海洋毒素软骨藻酸厌氧生物转化研究

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杜苗苗,金媛,昝帅君,顾晨,张宇晴,王竞.海洋毒素软骨藻酸厌氧生物转化研究[J].,2022,62(6):551-558

海洋毒素软骨藻酸厌氧生物转化研究

Study of anaerobic biotransformation of marine toxin domoic acid

DOI：10.7511/dllgxb202206001

中文关键词:软骨藻酸厌氧生物转化降解途径微生物群落

英文关键词:domoic acid anaerobic biotransformation degradation pathway microbial community

基金项目:国家自然科学基金资助项目(42177375).

作者	单位
杜苗苗,金媛,昝帅君,顾晨,张宇晴,王竞

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中文摘要:

软骨藻酸(domoic acid，DA)是由拟菱形藻有害藻华产生的一种强烈的海洋神经毒素，严重威胁人类健康和生态安全．DA通常随着海洋雪快速沉降到底栖厌氧环境，但目前关于DA厌氧生物转化行为知之甚少．考察了海洋富集菌群BH1对DA的厌氧共代谢生物转化．结果表明，菌群BH1对1 mg/L DA厌氧生物转化率可达80%，降解符合准一级动力学反应，DA生物转化速率常数为0.099 2d-1．通过高效液相色谱串联高分辨质谱法(HPLC-HRMS/MS)鉴定DA产物，并推测了DA厌氧生物转化途径，包括生物异构化、脯氨酸环脱氢以及脱羧途径，表明厌氧生物转化是DA的重要脱毒过程．16S rRNA扩增子测序分析结果表明，DA的加入导致微生物菌群物种组成发生明显变化．优势菌属瘤胃解蛋白质菌属和脱硫弧菌属对DA具有良好的耐受性，可能是潜在的DA厌氧降解菌．研究可为全面了解DA在厌氧环境中的行为和归趋提供理论依据．

英文摘要:

Domoic acid (DA) is a powerful marine neurotoxin generated by harmful algal blooms of Pseudo nitzschiadiatoms and severely threatens human health and ecosystem safety. Commonly, DA packaged in marine snow rapidly transfers to the benthic anaerobic environment, and current knowledge on anaerobic biotransformation behavior of DA is very limited. Anaerobic cometabolic biotransformation of DA by a newly enriched marine consortium BH1 is investigated. The results show that more than 80% of DA with concentration of 1 mg/L is biotransformed with pseudo first order kinetic reaction under anaerobic conditions, and biotransformation rate constant for DA is 0.099 2d-1. Furthermore, based on DA metabolites identified by high performance liquid chromatography high resolution orbitrap mass spectrometry (HPLC-HRMS/MS), DA anaerobic biotransformation pathways are proposed, including bioisomerization, proline ring dehydrogenation and decarboxylation pathways, indicating anaerobic biotransformation is an important detoxification process for DA. 16S rRNA amplicon sequencing analysis reveals that microbial community composition has significant difference after DA addition. The genera Proteiniclasticum andDesulfovibrioare dominant during DA anaerobic biotransformation, which may jointly contribute to the elevated bacterial community resistance and biotransformation to DA. The study could provide a theoretical basis for comprehensive understanding of behavior and fate for DA in anaerobic environments.

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海洋毒素软骨藻酸厌氧生物转化研究

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