CTAB对乳腺癌细胞MCF-7和MDA-MB-231凋亡影响

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张运秋,潘悦,陶旭锋,肖桂山.CTAB对乳腺癌细胞MCF-7和MDA-MB-231凋亡影响[J].,2021,61(1):6-13

CTAB对乳腺癌细胞MCF-7和MDA-MB-231凋亡影响

Effect of CTAB on apoptosis of breast cancer cells MCF-7 and MDA-MB-231

DOI：10.7511/dllgxb202101002

中文关键词:CTAB 乳腺癌 MCF-7 MDA-MB-231 细胞凋亡机制

英文关键词:CTAB breast cancer MCF-7 MDA-MB-231 apoptosis mechanism

基金项目:国家自然科学基金资助项目(81770846).

作者	单位
张运秋,潘悦,陶旭锋,肖桂山

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中文摘要:

为探索十六烷基三甲基溴化铵(CTAB)对乳腺癌细胞凋亡的影响及其可能的作用机制，用不同浓度的CTAB作用于乳腺癌细胞系MCF-7和MDA-MB-231，经MTT实验证实，CTAB对乳腺癌细胞具有明显的杀伤作用．根据MTT实验结果确定CTAB对两种乳腺癌细胞的IC50值，通过显微镜观察根据IC50值设定浓度给药24 h对乳腺癌细胞引起的形态变化．然后根据MCF-7的IC50值设置浓度梯度进行AV-PI双染色凋亡流式细胞实验，检测不同浓度CTAB引起乳腺癌细胞凋亡的情况．最终通过Western Blot实验检测发现，乳腺癌细胞MCF-7经CTAB处理后P-bcl-2蛋白表达量降低，p53、Bax、Bim、Bad、Cleaved Caspase-3和Cleaved Caspase-9等凋亡相关蛋白的表达量升高．综上所述，CTAB可能通过激活p53信号通路诱导乳腺癌细胞线粒体内源性凋亡．

英文摘要:

To explore the effect of cetyltrimethylammonium bromide (CTAB) on breast cancer cell apoptosis and its possible mechanism, CTAB with different concentrations is applied to MCF-7 and MDA-MB-231 cell lines. MTT experiment shows that CTAB has a significant killing effect on breast cancer cells. According to the results of MTT experiment, the IC50values of CTAB on two kinds of breast cancer cells are determined. The morphological changes of breast cancer cells caused by the set concentrations according toIC50 after 24 h treatment are observed by the microscope. Then, according to the IC50 value of MCF-7, the concentration gradient is set to carry out AV-PI double staining apoptosis flow cytometry to detect the apoptosis of breast cancer cells caused by different concentrations of CTAB. Finally, Western Blot experiment shows that the expression of P-bcl-2 protein decreases, and the expression of apoptosis related proteins such as p53, Bax, Bim, Bad, Cleaved Caspase-3 and Cleaved Caspase-9 increases in breast cancer cell MCF-7 treated with CTAB. In conclusion, it is shown that CTAB may activate mitochondrial endogenous apoptosis of breast cancer cells by activating p53 signaling pathway.

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CTAB对乳腺癌细胞MCF-7和MDA-MB-231凋亡影响

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