基本信息Personal Information
副教授硕士生导师
主要任职：无
性别：男
毕业院校：大连理工大学
学位：博士
在职信息：在职
所在单位：生物工程学院
学科：生物化工
办公地点：生物工程学院547室
联系方式：**
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个人简介Personal Profile
BIOGRAPHICALName: Jun Ren Title: Associate Professor
Office Address: Room 547
Office Fax: 86-**
Office Phone: 86-**
Email Address: renjun@dlut.edu.cn
EDUCATION and TRAINING
1999-2003: Dalian University of Technology, China, B.S., Polymer
2003-2009: Dalian University of Technology, China, Ph. D., Biochemical engineering
2009-2011: Dalian University of Technology, China, Postdoctoral fellow, Biomedical engineering
Current Grant Support:
1.Fundamental Research Funds for the Central Universities (No.DUT15LAB17, 2015.01-2016.12)
2.International Science & Technology Cooperation Program of China (No. 2014DFG32590, 2014.01-2016.12).
3.Dalian High Level Talent Innovation Support Project (No.2016RQ023, 2016.10-2019.09)
4.National Major Research and Development Program (No.2016YFC**, 2016.06-2020.12)
Publications (recent years):
18. Zang, B.;Ren, J.;Li, D.;Huang, C.; Ma, H.;Peng, Q.; Ji, F.; Han, L.; Jia, L., Freezing-assisted synthesis of covalent C–C linked bivalent and bispecific nanobodies. Organic & Biomolecular Chemistry 2019, 17(2), 257-263.
17. Ren, J.; Tian, K.; Jia, L.; Han, X.; Zhao, M., Rapid covalent immobilization of proteins by phenol-based photochemical cross-linking. Bioconjugate Chemistry 2016, 27 (10), 2266-2270.
16. Gao, M.; Ren, J.; Tian, K.; Jia, L., Characterization of non-specific protein adsorption induced by triazole groups on the chromatography media using Cu (I)-catalyzed alkyne-azide cycloaddition reaction for ligand immobilization. Journal of Chromatography A 2016, 1476, 63-68.
15. Zang, B.; Ren, J.; Xu, L.; Jia, L., Direct site-specific immobilization of protein A via aldehyde-hydrazide conjugation. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 2016, 1008, 132-138.
14. Han, L.; Chu, S.; Wei, H.; Ren, J.; Xu, L.; Jia, L., Functionalized magnetic Fe3O4-beta-cyclodextran nanoparticles for efficient removal of bilirubin. Journal of Nanoscience and Nanotechnology 2016, 16 (6), 5537-5545.
13. Yang, L.; Han, L.; Ren, J.; Wei, H.; Jia, L., Coating process and stability of metal-polyphenol film. Colloids and Surfaces a-Physicochemical and Engineering Aspects 2015, 484, 197-205.
12. Wei, H.; Han, L.; Tang, Y.; Ren, J.; Zhao, Z.; Jia, L., Highly flexible heparin-modified chitosan/graphene oxide hybrid hydrogel as a super bilirubin adsorbent with excellent hemocompatibility. Journal of Materials Chemistry B 2015, 3 (8), 1646-1654.
11. Ren, J.; Han, P.; Wei, H.; Jia, L., Fouling-resistant behavior of silver nanoparticle-modified surfaces against the bioadhesion of microalgae. ACS Applied Materials & Interfaces 2014, 6 (6), 3829-3838.
10. Ren, J.; Yao, P.; Cao, Y.; Cao, J.; Zhang, L.; Wang, Y.; Jia, L., Application of cyclodextrin-based eluents in hydrophobic charge-induction chromatography: Elution of antibody at neutral pH. Journal of Chromatography A 2014, 1352, 62-68.
9. Ren, J.; Yao, P.; Chen, J.; Jia, L., Salt-independent hydrophobic displacement chromatography for antibody purification using cyclodextrin as supermolecular displacer. Journal of Chromatography A 2014, 1369, 98-104.
8. Xu, L.; Wang, C.; Chen, L.; Ren, J.; Xie, J.; Jia, L., Detection of A beta-interacting proteins via a novel A beta-adsorbents that use immobilized regular comb polymer. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 2014, 971, 94-98.
