汤丽霞
职称：教授
邮箱：lixiatang@uestc.edu.cn
所在系别：生物技术系
研究领域：蛋白质工程与生物催化



教育背景
1987年9月—1992年7月，北京师范大学，生物系，生物化学专业，本科1999年3月—2004年10月，荷兰格罗宁根大学化学系生物化学专业， 博士
工作履历
2014年7月—至今 电子科技大学，生命科学与技术学院，教授2006年10月—2014年6月， 电子科技大学，生命科学与技术学院 副教授2004年7月—2006年6月，荷兰格罗宁根大学化学系 博士后1996年9月—1999年3月，中国科学院生物物理研究所细胞室，助理研究员1992年7月—1996年9月，中国科学院生物物理研究所细胞室，实习研究员
科学研究
研究概况
多年来致力于卤醇脱卤酶等多种生物酶催化剂的基础及应用研究工作。构建了高效的卤醇脱卤酶定向进化突变体构建、筛选、鉴定平台，获得了热稳定性及催化活性同时大幅度提升的卤醇脱卤酶新变体；搭建了多种用于有机污染物检测的生物传感器系统，建立了多种有机污染物的高效生物降解通路；相关研究有效实现了卤醇脱卤酶在环境污染物降解及手性药物中间体生产中的可行应用。先后主持包括自然科学基金，科技部中克政府间合作项目等10余项科研项目，先后在国内外期刊正式发表论文60余篇，其中SCI收录的50余篇。授权发明专利3项。作为主讲教师，为本科、研究生讲授《蛋白质纯化技术》， 《蛋白质工程》，《合成生物学与IGEM》和《高级分子生物学》等课程；作为指导教师，指导本科生参加“国际遗传工程机器竞赛”（iGEM），先后取得亚太赛区银牌（2013）、总决赛银牌（2014）、总决赛金牌（2015-2019）。
代表性成果
1.IjazGul, Tadesse Fantaye Bogale, Yong Chen, Xian Yang, Ruiqin Fang, Juan Feng, Hui Gao and Lixia Tang. A paper-based whole-cell screening assay for directed evolution-driven enzyme engineering. Applied Microbiology and Biotechnology (2020). Jul;104(13):6013-6022. https://doi.org/10.1007/s00253-020-10615-x2.Ijaz Gul, Tadesse Fantaye Bogale, Jiao Deng, Le Wang, Juan Feng and Lixia Tang. A high-throughput screening assay for the directed evolution-guided discovery of halohydrin dehalogenase mutants for epoxide ring-opening reaction. Journal of Biotechnology (2020) 311: 19–24 Mar 10;311:19-24.3.Ijaz Gul, Tadesse Fantaye Bogale, Jiao Deng, Yong Chen, Ruiqin Fang, Juan Feng, Lixia Tang. Enzyme-based detection of epoxides using colorimetric assay integrated with smartphone imaging. Biotechnology and Applied Biochemistry (2020). 4.Tadesse Fantaye Bogale1, Ijaz Gul, Le Wang, Jiao Deng, Yong Chen, Maja Majeri?-Elenkov, and Lixia Tang (2020) Biodegradation of 1,2,3-trichloropropane to valuable (S)-2,3-DCP using a one-pot reaction system. Catalysts. 10, 3-135.Liangwei Yang, Hui Gao, Zhen Liu, Lixia Tang (2019). Identification of phage virion proteins by using the g-gap tripeptide composition. Letters in Organic Chemistry. 16, 332-339.6.Liangwei Yang, Hui Gao, Keyu Wu, Haotian Zhang , Changyu Li and Lixia Tang (2019). Identification of cancerlectins by using cascade linear discriminant analysis and optimal g-gap tripeptide composition. Current Bioinformatics. 2019,14, 1-10.7.Bogale Tadess Fantaye, Ijaz Gul, Lixia Tang （2019） Simulation-based optimization of bi-enzymatic system for the synthesis of enantiopure chiral compounds. the 16th ICCWAMTIP （EI收录）.8.Shao, Lifen and Gao, Hui and Liu, Zhen and Feng, Juan and Tang, Lixia and Lin, Hao. Identification of Antioxidant Proteins With Deep Learning From Sequence Information. Frontiers in Pharmacology, 2018, 9, 1036.9.Pan, Y.; Gao, H.; Lin, H.; Liu, Z.; Tang, L.; Li, S.Identification of Bacteriophage Virion Proteins Using Multinomial Na?ve Bayes with g-Gap Feature Tree. Int. J. Mol. Sci. 2018, 19, 1779.10.Liao Q, Du X, Jiang W, Tong Y, Zhao Z, Fang R, Feng J, Tang L.