姓名： 李宁求 导师类别： 博导

所在专业： 水产养殖 研究方向： 不区分研究方向
学院/单位： 水科院珠江所
专业所属学院： 水产与生命学院
专业技术职务： 研究员 行政职务： 副主任
性别： 男 E-Mail： liningq@126.com
最高学位： 博士
通讯地址： 广州市荔湾区西朗兴渔路1号
备注：
简历

李宁求，男，湖南长沙人，博士，研究员， 博导，现中国水产科学研究院珠江水产研究所水产病害与免疫研究室副主任，先后入选广州市“珠江科技新星”、中国水产科学研究院“百名科技英才”和中国水产科学研究院“中青年科技拔尖人才”。

教育经历

2011.9-2015.6，上海海洋大学，水产养殖 ，博 士
2001.9-2004.6，上海水产大学，水产养殖，硕士
1997.9-2001.6 ，湖南农业大学 ，水产养殖 ， 学士

工作经历

2019.01-至今，珠江水产研究所 ，研究员
2013.01—2018.12， 珠江水产研究所 ，副研究员
2010.11—至今，珠江水产研究所， 副主任
2007.07—2012.12，珠江水产研究所， 助理研究员
2004.07—2007.07，珠江水产研究所，研究实习员

研究成果

长期从事水产病害防治研究，先后主持国家自然科学基金面上项目、青年基金、国家重点研发计划、广东省海洋渔业科技专项等省部级以上项目十多项，参与国家863、支撑计划（攻关计划）、公益性农业行业专项等重大项目10余项，围绕鳜鲈鳢等名优鱼类主要疾病，组织开展流行病学、病原分离鉴定、疫苗研制与免疫防控技术、生物安保与疫病区域化防控技术研究与应用示范，取得较好的应用效果。成果先后获中国水产科学研究院科技进步奖一等奖2项、三等奖1项、广东省农业技术推广奖二等奖1项；共发表学术论文130余篇，其中SCI收录论文54篇，获授权发明专利10项；参编著作4部。

获奖情况

（1）广谱、高效海水鱼弧菌病重组OmpK亚单位疫苗创制与转基因生物安全评价，中国水产科学研究院科技进步三等奖，第一完成人，2015年；
（2）海水养殖鱼类哈维氏弧菌、溶藻弧菌二联疫苗研究，中国水产科学研究院科技进步奖一等奖，第三完成人，2007年；
（3）淡水鱼类细菌性败血症免疫防控技术关键及产业化应用，中国水产科学研究院科技进步一等奖，第二十完成人，2013年.
（4）鳜鱼免疫与生态防控技术示范与推广，广东省农业技术推广奖二等奖，第二完成人，2019年.

专利著作

（1）一种鱼类烂鳃病病原约氏黄杆菌减毒活疫苗及构建方法。授权号：ZL.9.7，第一发明人；
（2）哈维氏弧菌外膜蛋白OmpK抗原的表达和作为疫苗组份的应用。授权号：ZL.9X，第一发明人；
（3）鳜鱼脑细胞系的构建方法，国家发明专利：ZL 2.9，第二发明人；
（4）一种鳜传染性脾肾坏死病毒荧光定量PCR检测试剂盒及检测方法，国家发明专利：ZL. 5.8，第三发明人；
（5）鳜传染性脾肾坏死病毒（ISKNV）ORF093蛋白的应用，国家发明专利：ZL. 9.0，第三发明人；
（6）一种同时检测水生动物败血症三种致病菌的多重PCR引物组及检测方法， 国家发明专利：ZL.1.5，第四发明人；
（7）一种用于收纳野外分离细菌平板的装置，实用新型专利：ZL. 5.8.，第三发明人；
（8）一种抗传染性脾肾坏死病毒的DNA疫苗, 国家发明专利：1.90, 第一发明人；
（9）一种鳜传染性脾肾坏死病毒灭活快速检验试剂盒及检测方法, 国家发明专利：7.9, 第一发明人；
（10）一种传染性脾肾坏死病毒灭活疫苗有效抗原含量测定方法及试剂盒, 国家发明专利：5.8, 第一发明人；
（11）一种微载体悬浮培养CPB细胞生产鳜传染性脾肾坏死病毒和鳜弹状病毒的方法, 国家发明专利：8.0, 第一发明人.

论文发表

•主要论文：(*为通信作者,以发表时间为序)
英文文章：
Xiaozhe Fu, Yue Ming, Chen Li, Yinjie Niu, Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang, Ningqiu Li*. Siniperca chuatsi rhabdovirus (SCRV) induces autophagy via PI3K/AktmTOR pathway in CPB cells. Fish and Shellfish Immunology 102 (2020) 381–388.
