吴辉

生物工程专业：博士生导师/硕士生导师
生物化工专业：博士生导师/硕士生导师
生物工程领域：工程硕士导师

吴辉，工学博士，副教授，博导。现任华东理工大学教务处副处长兼本科生招生办公室主任、中国轻工业生物基材料工程重点实验室副主任，中国化工学会生物化工专委会青年****工作委员会委员，中国生物工程学会青年工作委员会委员，上海市生物工程学会合成生物学委员会委员，生物反应器工程国家重点实验室固定人员。教育部“霍英东青年教师基金”获得者（2018）。2019年被破格遴选为博士生导师。
2004年毕业于南京工业大学生物工程系，获工学学士学位。2009年毕业于华东理工大学发酵工程专业，获工学博士学位。同年进入中科院上海生命科学研究院植物生理生态研究所从事博士后研究工作。2011年来到誉有“南方的哈佛”之称的美国莱斯大学生物工程系从事博士后研究工作。2014年9月引进华东理工大学生物工程学院，入选华东理工大学“青年英才培育计划”（2018 A类、2015）等。
作为负责人主持国家自然科学基金面上项目、青年项目、教育部“霍英东青年教师基金” 、上海市自然科学基金等项目；作为主要研究骨干参与科技部国家重点研发计划课题、863计划等。近年来以第一作者或通讯作者在Metabolic Engineering，Biotechnology Advances，ACS Synthetic Biology，Biotechnology and Bioengineering等本领域重点期刊上发表SCI收录论文近40篇。申请美国发明专利6项，已授权3项；申请中国发明专利11项，已授权5项；申请实用新型专利1 项。获2019年度华东理工大学优秀研究生指导教师。指导研究生多次获得校外奖学金，华东理工大学第十三届“华陆科技· 奋进杯”大学生课外学术科技作品竞赛二等奖；共同指导并带队本科生获2017年和2018年连续两次获得国际遗传工程机器大赛(iGEM)竞赛金奖，指导“大学生创新创业训练计划”上海市级和校级项目。
研究方向：
1．微生物代谢工程生产平台化合物和生物活性物质
运用生物信息学及代谢工程的最新技术和方法，合理设计及重构微生物代谢途径，结合代谢调控策略，建立以可再生生物质资源（糖、甘油、乙酸、甲酸、CO₂等）为原料的新型高效微生物细胞工厂，合成多种生物基化学品与生物基材料，如丁二酸，3-羟基丙酸，丙酮，异丙醇，生物聚酯，氨基酸，脂肪酸及其衍生物等。
2．合成生物学中“代谢晶体管”研究及其应用
“代谢晶体管”控制模式借鉴电子元器件“晶体管”的放大控制模式，通过精细调节细胞中微量关键物质的浓度，进而对整个细胞进行代谢重编程。
3. 基于微生物电化学合成的代谢工程与合成生物学
运用代谢工程与合成生物学的策略，结合电化学平台技术与方法，搭建能利用一碳资源的自养型大肠杆菌细胞工厂平台，用于生产多种生物基化合物如丙酮，异丙醇，3-羟基丙酸，丁二酸等。
主持和参加的科研项目：
1．主持国家自然科学基金面上基金2018.1-2021.12
2．主持霍英东教育基金会第十六届高等院校青年教师基金2018.3-2021.3
3．主持上海市自然科学基金2019.6-2022.6
4．主持中科院合成生物学重点实验室开放课题2020.6-2022.6
5．主持国家自然科学基金青年科学基金2015.1-2017.12
6．主持生工学院优秀青年教师“远志计划”2017.1-2018.12
7．主持工业生物催化教育部重点实验室开放课题2015.12-2017.12
8．主持中央高校基本科研业务费探索研究基金 2015.7-2017.6
9．主持生工学院优秀青年教师培育计划，2015.07-2017.07
10．参与国家重点研发计划（2017YFB**）2017.07 - 2021.06
11．参与上海市“科技创新行动计划” 2013.9-2016.9
12．参与国家863计划“基因组规模系统代谢育种” 2012.11-2015.12
13．参与国家863计划“化学品生物转化过程与装备” 2012.11-2015.12
学术与社会兼职：
Frontiers in Microbiology, SCI收录，副主编（2019-）
Frontiers in Bioengineering and Biotechnology, SCI收录，编委（2016-）
Frontiers in Bioengineering and Biotechnology, SCI收录，客座副主编（2019,2020）
Bioresources and Bioprocessing, ESCI收录，青年编委（2020-）
Bioresources and Bioprocessing, ESCI收录，执行编辑（2016-2019）
Bioresources and Bioprocessing, ESCI收录，客座主编（2015-2016）
美国微生物学会（ASM）会员（2015-）
美国工业微生物与生物技术学会（SIMB）会员（2013-）
中国化工学会生物化工专委会青年****工作委员会委员（2017-2021）
中国生物工程学会青年工作委员会委员（2019-2023）
上海市生物工程学会合成生物学委员会委员（2018-2022）
多个国际杂志如Biotechnology Advances，Metabolic Engineering，Biotechnology and Bioengineering，Biotechnology for Biofuel，Scientific Reports，Microbial Cell Factories，Frontiers in Microbiology，Journal of Agricultural and Food Chemistry，Bioresource Technology，Biotechnology Journal，Biochemical Engineering Journal，Process Biochemistry，Bioprocess and Biosystems Engineering，Bioresources and Bioprocessing，Frontiers in Bioengineering and Biotechnology，Applied Biochemistry and Biotechnology，Biotechnology and Applied Biochemistry等审稿人。多个国内杂志《生物工程学报》、《微生物学报》、《生物加工过程》等期刊审稿人。
本科和研究生教学：
1.本科专业必修课，微生物（全英文），48学时，3学分；
2.博士研究生专业课程，系统代谢工程（与花强教授合作），32学时，2学分；
3. iGEM竞赛导师，Bio-maker创新活动指导老师，大创指导老师。
代表性论文（*通讯作者）：
1. Guohui Li, Dixuan Huang, Xue Sui, Shiyun Li, Bing Huang, Xiaojuan Zhang*, Hui Wu*, Yu Deng*. Advances in microbial production of medium-chain dicarboxylic acids for nylon materials. Reaction Chemistry & Engineering. 2020. 5:221-238 (IF=3.441, JCR Q2)
2. Hao Yang, Can Zhang, Ningyu Lai, Bing Huang, Peng Fei, Dawei Ding, Peng Hu, Yang Gu, Hui Wu*. Efficient isopropanol biosynthesis by engineered Escherichia coli using biologically produced acetate from syngas fermentation. Bioresource Technology. 2020. 296:122337.(IF=7.53, JCR Q1)
