张纯 (Zhang Chun), 特聘副研究员
单位：四川大学 华西药学院，生物技术药物学系。
通讯地址：四川省成都市武侯区人民南路三段17号四川大学华西校区（东区）610041
E-mail: chunzhang@scu.edu.cn；zcxd118@yahoo.com

教育及工作经历
2019.02 - 至今 特聘副研究员，四川大学，华西药学院
2017.12-2019.02 抗体下游工艺开发总监，康诺亚生物医药科技（成都）有限公司
2017.12-2018.01 副研究员，硕士生导师, 生化工程国家重点实验室，中国科学院
2017.07-2017.12 副研究员，生化工程国家重点实验室，中国科学院（北京）
2014.07-2017.07 助理研究员, 生化工程国家重点实验室，中国科学院（北京）
2011.09-2014.07 四川大学，华西药学院，微生物与生化药学，理学博士

教学课程
《生物制药学》
《药用生物化学实验》
《药用分子生物学实验》

教材编写
1.《生物技术制药概论》（第4版，姚文兵主编），全国高等医药院校药学类专业第五轮规划教材，中国医药科技出版社，2019年12月，参编。
2.《药用分子生物学》（余蓉主编），化学工业出版社，2020年，参编。

研究兴趣
蛋白质/多肽药物设计、发现与筛选。

研究方向
1.偏好性激活受体信号通路的蛋白/多肽药物设计与筛选
2.药用蛋白/多肽化学修饰与长效设计
3.基于人血清白蛋白的蛋白/多肽载体递药
4.抗体/多肽偶联药物及肿瘤靶向递送
5.重组蛋白纳米颗粒胞内/胞外自组装与载药
6.生物技术药物高效下游技术研究与开发
科研项目
1.中国科协优秀中外青年交流计划，中国科协技术协会，2020/01-2020/12, 负责人。
2.国家自然科学基金委员会，面上项目，**，双靶点抗革兰阴性菌新型抗菌肽-裂解酶蛋白的理性设计与机制研究。2020/01-2023/12, 主研。
3.四川大学引进人才科研启动经费资助项目，YJ201926，2019-2022，负责人。
4.国家自然科学基金委员会，面上项目，**，病毒样蛋白颗粒疫苗胞外自组装机理及过程研究。2016/01-2019/12, 主研。
5.国家自然科学基金委员会，青年基金，**，分子辅助的蛋白质高浓度超滤膜复性的机理及应用研究。2016/01-2018/12, 主研。
6.北京市自然科学基金委员会，面上项目，**，融合蛋白中靶向肽RGD-4C定向成环策略及活性评价。2016/01-2018/12, 主研。
7.生化工程国家重点实验室开放基金，开放基金，2015KF-05，靶向双功能蛋白分子的设计及制备。2015/01-2017/12, 负责人。
8.中科院-诺和诺德专项研究经费，Novo Nordisk-CAS Research Fund，NNCAS-2014-2，Development of protein continuous refolding and purification technology。2015/01-2016/12, 主研。
9.中国医学科科学院实验动物研究所，横向课题，4920**，重组多表位人肠道病毒71型抗原蛋白制备。2014/09-2015/06, 负责人。
10.教育部博士点基金，NO.036，双功能Hv12p-rPA蛋白靶向溶栓设计及分子修饰。2013/01-2015/12, 主研。