* （2018）Cross-linked enzyme aggregates (CLEAs) of halohydrin dehalogenase from Agrobacterium radiobacter AD1: Preparation, characterization and application as a biocatalyst. J Biotechnol. 272–273, 48–55.11.Zhiyun Wu, Wenfeng Deng, Yapei Tong, Qian Liao, Dongmin Xin, Huashun Yu, Juan Feng, and Lixia Tang*. (2017) Exploring the thermostable properties of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by a combinatorial directed evolution strategy. Applied Microbiol. Biotechnol. 101, 3201-3211.12.Juan Feng *, Qingqin Wu, Yu Zhou, Qizhou Hu, Ruiqin Fang, Lixia Tang. Interaction between flavin mononucleotide-containing Azoreductase and azo dyes. Spectroscopy letter, 2017, 49, 626-634.13.Ana Mikleu?evi?, Ines Primo?i?, Tomica Hrenar, Branka Salopek-Sondi, Lixia Tang, Maja Majeri? Elenkov. Azidolysis of epoxides catalysed by the halohydrin dehalogenase from Arthrobacter sp. AD2 and a mutant with enhanced enantioselectivity: an (S)-selective HHDH. Tetrahedron: Asymmetry (2016) 27, 930–935.14.Wang, X., Han, S., Yang, Z., Tang, L.* (2015) Improvement of the thermostability and activity of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by engineering C-Terminal amino acids. J Biotechnol., 212:92-98.（二区）15.Tang, L.*, Liu, Y., Jiang, R., Zheng, Y., Zheng, K., Zheng, H. (2015) A high-throughput adrenaline test for the exploration of the catalytic potential of halohydrin dehalogenases in epoxide-ring opening reactions. Biotechnol. Appl. Biochem. 62:451-457. 16.Wang X., Lin H., Zheng Y., Feng J., Yang Z., Tang L. *(2015) MDC-Analyzer-facilitated combinatorial strategy for improving the activity and stability of halohydrin dehalogenase from Agrobacterium radiobacter AD1.J. Biotechnol. 206:1-7. 17.Mikleu?evic′, A., Hamer?ak, Z., Salopek-Sondi, B., Tang, L. Janssen, D.B., Majeri? -Elenkov, M. (2015) Oxazolidinone synthesis through halohydrin dehalogenase catalyzed dynamic kinetic resolution. Adv. Synth. Catal. 357:1709-1714. 18.Guo, C., Chen, Y., Zheng, Y., Zhang, W., Tao, Y., Feng, J, Tang, L.*(2015) Exploring the enantioselective mechanism of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by iterative saturation mutagenesis. Appl. Environ. Microbiol. 2015 81(8):2919-26. 19.Schallmey, M., Jekel, P., Tang, L., Majeri? Elenkov, M., H?ffken, H.W., Hauer, B., Janssen, D.B. (2015) A single point mutation enhances hydroxynitrile synthesis by halohydrin dehalogenase. Enzyme Microb. Technol. 70:50-57.20.Wang, X., Zheng, Z., Zheng, H., Nie, H., Yang, Z., and Tang, L.* (2014) DC-Analyzer-facilitated combinatorial strategy for rapid directed evolution of functional enzymes with multiple mutagenesis sites. J. Biotechnol. 192:102-107。（二区）21.Tang, L., Wang, X., Ru, B., Sun, H., Huang, J., and Gao, H. (2014) MDC-Analyzer: a novel degenerate primer design tool for the construction of intelligent mutagenesis libraries with contiguous sites. BioTechniques 56:301-310. 22.Tang, L*., Zheng, K., Liu, Y., Zheng, Z., Wang, H., Song, C., Zhou, H. (2013) Exploring the potential of megaprimer PCR in conjunction with orthogonal array design for mutagenesis library. Biotechnol. Appl. Biochem. 