3．Qiaofei He, George N. Bennett, Ka-Yiu San, Hui Wu*.Biosynthesis of medium-chain ω-hydroxy fatty acids by AlkBGT of Pseudomonas putidaGPo1 with native FadL in engineered Escherichia coli. Frontiers in Bioengineering and Biotechnology. 2019. 7:273.(IF=3.644, JCR Q2)
4．Hao Yang^#, Bing Huang^#, Ningyu Lai, Yang Gu, Zhimin Li, Qin Ye, Hui Wu*. Metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate. Microbial Cell Factories.2019. 18:6.(IF=4.187, JCR Q1)
5. Jingxian Lu, Zhimin Li, Qin Ye, Hui Wu*. Effect of reducing the activity of respiratory chain on biosynthesis of poly(3-hydroxybutyrate-co-lactate) in Escherichia coli. Chinese Journal of Biotechnology.2019. 35(1):59-69.
6．Jiapeng Tang, Zhenqing Qian, Hui Wu*. Enhancing cordycepin production in liquid static cultivation of Cordyceps militarisby adding vegetable oils as the secondary carbon source. Bioresource Technology. 2018. 268:60-67. (IF=7.53, JCR Q1)
7．Bing Huang, Hao Yang, Guochen Fang, Xing Zhang, Hui Wu*, Zhimin Li*, Qin Ye. Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli. Biotechnology and Bioengineering. 2018. 115:943-954. (*Co-corresponding)(IF=4.00, JCR Q1)
8．WeiLi^#, Hui Wu^#, Mai Li, Ka-Yiu San*. Effect of NADPH availability on free fatty acid production inE. coli. Biotechnology and Bioengineering. 2018. 115:444-452. (^#First two authors contributed to this paper equally)(IF=4.00, JCR Q1)
9．Han Liu, Guochen Fang, Hui Wu*, Zhimin Li*,Qin Ye. L-cysteine production inEscherichia colibased on rational metabolic engineering and modular strategy. Biotechnology Journal. 2018. 13:e**. (*Co-corresponding)(IF=3.91, JCR Q1)
10．Qing Li#, Bing Huang#, Qiaofei He, Jingxian Lu, Xun Li, Zhimin Li, Hui Wu*, Qin Ye. Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation.Bioresources and Bioprocessing. 2018. 5:41. (^#First two authors contributed to this paper equally) (Feature Article)
11. Jiaqi Jiang, Bing Huang, Hui Wu*, Zhimin Li*, Qin Ye. Efficient 3-hydroxypropionic acid production from glycerol by metabolically engineered Klebsiella pneumoniae. Bioresources and Bioprocessing.2018. 5:34. (*Co-corresponding)
12．Qing Li, Bing Huang, Hui Wu*, Zhimin Li, Qin Ye. Efficient anaerobic production of succinate from glycerol in engineered Escherichia coli by using dual carbon sources and limiting oxygen supply in preceding aerobic culture. Bioresource Technology. 2017. 231:75-84.(IF=7.53, JCR Q1)
13．Xing Zhang^#, Hui Wu^#, Bing Huang, Zhimin Li*, Qin Ye. One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system. Journal of Biotechnology. 2017. 241:163-169. (^#First two authors contributed to this paper equally)(IF=3.50, JCR Q2)
14．Qing Li^#, Hui Wu^#, Zhimin Li, Qin Ye*. Enhanced succinate production from glycerol by engineered Escherichia colistrains. Bioresource Technology. 2016. 218:217-223. (^#First two authors contributed to this paper equally)(IF=7.53, JCR Q1)
15．Yunjie Li, Bing Huang, Hui Wu*,Zhimin Li*, Qin Ye, Yi-Heng Percival Zhang. Production of succinate from acetate by metabolically engineeredEscherichia coli. ACS Synthetic Biology. 2016. 5:1299-1307. (*Co-corresponding)(IF=4.41, JCR Q1)