论文发表
1.Xiangfeng Lv*, Chun Zhang*, Qingyun Shuaizhen, Rong Yu, Yongxiang Zheng, Design of integrin αvβ3 targeting self-assembled protein nanoparticles with RGD peptide, Biomedicine & Pharmacotherapy, Volume 128, 2020,
2.Chunyue Wang*, Chun Zhang*, Shuang Yin, Qi Wang, Rong Yu?, Yongdong Liu?, Zhiguo Su. Extending half-life of H-ferritin nanoparticle by fusing albumin binding domain for doxorubicin encapsulation. Biomacromolecules, 2018, 19 (3), pp 773–781.
3.Liping Liu*, Chun Zhang*, Zenglan Li, Chunyue Wang, Jingxiu Bi, Shuang Yin, Qi Wang, Rong Yu, Yong Dong Liu, and Zhiguo Su. Albumin binding domain fusing R/K-X-X-R/K sequence for enhancing tumor delivery of doxorubicin. Molecular Pharmaceutics, 2017, 14 (11), pp 3739–3749.
4.Chun Zhang*; Yu, Rong; Yongdong, Liu; Zhiguo, Su et al. Development of long-acting ciliary neurotrophic factor by site-specific conjugation with different-sized polyethylene glycols and transferrin. International Journal of Pharmaceutics. 2017; 529(1-2):275-284.
5.Yin, Shuang*; Zhang, Chun*; Li, Zenglan; Liu, Yongdong; Yu, Rong; Su, Zhiguo; Identification, characterization, and stabilization of the deamidation degradation of recombinant human tumor necrosis factor-α. Process Biochemistry. 2017; 53, pp216-223.
6.Xu Longfu*; Zhang, Chun*; Liu Yongdong, Zhiguo Su et al. Oxidized catechol-derived poly (ethylene glycol) for thiol-specific conjugation. Reactive and Functional Polymers, 117, 2017, P 97–105.
7.Zhang, Chun; Pu Jun, Yang Xiaolan, Feng Tao, Liu F, Wang D, Hu Xiaolei, Gao Ang, Liu Hongbo, Zhan Changguo, Liao Fei. Extracorporeal delivery of a therapeutic enzyme. Scientific Reports. 2016 Aug 1;6:30888. doi: 10.1038/srep30888.
8.Zhang, Chun; Liu, Yongdong; Feng, Cui; Wang, Qi; Shi, Hong; Zhao, Dawei; Yu, Rong; Su, Zhiguo, Loss of PEG chain in routine SDS-PAGE analysis of PEG-maleimide modified protein., Electrophoresis, 36(2), pp 371-374, 2015.
9.Zhang, Chun; Liu, Yongdong; Zhao, Dawei; Li, Xiunan; Yu, Rong; Su, Zhiguo, Facile purification of Escherichia coli expressed tag-free recombinant human tumor necrosis factor alpha from supernatant., Protein Expression Purification, 95, pp 195-203, 2014.
10.Zhang, Chun; Yang, Xiaolan; Gao, Ang; Hu, Xiaolei; Pu, Jun; Liu, Hongbo; Feng, Juan; Liao, Juan; Li, Yuanli; Liao, Fei, Comparison of modification of a bacterial uricase with N-hydroxysuccinimide esters of succinate and carbonate of monomethoxyl poly(ethylene glycol)., Biotechnology and Applied Biochemistry, 61(6), pp 683-690, 2014.
11.Zhang, Chun; Yang, Xiaolan; Yuan, Yong-hua; Pu, Jun; Liao, Fei, Site-specific PEGylation of therapeutic proteins via optimization of both accessible reactive amino acid residues and PEG derivatives., BioDrugs, 26(4), pp 209-215, 2012.