Yinjie Niu, Xiaozhe Fu, Lihui Liu, Qiang Lin, Hongru Liang, Zhibin Huang, Ningqiu Li∗. Molecular characterization and function of EGFR during viral infection process in Mandarin fishSiniperca chuatsi. Fish and Shellfish Immunology 102 (2020) 211–217
Yinjie Niu, Peng Zhang, Luyao Wang, Ningqiu Li, Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang, Xiaozhe Fu. Development of double-antibody sandwich ELISA for rapidly quantitative detection of antigen concentration in inactivated SCRV vaccine. Aquaculture 520 (2020) 734671
Xixi Guo, Yang Zhou, Xiaozhe Fu, Qiang Lin, Lihui Liu, Hongru Liang, Yinjie Niu, Ningqiu Li*. Transcriptomic profiles reveal that inactivated iridovirus and rhabdovirusbivalent vaccine elicits robust adaptive immune responses against lethal challenge in marbled sleepy goby. Fish and Shellfish Immunology 98 (2020) 429–437
Qiang Lin, Jie Li, Xiaozhe Fu, Lihui Liu, Hongru Liang, Yinjie Niu, Chuni Huang,Zhibin Huang, Zhaolan Mo, Ningqiu Li*. Hemorrhagic gill disease of Chinese perch caused byAeromonas salmonicida subsp.salmonicidain China. Aquaculture 519 (2020) 734775
Shangui Liu, Ningqiu Li, Qiang Lin, Lihui Liu, Yinjie Niu, Hongru Liang, Zhibin Huang, Xiaozhe Fu. Glutaminase 1 in mandarin fish Siniperca chuatsi: Molecular characterization, expression pattern and function involving in virus replication. Aquaculture 519 (2020) 734924
Zhao Zhao, Chen Zhang, Qiang Lin, Ning-qiu Li, Zhi-Bin Huang, Mi Zhao, Xiao-Zhe Fu,Gao-Xue Wang, Bin Zhu. Single-walled carbon nanotubes as delivery vehicles enhance the immunoprotective effect of an immersion DNA vaccine against infectious spleen and kidney necrosis virus in mandarinfish. Fish and Shellfish Immunology 97 (2020) 432–439
Xiaozhe Fu, Lixi Zhang, Lihui Liu, Qiang Lin, Hongru Liang, Yinjie Niu, Zhibin Huang, Ningqiu Li*. Identification of intron in ORF003 gene and its application for inactivation test of ISKNV. Microbial Pathogenesis 138 (2020) 103822
Xixi Guo, Shiwei Wu, Ningqiu Li, Qiang Lin, Lihui Liu, Hongru Liang,Yinjie Niu, Zhibin Huang and Xiaozhe Fu. Accelerated metabolite levels of aerobic glycolysis and the pentose phosphate pathway are required for efficient replication of infectious spleen and kidney necrosis virus in Chinese perch brain cells. Biomolecules 2019, 9, 440 Xiaozhe Fu, Xixi Guo, Shiwei Wu, Qiang Lin, Lihui Liu, Hongru Liang, Yinjie Niu and Ningqiu Li*. Non-Targeted UHPLC-Q-TOF/MS-Based Metabolomics Reveals a Metabolic Shift from Glucose to Glutamine in CPB Cells during ISKNV Infection Cycle. Metabolites 2019, 9, 174
Qiang Lin, Yongliang Zhao, Xiaozhe Fu, Lihui Liu, Hongru Liang, Yinjie Niu, Xi ChenZhibin Huang, Li Lin, Ningqiu Li*. Development of strand-specific real-time RT-PCR for the analysis of SCRV transcription and replication dynamics. Microbial Pathogenesis，2019，129 ：146–151
Lihui Liu, Lujun Yu, Xiaozhe Fu, Qiang Lin, Hongru Liang, Yinjie Niu, Ningqiu Li*. First report of megalocytivirus (iridoviridae) in cultured bluegill sunfish,Lepomis macrochirus, in China. Microbial Pathogenesis，2019，135：103617
Zhao Zhao, Chen Zhang, Yi-Jun Jia, De-Kui Qiu, Qiang Lin, Ning-Qiu Li, Zhi-Bin Huang，Xiao-Zhe Fu, Gao-Xue Wang, Bin Zhu. Immersion vaccination of Mandarin fishSiniperca chuatsiagainst infectious spleen and kidney necrosis virus with a SWCNTs-based subunit vaccine. Fish and Shellfish Immunology，2019，92：133–140
Yongliang Zhao, Qiang Lin, Ningqiu Li, V. Sarath Babu, Xiaozhe Fu, Lihui Liu, Hongru Liang, Xiaoling Liu, Li Lin. MicroRNAs profiles of Chinese Perch Brain (CPB) cells infected with Siniperca chuatsirhabdovirus (SCRV). Fish and Shellfish Immunology, 2019:84:1075–1082
