万民熙


生物工程专业：硕士生导师

1981年9月生，湖南衡阳人，中美联合培养博士，生物工程专业硕士生导师。入选2018年云南省青年****（柔性引才）、2020年度华东理工大学生物工程学院“青年交叉创新提升计划（青椒计划）”、2015年度华东理工大学青年英才引进与培育计划。
近十年来，一直从事微生物选育、生理生化和分子生物学相关基础研究。针对微藻代谢产物的合成与基因遗传改造进行了大量研究工作，涉及生理生化、细胞生物学、分子生物学、代谢调控以及功能基因组学等方面的相关工作。通过全转录组测序、细胞代谢过程的碳流向解析、环境刺激与细胞互作机制研究等多种手段找到了能源微藻基因工程改造的关键基因。目前正在从事雨生红球藻高产虾青素的工艺开发和基因工程改造等研究工作。作为项目负责人承担国家高技术研究发展计划（863）课题、国家自然科学基金、中国博士后科学基金特别资助和一等资助、中央高校基本科研业务费-探索研究基金、973项目子课题等多个国家级/省部级项目。现已在生物技术领域相关期刊上公开发表SCI等各类论文43篇；申请发明专利12项，其中中国专利6项、PCT专利1项、美国/欧盟/澳大利亚/巴西/印度专利各1项。担任科技核心期刊《现代生物医学进展》编委，以及Bioresource Technology、Algae Research等国际期刊审稿人。
一、教育经历
2012/7 - 2014/11，华东理工大学，生物工程学院，博士后
2007/9 - 2012/6，中南大学，微生物系，博士
2010/1 - 2012/1，Johns Hopkins University，Department of Chemical and Biomolecular Engineering，联合培养博士
2004/9 - 2007/7，中南大学，生物工程系，硕士
1999/9 - 2003/7，中南大学，化学工程系，学士
二、工作经历
2019/8 - 至今，华东理工大学，生物工程学院，副教授
2016/7 - 至今，华东理工大学，生物工程学院，硕士生导师
2012/7 - 2019.7，华东理工大学，生物工程学院，师资博士后、讲师
三、研究方向
[1] 微藻代谢工程：微藻高价值产物的合成代谢调控及其分子机制
[2] 微藻产品工程：微藻高价值产物的高效生产工艺开发
[3] 光生物反应器：用于光合微生物的高效反应器的研制与产业化
四、代表性成果
[1] Lei Fang, Jingkui Zhang, Zhongnan Fei, Minxi Wan*(通讯作者), Astaxanthin accumulation difference between non-motile cells and akinetes of Haematococcus pluvialis was affected by pyruvate metabolism, Bioresources and Bioprocessing, 2020, 7, 5
[2] Zhen Zhang, Yingying Tan, Weiliang Wang, Wenmin Bai, Jianhua Fan, Jianke Huang, Minxi Wan*(通讯作者), Yuanguang Li*, Efficient heterotrophic cultivation of Chlamydomonas reinhardtii. Journal of applied phycology, 2019,31(3): 1545–1554
[3] Haohua Wang, Zhen Zhang, Minxi Wan*(通讯作者),, Ruixuan Wang, Jianke Huang, Kaikai Zhang, Jiacai Guo, Wenmin Bai, Yuanguang Li*, Comparative study on light attenuation models of Galdieria sulphuraria for efficient production of phycocyanin, Journal of applied phycology, 2019, 32, 165–174
[4] Minxi Wan, Zhen Zhang*, Ruixuan Wang, Wenmin Bai, Jianke Huang, Weiliang Wang, Guomin Shen, Anquan Yu, Yuanguang Li*, High-yield cultivation of Botryococcus braunii for biomass and hydrocarbons, Biomass and Bioenergy, 2019, 131, 105399
[5] Lei Fang, Jingkui Zhang, Zhongnan Fei, Minxi Wan*(通讯作者), Chlorophyll as key indicator to evaluate astaxanthin accumulation ability of Haematococcus pluvialis, Bioresources and Bioprocessing, 2019, 6, 52
[6] Weiliang Wang, Tingting Wei, Jianhua Fan, Jun Yi, Yuanguang Li*, Minxi Wan*(通讯作者), Jun Wang, Wenmin Bai. Repeated mutagenic effects of 60Co-γ irradiation coupled with high-throughput screening improves lipid accumulation in mutant strains of the microalgae Chlorella pyrenoidosaas a feedstock for bioenergy. Algal Research, 2018, 33:71-77
[7] Bin Sheng, Fei Fan, Jianke Huang, Wenmin Bai, Jun Wang, Sulan Li, WeiLi, Minxi Wan*（通讯作者）, Yuanguang Li*. Investigation on models for light distribution of Haematococcus pluvialisduring astaxanthin accumulation stage with an application case. Algal Research, 2018, 33:182-189
[8] Minxi Wan,Zhenyang Wang, Zhen Zhang, Jun Wang, Shulan Li, Anquan Yu, Yuanguang Li^*. A novel paradigm for the high-efficient production of phycocyanin from Galdieria sulphuraria. , Bioresource Technology, 2016, 218: 272-278
[9] Dongda Zhang*^#, Minxi Wan^#（并列一作）, Ehecatl A. del Rio-Chanona, Jianke Huang, Weiliang Wang, Yuanguang Li, Vassilios S. Vassiliadis*. Dynamic Modelling of Haematococcus pluvialis Photoinduction for Astaxanthin Production in both Attached and Suspended Photobioreactors, Algal Research, 2016, 13: 69-78
[10] Minxi Wan^#, Zhen Zhang^#, Jun Wang, Jianke Huang, Jianhua Fan, Anquan Yu, Weiliang Wang, Yuanguang Li*. Sequential Heterotrophy–Dilution–Photoinduction Cultivation of Haematococcus pluvialisfor efficient production of astaxanthin, Bioresource Technology, 2015, 198: 557-563