16．Jianhua Yang, Wei Li, Dezheng Wang, Hui Wu*,Zhimin Li*, Qin Ye. Characterization of bifunctional L-glutathione synthetases from Actinobacillus pleuropneumoniaeand Actinobacillus succinogenesfor efficient glutathione biosynthesis. Applied Microbiology and Biotechnology. 2016. 100(14):6279-6289. (*Co-corresponding)(IF=3.53, JCR Q2)
17．Jing Zhang, Cong Quan, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. Systematic manipulation of glutathione metabolism in Escherichia colifor improved glutathione production. Microbial Cell Factories.2016. 15:38.(*Co-corresponding) (IF=4.18, JCR Q1)
18．Dezheng Wang, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. Glutathione production by recombinant Escherichia coliexpressing bifunctional glutathione synthetase. Journal of Industrial Microbiology and Biotechnology.2016. 43:45-53.(*Co-corresponding)(IF=2.824, JCR Q2)
19．Cheng Wang, Jing Zhang, Hui Wu*, Zhimin Li*, Qin Ye. Heterologous gshFgene expression in various vector systems in Escherichia coli for enhanced glutathione production. Journal of Biotechnology. 2015. 214:63-68. (*Co-corresponding).(IF=3.50, JCR Q2)
20．Hui Wu, Leepika Tuli, George N. Bennett, Ka-Yiu San*. Metabolic transistor strategy for controlling electron transfer chain in Escherichia coli.Metabolic Engineering. 2015. 28:159-168. (IF=7.26, JCR Q1)
21．Hui Wu,George N. Bennett, Ka-Yiu San*. Metabolic control of respiratory levels in coenzyme Q biosynthesis-deficient Escherichia coli strains leading to fine-tune aerobic lactate fermentation. Biotechnology and Bioengineering. 2015. 112(8):1720-1726.(IF=4.00, JCR Q1)
22．Hui Wu, Jane Lee, Mukund Karanjikar, Ka-Yiu San*. Simultaneous utilization of glucose and mannose from woody hydrolysate for free fatty acid production by metabolically engineered Escherichia coli. Bioresource Technology. 2015. 185:431-435.(IF=7.53, JCR Q1)
23．Hui Wu,Mukund Karanjikar, Ka-Yiu San*. Metabolic engineering of Escherichia colifor efficient free fatty acid production from glycerol. Metabolic Engineering. 2014. 25:82-91. (IF=7.26, JCR Q1)
24．Hui Wu, Ka-Yiu San*. Efficient odd numbered straight-chain free fatty acid production by metabolically engineered Escherichia coli. Biotechnology and Bioengineering. 2014. 111(11):2209-2219.(IF=4.00, JCR Q1)
25．Yane Luo*, Tao Zhang, Hui Wu*. The transport and mediation mechanisms of the common sugars in Escherichia coli. Biotechnology Advances. 2014. 32:905-919.(IF=10.74, JCR Q1)
26．Hui Wu, Jane Lee, Mukund Karanjikar, Ka-Yiu San*. Efficient free fatty acid production from woody biomass hydrolysate in metabolically engineered Escherichia coli. Bioresource Technology. 2014. 169:119-125.(IF=7.53, JCR Q1)
27．Hui Wu, Ka-Yiu San*. Engineering Escherichia colifor odd straight medium chain free fatty acid production. Applied Microbiology and Biotechnology.2014. 98(19):8145-8154.(IF=3.53, JCR Q2)
28．Hui Wu,Qing Li, Zhi-min Li*, Qin Ye*. Succinic acid production and CO₂fixation using a metabolically engineered Escherichia coliin a bioreactor equipped with a self-inducing agitator. Bioresource Technology. 2012. 107:376-384.(IF=7.53, JCR Q1)
29．Yuan Liu^?, Hui Wu^?, Qin Li, Xuwei Tang, Zhi-min Li*, Qin Ye. Process development of succinic acid production byEscherichia coli NZN111 using acetate as an aerobic carbon source. Enzyme and Microbial Technology.2011. 49(5):459-64. (^?First two authors contributed to this paper equally) (IF=3.45, JCR Q2)