12.Gao, Ling*; Zhang, Chun*; Deng, Wutong; Su, Zhiguo; Yu, Rong, Construction, expression and refolding of a bifunctional fusion protein consisting of C-terminal 12-residue of hirudin-PA and reteplase., Protein Journal, 31(4), pp 328-336, 2012.
13.Zhang, Chun; Yang, Xiaolan; Feng, Juan; Yuan, Yonghua; Li, Xiang; Bu, Youquan; Xie, Yanling; Yuan, Huidong; Liao, Fei, Effects of modification of amino groups with poly(ethylene glycol) on a recombinant uricase from Bacillus fastidiosus., Biosci Biotechnol Biochem, 74(6), pp 1298-1301, 2010.
14.Fangxia Guo, Yongdong Liu, Chun Zhang, Qi Wang, Lianyan Wang, Yuhui Gao, Jingxiu Bi, Heng Wang, and Zhiguo Su, Prompt and Robust Humoral Immunity Elicited by a Conjugated Chimeric Malaria Antigen with a Truncated Flagellin. Bioconjugate Chemistry, 2018 29(3):761-770.
15.Zenglan Li, Chun Zhang, Yan Zhang, Yongdong Liu, Xiunan Li, Guanghui Ma, Jian Luo, Zhiguo Su, Prevention of aggregate formation through mechanism analysis in refolding of recombinant pertactin from Escherichia coli, Process Biochemistry, 2017, 62:80-90.
16.Wang Q, Zhang Chun, Liu LP, Li ZP, Guo FX, Li XN, Luo J, Zhao DW, Liu YD, Su ZG. High hydrostatic pressure encapsulation of doxorubicin in ferritin nanocages with enhanced efficiency. Journal of Biotechnology, 2017 20;254:34-42.
17.Wang Q, Liu YD, Zhang Chun, Guo FX, Feng C, Li XN, Shi H, Su ZG. High hydrostatic pressure enables almost 100% refolding of recombinant human ciliary neurotrophic factor from inclusion bodies at high concentration. Protein Expression and Purification, 2017, 133: 152-59.
18.Longfu Xu, Chun Zhang, Liping Liu, Yao Zhang, Qi Wang, Jian Wang, Yongdong Liu, Zhiguo Su, Purification and characterization of a long-acting ciliary neurotrophic factor via genetically fused with an albumin-binding domain, Protein Expression and Purification, Vol 139, 2017, Pages 14-20.
19.Xue L, Liu JN, Wang Q, Zhang Chun, Xu LF, Luo J, Wang J, Qin C, Liu YD, Su ZG. Purification and assembling a fused capsid protein as an enterovirus 71 vaccine candidate from inclusion bodies to pentamer-based nanoparticles. Biochemical Engineering Journal, 2017, 117: 139–146
20.Chen Y, Wang Q, Zhang Chun, Li X, Gao Q, Dong C, Liu Y, Su Z. Improving the refolding efficiency for proinsulin aspart inclusion body with optimized buffer compositions. Protein Expr Purif. 2016;122:1-7.
21.Zhao DW, Liu YD, Zhang GF, Zhang Chun, Li XN, Wang QQ, Shi H, Su ZG. Interaction of arginine with protein during refolding process probed by amide H/D exchange mass spectrometry and isothermal titration calorimetry. Biochimica et Biophysica Acta. 2015, 1854: 39–45.