[11] Minxi Wan, Jingkui Zhang, Dongmei Hou, Jianhua Fan, Yuanguang Li *, Jianke Huang, Jun Wang, The effect of temperature on cell growth and astaxanthin accumulation of Haematococcus pluvialis during a light-dark cyclic cultivation. Bioresource Technology, 2014, 167(8):1958-1964
[12] Minxi Wan, Xuejie Jin, Jinlan Xia *, Julian N. Rosenberg, Geng Yu, Zhenyuan Nie, George A. Oyler, Michael J. Betenbaugh *. The effect of iron on growth, lipid accumulation, and gene expression profile of the freshwater microalga Chlorella sorokiniana. Applied Microbiology and Biotechnology, 2014, 98(22):9473-9481
[13] Minxi Wan^#, Dongmei Hou^#, Yuanguang Li*, Jianhua Fan, Jianke Huang, Songtao Liang, Weiliang Wang, Ronghua Pan, Jun Wang, Shulan Li. The effective photoinduction of Haematococcus pluvialisfor accumulating astaxanthin with attached cultivation. Bioresource Technology, 2014, 163(4), 26-32
[14] Minxi Wan, Junaid Faruq, Julian N. Rosenberg, Jinlan Xia, George A. Oyler, Michael J. Betenbaugh. Achieving high throughput sequencing of cDNA library utilizing an alternative protocol for the bench top next-generation sequencing system. Journal of Microbiological Methods, 2013, 92(2):122-126
[15] Minxi Wan,Runmin Wang, Jinlan Xia*, Julian N. Rosenberg, George A. Oyler, Michael J. Betenbaugh. Physiological evaluation of a new Chlorella sorokiniana isolate for its biomass production and lipid accumulation in photoautotrophic and heterotrophic cultures. Biotechnology and Bioengineering, 2012, 109(8):1958-1964
[16] Minxi Wan,Peng Liu, Jinlan Xia*, Julian N. Rosenberg, George A. Oyler, Michael J. Betenbaugh, Zhenyuan Nie, Guanzhou Qiu. The effect of mixotrophy on microalgal growth, lipid content, and expression levels of three pathway genes in Chlorella Sorokiniana. Applied Microbiology and Biotechnology, 2011, 91:835–844
[17] Minxi Wan, Julian N. Rosenberg, Junaid Faruq, Michael J. Betenbaugh, Jinlan Xia. An improved colony PCR procedure for genetic screening of Chlorellaand related microalgae. Biotechnology Letters, 2011, 33:1615–1619
[18] Minxi Wan, Yu Yang, Guanzhou Qiu*, Ailin Xu, Lin Qian, Zhiying Huang, Jinlan Xia. Acidophilic bacterial community reflecting pollution level of sulphide mine impacted by acid mine drainage. Journal of Central South University of Technology, 2009, 16: 0223?0229
[19] Minxi Wan, Yu Yang, Weimin Zeng, Jinlan Xia, Xueduan Liu, Wenqin Qin, Guanzhou Qiu^*. Succession of Bacterial Community Inhabited Acid Mine Drainage under High Fe(II) Concentration. Journal of Environmental Science and Engineering, 2010, 4(8): 46-55
[20] Feifei Han, Weiliang Wang, Yuanguang Li*, Guomin Shen, Minxi Wan*（通讯作者）, Jun Wang. Changes of biomass, lipid content and fatty acids composition under a light-dark cyclic culture of Chlorella pyrenoidosain response to different temperature. Bioresource technology, 2013, 132:182-189
五、承担项目
[1] 国家高技术研究发展计划863课题“高产虾青素的雨生红球藻‘异养-稀释-光诱导串联’培养新工艺的优化与工业化放大”（国拨经费750万，自筹经费800万）
[2] 国家重点基础研究发展计划973项目“微藻能源规模化制备的科学基础”-“基于光照方向混合及光衰减特性的光生物反应器设计与放大原理”（课题编号：2011CB200903）子课题：光生物反应器热模验证——雨生红球藻培养工艺优化与放大研究
[3] 国家自然科学基金青年科学基金项目“雨生红球藻厚壁孢子形成对虾青素合成的相关基因转录调控的影响机制”
[4] 云南省青年****课题“裸藻工业化生产技术的开发与产业化”，（省拨经费70万）
华东理工大学生物工程学院2020 年度青年交叉创新提升计划（青椒计划）项目，“异养过程中剪切力对裸藻细胞的伤害机制及其调控”
[5] 中国博士后基金，特别资助“雨生红球藻厚壁孢子形成与虾青素合成调控的关联机制”
[6] 中国博士后面上项目,一等资助“雨生红球藻异养-光诱导串联培养中细胞形态调控研究”
[7] 中央高校基本科研业务费-探索研究基金“雨生红球藻细胞形态转变规律与分子机制及其在细胞培养中的应用”
六、联系方式
上海市梅陇路130号实验18楼1101；Tel:**；
E-mail：wanminxi@ecust.edu.cn