30．Hui Wu, Zhi-min Li, Li Zhou, Jingli Xie, Qin Ye*. Enhanced anaerobic succinic acid production by Escherichia coliNZN111 aerobically grown on gluconeogenetic carbon sources. Enzyme and Microbial Technology. 2009. 44:165-169. (IF=3.45, JCR Q2)
31．Hui Wu, Zhi-minLi, Li Zhou, Qin Ye*. Improved succinic acid production in the anaerobic culture of an Escherichia coli pflB ldhA double mutant as a result of enhanced anaplerotic activities in the preceding aerobic culture. Applied and Environmental Microbiology. 2007. 73:7837-7843. (IF=4.02, JCR Q1)

其他合作发表文章：
1. Haritha Meruvu, Hui Wu, Ziyue Jiao, Liyan Wang, Qiang Fei, From Nature to Nurture: Essence and methods to isolate robust methanotrophic bacteria, Synthetic and Systems Biotechnology, 2020. 5(3):173-178. September
2．Yunpeng Yang^#, Nannan Lang^#, Lu Zhang, Hui Wu, Weihong Jiang*, Gu Yang*. A novel regulatory pathway consisting of a two-component system and an ABC-2 type transporter controls butanol tolerance inClostridium acetobutylicum. Applied Microbiology and Biotechnology. 2020. 104(11):5011-5023(IF=3.53, JCR Q2)
3．Jiawei Li, Xiaoyan Zhang, Hui Wu, Yunpeng Bai*. Transcription factor screening and engineering for metabolic engineering and production of organic acids: an overview. Frontiers in Bioengineering and Biotechnology. 2020. 8:98 (IF=3.644, JCR Q2)
4．Boliang Gao, Li Li, Hui Wu, Du Zhu, Min Jin, Wu Qu, Runying Zeng*. A novel strategy for efficient agaro-oligosaccharide production based on the enzymatic degradation of crude agarose in Flammeovirga pacifica WPAGA1. Frontiers in Microbiology.2019. 10:1231.(IF=4.235,JCR Q1)
5．Xiangwei Cui, Junxian Wan, Xing Zhang, Hui Wu, Zhimin Li*, Qin Ye. Efficient glutathione production in metabolically engineered Escherichia colistrains using constitutive promoters. Journal of Biotechnology. 2019. 289:39-45(IF=3.50, JCR Q2)
6．Bing Huang, Guocheng Fang, Hui Wu, Jibin Sun, Zhimin Li*, Qin Ye. Efficient biosynthesis of succinate from paper mill wastewater by engineered Escherichia coli. Applied Biochemistry and Biotechnology.2019. 189:1195-1208. (IF=2.14, JCR Q3)
7. Feng Li^#, Yuanxiu Li^#, Liming Sun, Xiaoli Chen, Xingjuan An, Changji Yin, Yingxiu Cao,Hui Wu, Hao Song*. Modular engineering intracellular NADH regeneration boosts extracellular electron transfer of Shewanella oneidensisMR-1. ACS Synthetic Biology. 2018. 7(3):885-895. (IF=4.41, JCR Q1)
8．Zhuan Cheng, Jiaqi Jiang, Hui Wu, Zhimin Li*, Qin Ye. Enhanced production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway by engineered Escherichia coli. Bioresource Technology.2016.200:897-904. (IF=7.53, JCR Q1)
9．Dan Wang, Hui Wu, Chandresh Thakker, Jared Beyersdorf, George N Bennett, Ka-Yiu San*. Efficient free fatty acid production in engineered Escherichia colistrains using soybean oligosaccharides as feedstock. Biotechnology Progress. 2015. 31:686-694. (IF=2.334, JCR Q2)
10．YangGu, Yu Jiang,Hui Wu, Xudong Liu, Zhilin Li, Jian Li, Han Xiao, Zhaobing Shen, Hongjun Dong, Yunliu Yang, Yin Li, Weihong Jiang*, Sheng Yang*. Economical challenges to microbial producers of butanol: feedstock, butanol ratio and titer. Biotechnology Journal. 2011. 6(11):1348-1357.(IF=3.91, JCR Q1)

撰写著作：
1. Qiang Fei, Haritha Meruvu, Hui Wu, Rongzhan Fu，Chapter 12: Biological Conversion of Natural Gas in Direct Natural Gas Conversion to Value-Added Chemicals, 512 Pages, ISBN 38, 2020, CRC Press.