7. Ren, J.; Wang, L.; Han, X.; Cheng, J.; Lv, H.; Wang, J.; Jian, X.; Zhao, M.; Jia, L., Organic silicone sol-gel polymer as a noncovalent carrier of receptor proteins for label-free optical biosensor application. ACS Applied Materials & Interfaces 2013, 5 (2), 386-394.
6. Wei, H.; Han, L.; Ren, J.; Jia, L., Anticoagulant surface coating using composite polysaccharides with embedded heparin-releasing mesoporous silica. ACS Applied Materials & Interfaces 2013, 5 (23), 12571-12578.
5. Wei, H.; Ren, J.; Han, B.; Xu, L.; Han, L.; Jia, L., Stability of polydopamine and poly(DOPA) melanin-like films on the surface of polymer membranes under strongly acidic and alkaline conditions. Colloids and Surfaces B-Biointerfaces 2013, 110, 22-28.
4. Wang, L.; Ren, J.; Han, X.; Claes, T.; Jian, X.; Bienstman, P.; Baets, R.; Zhao, M.; Morthier, G., A label-free optical biosensor built on a low-cost polymer platform. IEEE Photonics Journal 2012, 4 (3), 920-930.
3. Wei, H.; Xu, L.; Ren, J.; Jia, L., Adsorption of bilirubin to magnetic multi-walled carbon nanotubes as a potential application in bound solute dialysis. Colloids and Surfaces a-Physicochemical and Engineering Aspects 2012, 405, 38-44.
2. Ren, J.; Bai, Y.; Hao, L.; Dong, Y.; Pi, Z.; Jia, L., Amelioration of experimental autoimmune myasthenia gravis rats by blood purification treatment using 4-mercaptoethylpyridine-based adsorbent. Journal of Biomedical Materials Research Part A 2011, 98A (4), 589-595.
1. Ren, J.; Jia, L.; Xu, L.; Lin, X.; Pi, Z.; Xie, J., Removal of autoantibodies by 4-mercaptoethylpyridine-based adsorbent. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 2009, 877 (11-12), 1200-1204.
Book chapters:
1. Jun Ren, Houliang Wei, Lingyun Jia. Adsorbents for the treatment of autoimmune diseases through hemoperfusion. Hemoperfusion: general and biospecific adsorbents, immunosorbents and leucocyte adsorbents, World Scientific, 2017, chapter 22: 629-648.
2. Jun Ren, Houliang Wei, Lingyun Jia. Blood Detoxication. Comprehensive Biotechnology (2nd edition), Elsevier, 2011, 5, 729-739.
Overview of my current research work
In general, I am very interested in understanding the mechanism of biomolecule recognition, which plays central role in a variety of life activities, and hope to exploit its potential application in different area of biotechnology and biomedical applications.
(1) Biomedical materials for blood purification treatment
One specific interest is to develop techniques that enable recognizing and separating some specific disease-related molecules directly from human blood circulation, for achieving blood purification treatment for a range of serious diseases. To this end, we have developed several strategies to design functional materials, including affinity ligand screening, camelid-derived single chain antibody or nanobody, and molecular imprinting et al. These techniques are used to synthesize selective adsorbents for removing pathogenic factors from the blood of patients suffering from autoimmune diseases, renal failure, sepsis, and even cancer. For example, we are using nanobody to develop blood purification materials to treat dialysis related amyloidosis (DRA) by selectively removing beta 2 microglobulin. Meanwhile we are also isolating nanobodies against cytokines for synthesizing selective adsorbents, which could be used to control “Cytokine Storm” by directly reducing circulating cytokines from the blood of critically-ill patients with deadly inflammation.
Currently, two of my patented blood purification adsorbents have been industrialized to develop medical appliances for treating autoimmune disease and DRA, respectively. The former has been in the stage of clinical trial in China.
(2) Biomolecular engineering for bioprocessing and biohybrid materials
Other studies focus on engineering functional proteins and developing approaches for bioseparation, biosensing, and biofunctional device design, which are mainly based on the construction of biohybrid material system. Current work is centered at the application of engineering nanobody. The development of the methodologies involves production of engineered nanobody, specific tagging, multivalued complex, site-specific modification, bioconjugation, surface modification, as well as addressing issues such as stability, efficiency and activity rate in the process of applications.