Chi Zhang, Ningqiu Li, Xiaozhe Fu, Qiang Lin, Li Lin, Jiagang Tu.MiR-214 inhibits snakehead vesiculovirus (SHVV) replication by targeting host GS. Fish and Shellfish Immunology, 2019:84:299–303
Shiwei Wu, Lujun Yu, Xiaozhe Fu, Xi Yan, Qiang Lin, Lihui Liu, Hongru Liang, Ningqiu Li*. iTRAQ-based proteomic profile analysis of ISKNV-infected CPB cells with emphasizing on glucose metabolism, apoptosis and autophagy pathways。Fish and Shellfish Immunology 2018，79 ：102–111
Zhang Lijuan, Li Ningqiu, Lin Qiang, Liu Lihui, Liang Hongru, Huang Zhibin, Fu Xiaozhe. An avirulent Micropterus salmoides rhabdovirus vaccine candidate protects Chinese perch against rhabdovirus infection. Fish and Shellfish Immunology, 2018, 77: 474-480
Song Yang, Jie Du, Yuan-liang Duan, Qing Xiao, Ning-qiu Li, Qiang Lin, Liu-lan Zhao, Zong-jun Du,Jian Zhou and Jun Du. Differences in the digestive enzyme activity,intestinal mucosa and microbial community in loach cultivated in two separate environments. BMC Microbiology, 2018, 18:113
Chao Ran,Chubin Qin,Mingxu Xie,Jinxiong Zhang,Jie Li,Yadong Xie,Yibing Wang,Shuning Li,Lihui Liu,Xiaozhe Fu,Qiang Li,Ningqiu Li,Mark R. Liles.and Zhigang Zhou. Aeromonas veroniiand aerolysin are important for the pathogenesis of motile aeromonad septicemia in cyprinidfish. Environmental Microbiology , 2018,20(9):3442–3456
Zhendong Qin, V. Sarath Babu, Ningqiu Li, Tairan Fu, Jinquan Li, Lizhu Yi,Lijuan Zhao, Jun Li, Yang Zhou, Li Lin. Protective effects of chicken egg yolk immunoglobulins (IgY) against experimental Aeromonas drophilainfection in blunt snout bream (Megalobrama amblycephala) . Fish and Shellfish Immunology ，2018，78：26–34
Xiaozhe Fu, Xianqin Hu, Ningqiu Li*, Feifei Zheng, Xingxing Dong, Qiang Lin, Jiagang Tu, Lijuan Zhao, Qiwei Qin, Zhibin Huang, Li Lin*. Glutamine and glutaminolysis are required for efficient replication of infectious spleen and kidney necrosis virus in Chinese perch brain cells. Oncotarget, 2017，8（2）：2400-2412
Chen Li, Xiaozhe Fu,Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang, Ningqiu Li*. Autophagy promoted infectious kidney and spleen necrosis virus replication and decreased infectious virus yields in CPB cell line. Fish and Shellfish Immunology, 2017，60：25-32
Min Zhang, Bin Yue, Ai-hua Zhang, Guang-hua Wang, Yong Liu, Shun Zhou, Shun-feng Cheng*, Ning-qiu Li*. TC38, a teleost TFPI-2 peptide that kills bacteria via penetration of the cell membrane and interaction with nucleic acids. Fish & Shellfish Immunology,2017,64:104-110
Shu-wen He, Jian Zhang, Ning-qiu Li, Shun Zhou, Bin Yue, Min Zhang*. A TFPI-1 peptide that induces degradation of bacterial nucleic acids,and inhibits bacterial and viral infection in half-smooth tongue sole, Cynoglossus semilaevis. Fish & Shellfish Immunology, 2017, 60:466-473
（5）Qiang Lin, Xiaozhe Fu, Lihui Liu, Hongru Liang, Huizhi Guo, Shuwen Yin, Venkatesh Kumaresan, Zhibin Huang, Ningqiu Li*. Application and development of a TaqMan real-time PCR for detecting infectious spleen and kidney necrosis virus in Siniperca chuatsi. Microbial Pathogenesis, 2017,107:98-105
Xia Luo, Xiaozhe Fu, Guoli Liao, Ouqin Chang, Zhibin Huang *, Ningqiu Li*. Isolation, pathogenicity and characterization of a novel bacterial pathogenStreptococcus uberisfrom diseased mandarin fishSiniperca chuatsi，Microbial Pathogenesis,2017, 107: 380-389
Hongru Liang, Xiaozhe Fu, Lihui Liu, Qiang Lin, Huizhi Guo,Yonggang Li, JiaXu Liu, Zhibin Huang, Ningqiu Li*. Inhibition of Grass Carp Reovirus Infection by DNA Aptamers against S10 Protein.Journal of Aquatic Animal Health, 2017, 29: 89–94.
