海洋麦氏交替单胞菌共代谢降解布洛芬研究

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郭雅丽,王竞,顾晨,郭定环.海洋麦氏交替单胞菌共代谢降解布洛芬研究[J].,2021,61(1):14-21

海洋麦氏交替单胞菌共代谢降解布洛芬研究

Study of ibuprofen degradation by marineAlteromonas macleodii under co-metabolic conditions

DOI：10.7511/dllgxb202101003

中文关键词:麦氏交替单胞菌布洛芬共代谢活性氧海洋环境

英文关键词:Alteromonas macleodii ibuprofen co metabolism reactive oxygen species marine environment

基金项目:国家自然科学基金资助项目(21876018).

作者	单位
郭雅丽,王竞,顾晨,郭定环

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中文摘要:

布洛芬已在海洋环境中被检出，然而对其在海洋环境中生物降解还未见报道．为了考察海洋细菌对布洛芬的降解，了解布洛芬在海洋环境中的归趋，利用从近海沉积物中分离得到的麦氏交替单胞菌GCW，考察布洛芬共代谢降解特性、降解过程中生理特性及胞外活性氧的变化．结果表明，GCW能够共代谢降解布洛芬，3 d降解率达90%；降解过程符合准一级反应动力学，速率常数为0.061 h-1．其次，布洛芬对GCW的生长无明显影响，但对胞外聚合物、脱氢酶等生理特性均有促进作用，且能够提高NADH氧化还原酶、L-氨基酸氧化酶和铁载体(均与胞外活性氧产生相关)的活性．布洛芬降解发生在胞外，是酶和活性氧的共同作用，活性氧中羟基自由基起到最主要作用．

英文摘要:

Ibuprofen has been detected in marine environment, but its biodegradation in marine environment has not been reported yet. In order to investigate the degradation of ibuprofen by marine bacteria and understand the fate of ibuprofen in marine environment, Alteromonas macleodii GCW isolated from offshore sediments is used to study the features of ibuprofen co metabolic degradation and changes of physiological characteristics and extracellular reactive oxygen species (ROS) during degradation. According to the results,90% of ibuprofen can be degraded in 3 days. The degradation follows pseudo first order kinetics, and the degradation rate constant is 0.061 h-1. Besides,ibuprofen has no significant effect on the growth of GCW, but it can promote some physiological characteristics,such as extracellular polymeric substances and dehydrogenase activity.It can also stimulate the activity of NADH oxidoreductase, L-amino acid oxidase and siderophore, which is related to extracellular reactive oxygen species production. Ibuprofen degradation is an extracellular process that relates to both degrading enzyme and ROS, and OHplays the most important role.

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