2. Qin Ye*, Zhimin Li, Hui Wu. Principle and performance of gas self-inducing reactors and applications to biotechnology. Bioreactor Engineering Research and Industrial Applications II, Advances in Biochemical Engineering/Biotechnology, Volume 152, 2016. ISBN: 978-3-662-48346-6 (Print), 978-3-662-48347-3 (eBook). Springer, Berlin, Heidelberg, Germany.
发明专利：
1．Ka-Yiu San, George N. Bennett, Hui Wu. Metabolic transistors in bacteria. US Patent Number: 10,011,839 B2.
2．Ka-Yiu San, Xixian Xie, Leepika Tuli,Hui Wu. Hydroxy- and dicarboxylic-fat synthesis by microbes.US patent Number: 9,487,804 B2.
3．Ka-Yiu San, George N. Bennett, Hui Wu. Metabolic transistors in bacteria. US Patent Number: 9,441,253 B2.
4．Ka-Yiu San, Xian Zhang, Hui Wu, Dan Wang. KAS III-free fatty acid synthesis.US Patent. Publication Number: 2018/** A1.
5．Ka-YiuSan and Hui Wu. Integrated biodiesel process.US patent. Publication Number: 2014/** A1.
6．Ka-Yiu San and Hui Wu. Microbial odd chain fatty acids. US patent. Publication Number: 2014/** A1.
7. 吴辉，魏香菊，吴榉，郭鹏业. 一种提高聚羟基丁酸乳酸酯中乳酸组分含量的基因工程大肠杆菌的构建方法及应用.专利申请号:6.9.
8. 吴辉，赖宁玉，赵舒欣，王文卓，黎冠忠. 利用乙酸生产3-羟基丙酸的代谢工程大肠杆菌菌株的构建方法及应用.专利申请号:3.8.
9．吴辉，陆静娴，李志敏，叶勤. 一种提高大肠杆菌合成的聚（3-羟基丁酸-co-乳酸）中乳酸组分含量的方法.专利申请号:8.X.
10. 陆健，谭高翼，王启要，吴辉，夏建业，李友元，李晔，李媛媛. 一种用于光合生物反应器的发光搅拌桨. 专利公开(公告)号：CNU
11．吴辉，杨昊，李志敏，黄兵，叶勤. 利用乙酸生产丙酮或异丙醇的代谢工程大肠杆菌菌株的构建方法与应用. 专利申请号: 4.9.
12．吴辉，黄兵，李志敏，杨昊，叶勤. 利用乙酸生产羟基丙酸的代谢工程大肠杆菌菌株的构建方法与应用. 专利申请号: 3.0.
13．李志敏, 黄兵, 吴辉, 房国辰, 叶勤.一种利用造纸废液生物合成丁二酸的方法. 专利申请号: 7.6.
14．叶勤，李晴，李志敏，吴辉. 利用重组大肠杆菌以甘油为碳源发酵生产琥珀酸的方法. ZL2.6.
15．李志敏, 李运杰, 吴辉, 叶勤. 利用乙酸生产丁二酸的代谢工程大肠杆菌菌株构建方法和应用. ZL2.1.
16．孙周通，刘映淼，孙兵兵，王祎，吴辉，杨俊杰等. L-色氨酸基因工程菌,其构建方法以及使用其发酵生产L-色氨酸的方法. ZL0.
17．叶勤，李志敏，吴辉. 利用四碳二元酸发酵固定二氧化碳的方法. ZL4.
18．吴辉，李志敏，叶勤. 大肠杆菌生产琥珀酸的方法. ZL4.
联系方式：
上海市梅陇路130号283信箱邮编:200237
办公室：实验八楼415室
实验室：实验八楼404室
电话：
E-mail: hwu@ecust.edu.cn