Xiaozhe Fu, Qiang Lin, Hongru Liang, Lihui Liu, Zhibin Huang, Ningqiu Li *, Jianguo Su *. The biological features and genetic diversity of novel fish rhabdovirus isolates in China. Archives of Virology， 2017，162:2829–2834
Qiang Lin, Xiaozhe Fu, Ningqiu Li, Quanyuan Wan, Wenjie Chen,Yunmao Huang, Zhibin Huang, Jun Li, Lijuan Zhao* , Li Lin*. Co-infections of infectious spleen and kidney necrosis virus and Siniperca chuatsirhabdovirus in Chinese perch (Siniperca chuatsi) . Microbial Pathogenesis, 2017,111:422-430
Huizhi Guo, Xiaozhe Fu, Qiang Lin, Lihui Liu，Hongru Liang，Zhibin Huang, Ningqiu Li*, Jianguo Su*. Mandarin fish p53: genomic structure, alternatively spliced variant and its mRNA expression after virus challenge. Fish & Shellfish Immunology, 2017, 70：536-544
Xiaozhe Fu, Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang, Ningqiu Li*. The pathogenicity and biological features of Santee-Cooper Ranaviruses isolated from Chinese perch and snakehead fish. Microbial Pathogenesis, 2017，112：269–273
Lin Teng, Limei Deng, Xingxing Dong, Shun Wei, Jinquan Li, Ningqiu Li, Yang Zhou. Genome Sequence of Hypervirulent Aeromonas hydrophila Strain HZAUAH. Genome Announcements，2017，5 （11）： e00012-17
Xiaozhe Fu, Ningqiu Li*, Qiang Lin, Lihui Liu, Hongru Liang, Zhibin Huang. Display of ISKNV orf086 protein on the Surface of Aeromonas hydrophila and its immunogenicity in Chinese perch (Siniperca chuatsi). Fish & Shellfish Immunology, 2016,56：286-293
Huizhi Guo, Xiaozhe Fu, Ningqiu Li*, Qiang Lin, Lihui Liu，Shuqin Wu. Molecular characterization and expression pattern of tumor suppressor protein p53 in mandarin fish, Siniperca chuatsi following virus challenge. Fish & Shellfish Immunology,2016, 51:392-400
LIN Qiang (林强), LI Ningqiu (李宁求) * , FU Xiaozhe (付小哲) , HU Qiandong (胡钱东) ，CHANG Ouqin (常藕琴) ，LIU Lihui (刘礼辉) , ZHANG Defeng (张德锋) ，WANG Guangjun (王广军) ， SAN Guibao (伞桂宝) 。 WU Shuqin (吴淑勤) *. An outbreak of granulomatous inflammation associated with Francisell a noatunensissubsp. orientalisin farmed tilapia ( Oreochromis niloticus× O. aureus) in China。Chinese Journal of Oceanology and Limnology，2016,34( 3):460-466
Liu, Lihui; He, Jinzhao; Li, Ningqiu*; Fu, Xiaozhe; Lin, Qiang; Shi, Cunbin; Lv, Yejian; Zhang, Zhixin Complete mitochondrial genome of Parachromis managuensis (Perciformes: Cichlidae). Mitochondrial DNA, 2016，27（4）：2533-2534
Jiagang Tu, Wenjie Chen, Xiaozhe Fu, Qiang Lin, Ouqin Chang, Lijuan Zhao, Jiangfeng Lan, Ningqiu Li*,Li Lin*. Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection. International Journal of Molecular Sciences, 2016, 17, 740
Yong Liu, Ningqiu Li, Xinpeng Zhao, Bin Yue, Shuwen He, Zhixin Gao, Shun Zhou, Min Zhang*. A C-type lectin that inhibits bacterial infection and facilitates viral invasion in black rockfish，Sebastes schlegelii. Fish and Shellfish Immunology, 2016, 57:309–317.
Shu-wen He, Jian Zhang, Ning-qiu Li, Shun Zhou, Bin Yue, Min Zhang*. A TFPI-1 peptide that induces degradation of bacterial nucleic acids, and inhibits bacterial and viral infection in half-smooth tongue sole, Cynoglossus semilaevis. Fish and Shellfish Immunology, 2017，60：466-473
HongRu Liang, XiaoZhe Fu, NingQiu Li, LiHui Liu, Qiang Lin, YongGang Li, YongAo Peng, ShuQin Wu*. The distribution of different virulence grass carp reovirus strains in some neglected tissues. Polish Journal of Veterinary Sciences，2016. 19 （4）：763-770
Lihui Liu, Ningqiu Li*, Defeng Zhang, Xiaozhe Fu, Cunbin Shi, Qiang Lin. Complete Genome Sequence of the Type Strain of Aeromonas schubertii, ATCC 43700. Genome Announcements,2016, 4(1): e00012-16
Lihui Liu, Ningqiu Li, Defeng Zhang*, Xiaozhe Fu, Cunbin Shi, Qiang Lin, Guijie Hao*. Complete Genome Sequence of the Highly Virulent Aeromonas schubertii Strain WL1483, Isolated from Diseased Snakehead Fish (Channa argus) in China. Genome Announcements, 2016, 4(1) e01567-15
Wuming Yang, Ningqiu Li*,Ming Li, Defeng Zhang, Guannan An. A Complete Genome Sequence of Fish Pathogen Aeromonas hydrophila JBN2301. Genome Announcements, 2016, 4(1):e01615-15
Ningqiu Li*; Qiang Lin; Xiaozhe Fu; Huizhi Guo; Lihui Liu; Shuqin Wu*, Development and efficacy of a novel streptomycin-resistant Flavobacterium johnsoniae vaccine in grass carp (Ctenopharyngodon idella), Aquaculture, 448卷, pp 93-97, 2015/11/1.