22.Gao A, Yang X, Zhang Chun, Long G, Pu J, Yuan Y, Liu H, Li Y, Liao F. Facile spectrophotometric assay of molar equivalents of N-hydroxysuccinimide esters of monomethoxyl poly-(ethylene glycol) derivatives. Chem Cent J. 2012, 23;6(1):142.
23.Feng J, Chen Y, Pu J, Yang X, Zhang Chun, Zhu S, Zhao Y, Yuan Y, Yuan H, Liao F. An improved malachite green assay of phosphate: mechanism and application. Anal Biochem. 2011 Feb 1;409(1):144-9.
24.Yang X, Xie Y, Pu J, Zhao H, Liao J, Yuan Y, Zhu S, Long G, Zhang Chun, Yuan H, Chen Y, Liao F. Estimation of affinities of ligands in mixtures via magnetic recovery of target-ligand complexes and chromatographic analyses: chemometrics and an experimental model. BMC Biotechnol. 2011 May 5;11:44.
25.Feng J, Li X, Yang X, Zhang Chun, Yuan Y, Pu J, Zhao Y, Xie Y, Yuan H, Bu Y, Liao F. A new practical system for evaluating the pharmacological properties of uricase as a potential drug for hyperuricemia. Arch Pharm Res. 2010 Nov;33(11):1761-9.
26.Zhao Y, Yang X, Li X, Bu Y, Deng P, Zhang Chun, Feng J, Xie Y, Zhu S, Yuan H, Yu M, Liao F. Reversible inactivation of an intracellular uricase from Bacillus fastidiosus via dissociation of homotetramer into homodimers in solutions of low ionic strength. Biosci Biotechnol Biochem. 2009 Sep;73(9):2141-4.
27.迟胜男，李增兰，张纯^#，殷爽，冯翠，王祺，刘永东^#，苏志国；聚乙二醇20k修饰与转铁蛋白偶联睫状神经营养因子的生物活性对比研究。中国生物工程杂志； 2017 (12):59-66.
28.刘立平，张纯^#，殷爽，王祺，张耀，余蓉，刘永东^#，苏志国；白蛋白结合肽-多柔比星耦合物的设计、制备、表征及初步评价。中国生物工程杂志；2017, 37（4）68-75.
29.薛玲，张耀，张纯，王琪，刘江宁，秦川，刘永东，苏志国；亲和纯化EV71融合多表位蛋白抗原及其自组装为病毒样蛋白颗粒。中国生物工程杂志，2016，36 (7) :34-40
30.殷爽，冯翠，张纯，王祺，王健，余蓉，刘永东，苏志国；转铁蛋白-PEG-睫状神经营养因子的制备及其生物活性评价。中国生物工程杂志，2016,36（4）：43-49
31.冯翠，王祺，张纯，秦培勇，郑秀玉，王健，刘永东，苏志国；PEG定点修饰重组人睫状神经营养因子及其生物活性评价。中国生物工程杂志，2015,35（5）：15-21
32.冯翠 赵大伟 张纯 王健 秦培勇 刘永东 苏志国；一种重组人睫状神经营养因子突变体的分离纯化及结构鉴定。中国生物工程杂志，2013，10：21-27
33.甘志勇，张纯，冯娟，杨晓兰，袁拥华，廖飞，尿酸酶在用氧嗪酸建立高尿酸血症模型大鼠体内的降尿酸作用，重庆医科大学学报，2010.01.01，35（10）：1454~1456
34.Zhang, Chun; Liu Yongdong; Yu Rong，Ma Guanghui, Su Zhiguo; Instability of Thioether Bond Misleads the Observation of PEG-MAL Modified Protein in Routine SDS-PAGE Analysis，Biochemical and Molecular Engineering XVIII, 2013.06.16-20. Beijing, China. (Poster)
35.Zhang, Chun; Liu Yongdong，Feng Cui，Wang Qi，Su Zhiguo, Developing Long-Acting Ciliary Neurotrophic Factor by Conjugation with Different Size of PEGs or Transferrin, 21th Symposium Series of Young Asian Biochemical Engineers’ Community (YABEC 2015), 2015.10.14-16. Chuncheon, Korea. (Poster)
专利申请
1.ZL 3.X. 刘永东，苏志国，王祺，张纯。重组人干扰素β1b高压复性及组合层析制备方法。
2.5.9. 张纯，刘永东，苏志国。一种提高离子交换层析内毒素去除效率的方法及其用途。
3.5.9. 刘永东，张纯，苏志国。一种纳米载体蛋白及其制备方法和应用。
4.4.4. 刘永东，徐龙福，张纯，苏志国。一种长效重组睫状神经营养因子及其制备方法用途。
5.0.9. 刘永东，王祺，苏志国，张纯。一种以铁蛋白为载体的抗肿瘤药物及其制备方法。
6.1.2. 刘永东，徐龙福，苏志国，张纯，王祺。一种含有邻苯二醌类官能团的聚乙二醇修饰剂及其制备方法和用途。
7.3.0. 刘永东，张纯，苏志国。一种肿瘤靶向多肽-药物偶联衍生物、其制备方法及应用。



编辑：四川大学华西药学院