60:190-195. 23.Bai, Y., Feng, J., Ma, H., Lin, J., Han, S., and Tang, L. (2013) Role of the CPC sequence in the antioxidant activity of GcGAST protein in E.coli. Protein J. 32: 48-57. 24.Majeri? Elenkov, M., Primo?i?, I., Hrenar, T., Smolko, A., Dokli, I., Salopek-Sondi, B., Tang, L. (2012) Catalytic activity of halohydrin dehalogenases towards spiroepoxides Org. Biomol. Chem. 10:5063-5072.（三区：IF 3.487）25.Tang, L*., Gao, H., Zhu, X., Wang, X., Zhou, M. and Jiang, R. (2012) Construction of ‘small-intelligent’ focused mutagenesis libraries using well-designed combinatorial degenerate primers. BioTechniques 52:149-158.26.Tang, L*., Zhu, X., Zheng, H., Jiang, R. and Majeri? Elenkov, M. (2012) Key residues for controlling enantioselectivity of halohydrin dehalogenase from Arthrobacter sp. AD2 revealed by structure-guided directed evolution. Appl. Environ. Microb.78:2631-2637.27.Tang, L.*., Jiang, R., Zheng, K. and Zhu, X. (2011) Enhancing the recombinant protein expression of halohydrin dehalogenase HheA in Escherichia coli by applying a codon optimization strategy. Enzyme Microb.Technol., 49: 395-401.28.Tang, L., Li, L., and Wang, X. (2010) A high-throughput colorimetric assay for screening halohydrin dehalogenase saturation mutagenesis libraries. J. Biotechnol. J. Biotechnol. 147, 164-168.
教育教学
本科生课程
《蛋白质纯化技术》、《蛋白质工程》《合成生物学与IGEM》等
研究生课程
·《高级分子生物学》
招生专业
博士：083100生物医学工程（06生物化学与分子生物学） 硕士：071000生物学（01生物化学与分子生物学）
个人/团队主页

职称 教授 邮箱 lixiatang@uestc.edu.cn
所在系别 生物技术系 研究领域 蛋白质工程与生物催化
科学研究 招生专业 博士：083100生物医学工程（06生物化学与分子生物学） 硕士：071000生物学（01生物化学与分子生物学）
联系方式 办公地址
团队联系方式 教育教学 1987年9月—1992年7月，北京师范大学，生物系，生物化学专业，本科
1999年3月—2004年10月，荷兰格罗宁根大学化学系生物化学专业， 博士
工作履历 2014年7月—至今 电子科技大学，生命科学与技术学院，教授
2006年10月—2014年6月， 电子科技大学，生命科学与技术学院 副教授
2004年7月—2006年6月，荷兰格罗宁根大学化学系 博士后
1996年9月—1999年3月，中国科学院生物物理研究所细胞室，助理研究员
1992年7月—1996年9月，中国科学院生物物理研究所细胞室，实习研究员 研究概况 多年来致力于卤醇脱卤酶等多种生物酶催化剂的基础及应用研究工作。构建了高效的卤醇脱卤酶定向进化突变体构建、筛选、鉴定平台，获得了热稳定性及催化活性同时大幅度提升的卤醇脱卤酶新变体；搭建了多种用于有机污染物检测的生物传感器系统，建立了多种有机污染物的高效生物降解通路；相关研究有效实现了卤醇脱卤酶在环境污染物降解及手性药物中间体生产中的可行应用。先后主持包括自然科学基金，科技部中克政府间合作项目等10余项科研项目，先后在国内外期刊正式发表论文60余篇，其中SCI收录的50余篇。授权发明专利3项。作为主讲教师，为本科、研究生讲授《蛋白质纯化技术》， 《蛋白质工程》，《合成生物学与IGEM》和《高级分子生物学》等课程；作为指导教师，指导本科生参加“国际遗传工程机器竞赛”（iGEM），先后取得亚太赛区银牌（2013）、总决赛银牌（2014）、总决赛金牌（2015-2019）。
代表性成果 1.IjazGul, Tadesse Fantaye Bogale, Yong Chen, Xian Yang, Ruiqin Fang, Juan Feng, Hui Gao and Lixia Tang. A paper-based whole-cell screening assay for directed evolution-driven enzyme engineering. Applied Microbiology and Biotechnology (2020). Jul;104(13):6013-6022. https://doi.org/10.1007/s00253-020-10615-x
2.Ijaz Gul, Tadesse Fantaye Bogale, Jiao Deng, Le Wang, Juan Feng and Lixia Tang. A high-throughput screening assay for the directed evolution-guided discovery of halohydrin dehalogenase mutants for epoxide ring-opening reaction. Journal of Biotechnology (2020) 311: 19–24 Mar 10;311:19-24.