Xiaozhe Fu; Ningqiu Li*; Qiang Lin; Huizhi Guo; Lihui Liu; Zhibin Huang; Shuqin Wu, Early protein ORF086 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in mandarin fish Siniperca chuatsi, Fish & Shellfish Immunology, 46(2), pp 200-205, 2015/10.
Xianqin Hu; Xiaozhe Fu; Ningqiu Li*; Xingxing Dong; Lijuan Zhao; Jiangfeng Lan; Wei Ji; Weidong Zhou; Taoshan Ai; Shuqin Wu; Li Lin*, Transcriptomic analysis of Mandarin fish brain cells infected with infectious spleen and kidney necrosis virus with an emphasis on retinoic acid-inducible gene 1-like receptors and apoptosis pathways, Fish & Shellfish Immunology, 45(2), pp 619-629, 2015/8.
Ningqiu Li; Xiaozhe Fu; Huizhi Guo; Qiang Lin; Lihui Liu; Defeng Zhang; Xiang Fang; Shuqin Wu*, Protein encoded by ORF093 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in Chinese perch Siniperca chuatsi, Fish & Shellfish Immunology, 42(1), pp 88-90, 2015/1.
Fu, X; Li, N; Lai, Y; Luo, X; Wang, Y; Shi, C; Huang, Z; Wu, S*; Su, J*, A novel fish cell line derived from the brain of Chinese perch Siniperca chuatsi: development and characterization., Journal of Fish Biology, 86(1), pp 32-45, 2015/1.
Fu, Xiaozhe; Li, Ningqiu; Lin, Qiang; Guo, Huizhi; Zhang, Defeng; Liu, Lihui; Wu, Shuqin, Protective immunity against infectious spleen and kidney necrosis virus induced by immunization with DNA plasmid containing mcp gene in Chinese perch Siniperca chuatsi, Fish & Shellfish Immunology, 40(1), pp 259-266, 2014/9.
Yang, Song; Wu, Shuqin; Li, Ningqiu; Shi, Cunbin; Deng, Guocheng; Wang, Qing; Zeng, Weiwei; Lin, Qiang, A cross-sectional study of the association between risk factors and hemorrhagic disease of grass carp in ponds in Southern China., Journal of Aquatic Animal Health, 25(4), pp 265-273, 2013/12.
Li Ningqiu; Fu Xiaozhe; Han Jingang; Shi Cunbin; Huang Zhibin; Wu Shuqin, Molecular cloning and expression of a heat-shock cognate 70 (hsc70) gene from swordtail fish (Xiphophorus helleri), Chinese Journal of Oceanology and Limnology, 31(4), pp 821-829, 2013/7.
Fu, Xiaozhe; Li, Ningqiu; Lai, Yingtiao; Liu, Lihui; Lin, Qiang; Shi, Cunbin; Huang, Zhibin; Wu, Shuqin, Protective immunity against iridovirus disease in mandarin fish, induced by recombinant major capsid protein of infectious spleen and kidney necrosis virus, Fish & Shellfish Immunology, 33(4), pp 880-885, 2012/10.
Fu, Xiaozhe; Li, Ningqiu; Liu, Lihui; Lin, Qiang; Wang, Fang; Lai, Yingtiao; Jiang, Haiming; Pan, Houjun; Shi, Cunbin; Wu, Shuqin, Genotype and host range analysis of infectious spleen and kidney necrosis virus (ISKNV), Virus Genes, 42(1), pp 97-109, 2011/2.
Li, Ningqiu; Yang, Zhihui; Bai, Junjie; Fu, Xiaozhe; Liu, Lihui; Shi, Cunbin; Wu, Shuqin, A shared antigen among Vibrio species: Outer membrane protein-OmpK as a versatile Vibriosis vaccine candidate in Orange-spotted grouper (Epinephelus coioides), Fish & Shellfish Immunology, 28(5-6), pp 952-956, 2010/6
Li Ningqiu; Bai Junjie; Wu Shuqin; Fu Xiaozhe; Lao Haihua; Ye Xing; Shi Cunbin, An outer membrane protein, OmpK, is an effective vaccine candidate for Vibrio harveyi in Orange-spotted grouper (Epinephelus coioides), Fish & Shellfish Immunology, 25(6), pp 829-833, 2008/12.
