activation of spermatozoa motility in common carp (Cyprinus carpio L.). Cryobiology 59(3): 291-296.
Li, Z.H., Xie, S., Wang, J.X., Sales, J., Li, P., Chen, D.Q., 2009. Effect of intermittent starvation on growth and some antioxidant indexes of Macrobrachium nipponense (De Haan). Aquaculture research 40(5): 526-532.
Wei, Q., Li, P., Psenicka, M., Alavi, S.M.H., Shen, L., Liu, J., Peknicova, J., Linhart, O., 2007. Ultrastructure and morphology of sperm in Chinese sturgeon (Acipenser sinensis Gray 1835) using scanning and transmission electron microscopy. Theriogenology 67(7): 1269-1278.