3.Ijaz Gul, Tadesse Fantaye Bogale, Jiao Deng, Yong Chen, Ruiqin Fang, Juan Feng, Lixia Tang. Enzyme-based detection of epoxides using colorimetric assay integrated with smartphone imaging. Biotechnology and Applied Biochemistry (2020).
4.Tadesse Fantaye Bogale1, Ijaz Gul, Le Wang, Jiao Deng, Yong Chen, Maja Majeri?-Elenkov, and Lixia Tang (2020) Biodegradation of 1,2,3-trichloropropane to valuable (S)-2,3-DCP using a one-pot reaction system. Catalysts. 10, 3-13
5.Liangwei Yang, Hui Gao, Zhen Liu, Lixia Tang (2019). Identification of phage virion proteins by using the g-gap tripeptide composition. Letters in Organic Chemistry. 16, 332-339.
6. Liangwei Yang, Hui Gao, Keyu Wu, Haotian Zhang , Changyu Li and Lixia Tang (2019). Identification of cancerlectins by using cascade linear discriminant analysis and optimal g-gap tripeptide composition. Current Bioinformatics. 2019,14, 1-10.
7.Bogale Tadess Fantaye, Ijaz Gul, Lixia Tang （2019） Simulation-based optimization of bi-enzymatic system for the synthesis of enantiopure chiral compounds. the 16th ICCWAMTIP （EI收录）.
8.Shao, Lifen and Gao, Hui and Liu, Zhen and Feng, Juan and Tang, Lixia and Lin, Hao. Identification of Antioxidant Proteins With Deep Learning From Sequence Information. Frontiers in Pharmacology, 2018, 9, 1036.
9.Pan, Y.; Gao, H.; Lin, H.; Liu, Z.; Tang, L.; Li, S. Identification of Bacteriophage Virion Proteins Using Multinomial Na?ve Bayes with g-Gap Feature Tree. Int. J. Mol. Sci. 2018, 19, 1779.
10.Liao Q, Du X, Jiang W, Tong Y, Zhao Z, Fang R, Feng J, Tang L.* （2018）Cross-linked enzyme aggregates (CLEAs) of halohydrin dehalogenase from Agrobacterium radiobacter AD1: Preparation, characterization and application as a biocatalyst. J Biotechnol. 272–273, 48–55.
11.Zhiyun Wu, Wenfeng Deng, Yapei Tong, Qian Liao, Dongmin Xin, Huashun Yu, Juan Feng, and Lixia Tang*. (2017) Exploring the thermostable properties of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by a combinatorial directed evolution strategy. Applied Microbiol. Biotechnol. 101, 3201-3211.
12.Juan Feng *, Qingqin Wu, Yu Zhou, Qizhou Hu, Ruiqin Fang, Lixia Tang. Interaction between flavin mononucleotide-containing Azoreductase and azo dyes. Spectroscopy letter, 2017, 49, 626-634.
13.Ana Mikleu?evi?, Ines Primo?i?, Tomica Hrenar, Branka Salopek-Sondi, Lixia Tang, Maja Majeri? Elenkov. Azidolysis of epoxides catalysed by the halohydrin dehalogenase from Arthrobacter sp. AD2 and a mutant with enhanced enantioselectivity: an (S)-selective HHDH. Tetrahedron: Asymmetry (2016) 27, 930–935.