中文文章：
郭茜茜, 付小哲, 梁红茹, 林强, 刘礼辉, 牛银杰, 李宁求*。鳜弹状病毒调控谷氨酰胺还原性代谢途径促进自身增殖。中国水产科学，2019，26(5): 993-1003
刘礼辉，胡蕾，林强，付小哲，梁红茹，赵永亮，李宁求*。舒伯特气单胞菌荧光定量PCR检测方法的建立及应用。西北农林科技大学学报自然科学版，2019,47（8）:17-24
刘慧芳，李宁求 ，张莉娟，梁红茹 ，林强 ，刘礼辉，黄志斌，付小哲。弹状病毒反向遗传操作系统研究进展。基因组学与应用生物学，2019,38（3）:1144-1152
梁红茹,范芷仪,蔡秀珠,付小哲,林强,刘礼辉,黄志斌,牛银杰,林蠡,李宁求*.传染性脾肾坏死病毒与鳜弹状病毒双重PCR检测方法的建立. 西北农林科技大学学报自然科学版，2020,48(3):1-9
刘慧芳,张莉娟,李宁求,梁红茹,林强,刘礼辉,牛银杰,付小哲.加州鲈弹状病毒三水株全基因组序列测定及分析. 西北农林科技大学学报自然科学版，2020,48(3):1-9
梁红茹，蔡秀珠，范芷仪，林强，付小哲，刘礼辉，黄志斌，牛银杰，林蠡，李宁求*. 鳜弹状病毒TaqMan 荧光定量 PCR检测方法的建立及应用.中国预防兽医学报，2019，41（9）：929-934
胡蕾, 刘礼辉, 杨媛媛, 方伟, 林强，付小哲，梁红茹，刘山桂，李宁求*。杂交鳢舒伯特气单胞菌弱毒疫苗候选株筛选及其免疫效果评价. 淡水渔业，2019,49(5): 3-11
周阳，李本旺，林强，付小哲，李敏，梁红茹，刘礼辉，卢伟华，李宁求*。病毒灭活疫苗诱导云斑尖塘鳢脾脏转录组分析。基因组学与应用生物学，2019,10: 4404-4412
罗霞，付小哲， 李宁求*，林强，黄志斌，张悠。基于CPB细胞扩增体系的鳜弹状病毒增殖条件的优化。水产学报，2018,42（9）:1474-1481
张莉娟, 杨圆圆, 方伟, 李宁求, 梁红茹, 林强, 刘礼辉, 黄志斌, 付小哲. 弹状病毒基因组及功能基因研究进展. 基因组学与应用生物学，2018，37(2): 815-826
林强，李宁求*，石存斌，付小哲，刘礼辉，吴淑勤. 基于层次分析法的牡蛎养殖过程中副溶血弧菌风险评估. 海洋湖沼通报，2017，155（2）：116-122。
张醴溪，付小哲，林强，李趁，刘礼辉，梁红茹，黄志斌，吴志新，李宁求*．基于病毒mRNA监测的传染性脾肾坏死病毒灭活快速检验方法建立及其应用．水产学报，2017，41（9）：1464-1472
胡松, 李宁求*, 付小哲, 林强, 刘礼辉, 梁红茹. 鳜传染性脾肾坏死病毒研究进展. 基因组学与应用生物学，2017，36（3）：976-984
付小哲，方伟，林强，刘礼辉，梁红茹，黄志斌，李宁求*。翘嘴鳜对约氏黄杆菌减毒活疫苗免疫应答. 南方水产科学, 2017，13（5）：33-38
梁红茹,付小哲.林强.刘礼辉.李宁求*.草鱼呼肠孤病毒S10蛋白的适配体筛选及对病毒抑制研究.珠江水产科学.2017,25(2):1-7.
张醴溪,付小哲,林强,刘礼辉,梁红茹,黄志斌,李宁求* .基于跨内含子设计引物检测病毒mRNA的传染性脾肾坏死病毒灭活快速检测方法, 珠江水产科学,2017,25（4）:24-33.
林强，付小哲，刘礼辉，梁红茹，李宁求*. 水产养殖中副溶血弧菌风险评估技术研究进展, 珠江水产科学,2017,25(2):94-100
杨圆圆，方伟，林汉群，刘礼辉，李宁求。大鲵虹彩病毒引起的溃疡病。海洋与渔业，2017,（4）:52-53
付小哲，李宁求*，林强，刘礼辉，吴淑勤。鳜传染性脾肾坏死病毒主衣壳蛋白单克隆抗体的制备及鉴定。水产学报，2016,40(3):363-370
林强，杨淞, 李宁求*, 方翔，付小哲，刘礼辉，郭慧芝，李万里，吴淑勤。IRAK4基因的克隆、组织表达及病毒感染后表达分析。水产学报，2016,40(3):344-354
吴仕伟，付小哲，林强，刘礼辉，梁红茹，黄志斌，李宁求*。病毒感染影响宿主细胞葡萄糖代谢研究进展，病毒学报，2016,32（6）:800-809
刘礼辉，张德锋，李宁求，付小哲，林强 ，石存斌*，斑点叉尾鮰败血三种病原多重PCR检测方法的建立与应用，西北农林科技大学学报自然科学版，2016,44(11):39-46
梁红茹，付小哲，李宁求，林强，刘礼辉，方翔，黄志斌，吴淑勤*. 鱼类弹状病毒病的研究进展. 珠江水产科学. 2016，22（2）：59-63
胡先勤，付小哲， 董星星，林强，涂加钢，赵丽娟，李宁求*，林蠡*。鳜鱼亲环蛋白A在传染性脾肾坏死病毒增殖中的作用，水产学报，2016,40(1):100-109
钟 蕾，向建国*，曾丹，李宁求。饵料对鳡肠道微生物多样性的影响，水生生物学报，2016,40（4）:830-835
付天增, 郝金婷, 李宁求, 闫茂仓, 李煜, 吴佳燕, 王雪鹏*。迟缓爱德华菌菌蜕疫苗对罗非鱼注射和口服免疫的效果分析。水生生物学报，2016,40（6）：158-165
张德锋；刘礼辉；任燕；李宁求；林强；潘厚军；石存斌；吴淑勤*, 我国罗非鱼源新型无乳链球菌的分离、鉴定及其分子特征, 中国水产科学, 05期, pp 1044-1054, 2015/9/15.
张德锋，刘礼辉，李宁求，任燕，林强，石存斌，吴淑勤*。我国南方地区鱼源气单胞菌不同种类的流行特征。水产科学，2015,34（11）：673-682
张德锋, 林强, 刘礼辉, 任燕, 李宁求, 付小哲, 石存斌, 吴淑勤*. 美国红鱼水生气单胞菌的分离、鉴定及其分子特征. 淡水渔业. 2015, 45(6): 61-68
时云朵；任燕；张德锋；付小哲；林强；李宁求；石存斌；吴淑勤*, 山莨菪碱提高嗜水气单胞菌灭活疫苗浸泡免疫鲫的效果, 水产学报, 05期, pp 720-727, 2015/5/15.
方翔；李宁求*；付小哲；李凯彬；林强；刘礼辉；石存斌；吴淑勤*, 基于“天河二号”的水产病原生物信息分析平台构建及其在水产病原分析中的应用, 遗传, 07期, pp 702-710, 2015/5.
王敏；李宁求；闫茂仓；常维山；郝金婷；庞晓茹；王雪鹏*, 迟缓爱德华菌luxS基因在不同生长时期表达水平的差异, 微生物学报, 09期, pp 1201-1207, 2015/2.
罗霞，付小哲，李宁求*，林强，张悠，黄志斌. 同步接毒条件下鳜传染性脾肾坏死病毒在CPB细胞中的最适增殖条件. 水产学报，2015,39（10）:162-170
任燕；张小江；陶家发；李宁求；石存斌；吴淑勤*, 壳聚糖-海藻酸盐复合微囊疫苗的制备及其对斜带石斑鱼的口服免疫效果, 中国生物制品学杂志, 02期, pp 110-114, 2015.
任燕；张德峰；孙承文；陶家发；李宁求；刘礼辉；石存斌；吴淑勤*, 鱼源嗜水气单胞菌疫苗生产菌株与分离株的抗原性分析, 中国预防兽医学报, 03期, pp 207-210, 2015.
李宁求；余露军；付小哲；刘礼辉；林强；张德锋；石存斌；吴淑勤*, 3种免疫途径下迟缓爱德华菌菌蜕疫苗对欧洲鳗的免疫保护效果, 水产学报, 11期, pp 1910-1916, 2014/11/15.
付小哲；李宁求；林强；石存斌；吴淑勤*, 基于荧光定量PCR的鳜传染性脾肾坏死病毒滴度检测方法, 水产学报, 09期, pp 1573-1578, 2014/9/15.
刘礼辉；石存斌；李宁求；付小哲；林强, 鱼类药饵科学使用策略, 科学养鱼, 07期, p 70, 2014/7/10.
李宁求；米彦飞；付小哲；林强；石存斌；邓国成；吴淑勤*, 影响草鱼出血病疫苗免疫效果因素风险评估, 中国水产科学, 2014/3.
胡钱东；林强；石存斌；付小哲；李宁求；刘礼辉；吴淑勤*, 约氏黄杆菌研究进展, 生命科学研究, 01期, pp 60-65, 2014/2/28.
郭慧芝；刘礼辉；李宁求；付小哲；石存斌, 草鱼疾病流行规律及防治措施, 科学养鱼, 02期, pp 59-60, 2014.
胡钱东；林强；石存斌；付小哲；李宁求；刘礼辉；吴淑勤*, 草鱼致病性类志贺邻单胞菌的分离与鉴定, 微生物学报, 02期, pp 229-235, 2014.
付小哲；方翔；李宁求；林强；潘厚军；石存斌；黄志斌；吴淑勤*, 鳜鱼嗜水气单胞菌GYK1株外膜蛋白A基因的克隆及其生物信息学分析, 中国生物制品学杂志, 09期, pp 1222-1227, 2013/9/20.
米彦飞；李宁求；付小哲；巩华；石存斌；吴淑勤*, 草鱼疫苗接种成本-效益分析, 广东农业科学, 16期, pp 116-119, 2013/8/25.
付小哲；李宁求；彭媛媛；林强；石存斌；黄志斌；吴淑勤*, 鳜传染性脾肾坏死病毒ORF093基因的克隆、表达及其重组蛋白的免疫原性分析, 中国水产科学, 02期, pp 427-433, 2013/3/15.