14.Wang, X., Han, S., Yang, Z., Tang, L.* (2015) Improvement of the thermostability and activity of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by engineering C-Terminal amino acids. J Biotechnol., 212:92-98.（二区）
15.Tang, L.*, Liu, Y., Jiang, R., Zheng, Y., Zheng, K., Zheng, H. (2015) A high-throughput adrenaline test for the exploration of the catalytic potential of halohydrin dehalogenases in epoxide-ring opening reactions. Biotechnol. Appl. Biochem. 62:451-457.
16.Wang X., Lin H., Zheng Y., Feng J., Yang Z., Tang L. *(2015) MDC-Analyzer-facilitated combinatorial strategy for improving the activity and stability of halohydrin dehalogenase from Agrobacterium radiobacter AD1.J. Biotechnol. 206:1-7.
17.Mikleu?evic′, A., Hamer?ak, Z., Salopek-Sondi, B., Tang, L. Janssen, D.B., Majeri? -Elenkov, M. (2015) Oxazolidinone synthesis through halohydrin dehalogenase catalyzed dynamic kinetic resolution. Adv. Synth. Catal. 357:1709-1714.
18.Guo, C., Chen, Y., Zheng, Y., Zhang, W., Tao, Y., Feng, J, Tang, L.*(2015) Exploring the enantioselective mechanism of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by iterative saturation mutagenesis. Appl. Environ. Microbiol. 2015 81(8):2919-26.
19.Schallmey, M., Jekel, P., Tang, L., Majeri? Elenkov, M., H?ffken, H.W., Hauer, B., Janssen, D.B. (2015) A single point mutation enhances hydroxynitrile synthesis by halohydrin dehalogenase. Enzyme Microb. Technol. 70:50-57.
20.Wang, X., Zheng, Z., Zheng, H., Nie, H., Yang, Z., and Tang, L.* (2014) DC-Analyzer-facilitated combinatorial strategy for rapid directed evolution of functional enzymes with multiple mutagenesis sites. J. Biotechnol. 192:102-107。（二区）
21.Tang, L., Wang, X., Ru, B., Sun, H., Huang, J., and Gao, H. (2014) MDC-Analyzer: a novel degenerate primer design tool for the construction of intelligent mutagenesis libraries with contiguous sites. BioTechniques 56:301-310.
22.Tang, L*., Zheng, K., Liu, Y., Zheng, Z., Wang, H., Song, C., Zhou, H. (2013) Exploring the potential of megaprimer PCR in conjunction with orthogonal array design for mutagenesis library. Biotechnol. Appl. Biochem. 60:190-195.
23.Bai, Y., Feng, J., Ma, H., Lin, J., Han, S., and Tang, L. (2013) Role of the CPC sequence in the antioxidant activity of GcGAST protein in E.coli. Protein J. 32: 48-57.
24.Majeri? Elenkov, M., Primo?i?, I., Hrenar, T., Smolko, A., Dokli, I., Salopek-Sondi, B., Tang, L. (2012) Catalytic activity of halohydrin dehalogenases towards spiroepoxides Org. Biomol. Chem. 10:5063-5072.（三区：IF 3.487）
25.Tang, L*., Gao, H., Zhu, X., Wang, X., Zhou, M. and Jiang, R. (2012) Construction of ‘small-intelligent’ focused mutagenesis libraries using well-designed combinatorial degenerate primers. BioTechniques 52:149-158.
26.Tang, L*., Zhu, X., Zheng, H., Jiang, R. and Majeri? Elenkov, M. (2012) Key residues for controlling enantioselectivity of halohydrin dehalogenase from Arthrobacter sp. AD2 revealed by structure-guided directed evolution. Appl. Environ. Microb.78:2631-2637.
27.Tang, L.*., Jiang, R., Zheng, K. and Zhu, X. (2011) Enhancing the recombinant protein expression of halohydrin dehalogenase HheA in Escherichia coli by applying a codon optimization strategy. Enzyme Microb.Technol., 49: 395-401.
28.Tang, L., Li, L., and Wang, X. (2010) A high-throughput colorimetric assay for screening halohydrin dehalogenase saturation mutagenesis libraries. J. Biotechnol. J. Biotechnol. 147, 164-168.
教育教学
本科生课程 《蛋白质纯化技术》、《蛋白质工程》《合成生物学与IGEM》等 研究生课程 ·《高级分子生物学》
个人团队主页 代表性学术成果
荣誉与奖励 团队主页