李宁求；付小哲；赖迎迢；王亚军；任燕；石存斌；吴淑勤*, 水产立体养殖模式传播禽流感的风险分析, 动物医学进展, 12期, pp 186-189, 2013.
杨淞；吴淑勤*；李宁求；石存斌；邓国成；林文辉；林强, 草鱼出血病发生风险半定量评估模型的构建, 中国水产科学, 03期, pp 521-527, 2012.
杨淞；李宁求；石存斌；吴淑勤*, 风险分析在水生动物健康管理上的研究进展, 江苏农业科学, 01期, pp 195-199, 2012.
陶家发；赖迎迢；孙承文；任燕；李宁求；石存斌, 弧菌外膜蛋白ompk工程菌规模化培养表达初探, 生物技术, 04期, pp 72-75, 2012.
林强；李宁求；付小哲；刘礼辉；石存斌；吴淑勤*, 牡蛎养殖过程中副溶血弧菌与水质因子间的关系, 水产学报, 03期, pp 415-421, 2012.
李宁求；余露军；付小哲；刘礼辉；林强；常藕琴；石存斌；黄志斌；吴淑勤*, 鳗源迟缓爱德华氏菌菌蜕的构建及制备条件优化, 水产学报, 11期, pp 1754-1762, 2012.
林强；李宁求；付小哲；刘礼辉；石存斌；吴淑勤*, 牡蛎中副溶血弧菌荧光定量PCR检测方法的建立及其应用, 中国水产科学, 01期, pp 96-102, 2011.
李宁求；付小哲；石存斌；吴淑勤*, 大宗淡水鱼类病害防控技术现状及前景展望, 动物医学进展, 04期, pp 113-117, 2011.
刘礼辉；李宁求；石存斌；吴淑勤*, TAIL-PCR方法克隆约氏黄杆菌aroA基因序列, 安徽农业科学, 32期, 2010/11/10.
刘礼辉；李宁求；石存斌；吴淑勤*, TAIL-PCR方法克隆约氏黄杆菌aroA基因序列(摘要)(英文), Agricultural Science & Technology, 07期, pp 179-182, 2010/7/15.
余露军；李宁求；刘礼辉；付小哲；石存斌；潘厚军；吴淑勤*, 日本鳗鲡混合感染迟缓爱德华氏菌与创伤弧菌的分离与鉴定, 中国人兽共患病学报, 08期, 2009/8/15.
付小哲；李宁求；彭媛媛；石存斌；白俊杰；吴淑勤*, 鳜传染性脾肾坏死病毒重组主衣壳蛋白免疫效果的初步验证, 中国水产科学, 03期, 2009/5/15.
彭媛媛；付小哲；石存斌；余露军；李宁求；吴淑勤*, 传染性脾肾坏死病毒(ISKNV)ORF086基因的表达·纯化及其抗体的制备, 安徽农业科学, 11期, 2009/4/10.
张小江；任燕；常藕琴；石存斌；李宁求；吴淑勤*, 斜带石斑鱼口服PELA-OmpK微球疫苗的示踪及免疫效果, 中国水产科学, 05期, 2008/9/15.
刘礼辉；李宁求；石存斌；潘厚军；付小哲；吴淑勤*, 斑点叉尾鮰烂鳃病病原柱状黄杆菌的分离及鉴定, 安徽农业科学, 17期, 2008/6/10.
付小哲；石存斌；李宁求；潘厚军；常藕琴；吴淑勤*, 军曹鱼淋巴囊肿病毒主衣壳蛋白基因全序列分析, 病毒学报, 05期, pp 412-416, 2007/9/15.
杨智慧；李宁求；白俊杰；石存斌；潘厚军；叶星；劳海华；吴淑勤*, 19株海水鱼致病性弧菌OmpK基因序列及其抗原性分析, 中国水产科学, 05期, pp 807-813, 2006/9/30.
吴淑勤*；李宁求；李凯彬；巩华；黄志斌；潘厚军；张敏, 水生生物在人体公共卫生研究中应用的思考, 中国比较医学杂志, 06期, pp 373-375+378, 2006/6/30.
李宁求；白俊杰；吴淑勤*；劳海华；石存斌；潘厚军；叶星；简清, 斜带石斑鱼3种致病性弧菌的分子生物学鉴定, 水产学报, 03期, pp 356-361, 2005/6/30.
潘厚军；吴淑勤*；李宁求；石存斌；李凯彬；巩华；陶家发；常藕琴, 草鱼肠道中香港海鸥型菌的选择性分离与鉴定, 中国水产科学, 03期, pp 307-313, 2005/6/30.
胡学峰；石存斌；潘厚军；李宁求；吴淑勤*, 海水养殖斜带石斑鱼溃疡病病原菌(溶藻弧菌)的初步研究, 中国海洋大学学报(自然科学版), 02期, pp 232-236, 2005/3/30.
陈献稿；吴淑勤*；石存斌；李宁求, 斜带石斑鱼病原菌(哈维氏弧菌)的分离与鉴定, 中国水产科学, 04期, pp 313-317, 2004/8/30.