We have been developing chemical and biological methods that could enable the immobilization of nanobody or other functional proteins in a controllable and efficient manner, to facilitate the preparation of affinity chromatography media, biosensors and CTC capturing surfaces. We used photochemical crosslinking of phenol groups to immobilize proteins and prepare all-protein nanoparticles. The reactions could be completed within seconds without loss of protein activity. Nanobody-based multiprotein complexes have been designed to form multifunctional protein assemblies.
These technologies and methods are expected to be extended to other biomedical areas, such as assembly of targeted nanoparticles for drug delivery, disease detection and mechanistic studies.
教育经历Education Background
工作经历Work Experience
2003.92009.6
大连理工大学
生物化工
博士
1999.92003.7
大连理工大学
高分子专业
学士
1996.91999.7
湖北省襄樊市第五中学
无
2011.9至今
大连理工大学
讲师
2009.72011.8
大连理工大学
博士后
研究方向Research Focus
社会兼职Social Affiliations
1. Bioseparation techniques for protein purification.
2. Biomedical materials for blood purification treatments.
基本信息Personal Information
副教授硕士生导师
主要任职：无
性别：男
毕业院校：大连理工大学
学位：博士
在职信息：在职
所在单位：生物工程学院
学科：生物化工
办公地点：生物工程学院547室
联系方式：**
电子邮箱：
扫描关注
同专业硕导个人学术主页
当前位置: 中文主页 >> 科学研究
研究领域
论文成果 More>>
Peng, Qiang,Zang, Berlin,Zhao, Wei,Li, Da,Ren, Jun,Ji, Fangling,Jia, Lingyun.Efficient continuous-flow aldehyde tag conversion using immobilized formylglycine generating enzyme[J],CATALYSIS SCIENCE & TECHNOLOGY,2020,10(2):484-492
Han X.,Wang Z.,Shao Y.,Wu Z.,Ren J.,Wang J.,Teng J.,Zhao M..Temperature insensitive optical biosensor based on polymer waveguide microring[J],Guangxue Xuebao/Acta Optica Sinica,2015,35(11)
Huang, Chundong,Li, Da,Ren, Jun,Ji, Fangling,Jia, Lingyun.Generation and Application of Fluorescent Anti-Human beta 2-Microglobulin VHHs via Amino Modification[J],MOLECULES,2019,24(14)
暴晓博,任军,王玉凤,贾凌云.基于凝胶过滤色谱的β2微球蛋白标准品单体定量检测方法[J],色谱,2019,37(5):533-538
Zang, Berlin,Ren, Jun,Li, Da,Huang, Chundong,Ma, Ha,Peng, Qiang,Ji, Fangling,Han, Lulu,Jia, Lingyun.Freezing-assisted synthesis of covalent C-C inked bivalent and bispecific nanobodies[J],ORGANIC & BIOMOLECULAR CHEMISTRY,2019,17(2):257-263
Han, Xiu-You,Zhang, Hua,Bo, Shu-Hui,Morthier, Geert,Zhao, Ming-Shan,Wu, Zhen-Lin,Yang, Si-Cheng,Shen, Fang-Fang,Liang, Yu-Xin,Wang, Ling-Hua,Wang, Jin-Yan,Ren, Jun,Jia, Ling-Yun.Recent Progress of Imprinted Polymer Photonic Waveguide Devices and Applications[J],POLYMERS,2018,10(6)
专利 More>>
一种重组蛋白A亲和配基的定点固定化方法
一种光子集成阵列免标记光学生化传感检测系统
一种重组人IgE受体蛋白的制备方法及应用
一种对抗体具有广谱吸附能力的融合蛋白制备方法及应用
利用重组毕赤酵母生产蛋白A的方法
一种特异性清除甲状旁腺激素的血液净化吸附剂的制备方法
著作成果
Comprehensive Biotechnology, 2nd edition ，Volume 5: Medical Biotechnology and Healthcare/ Artificial Organs and Life Support Devices/ Blood Detoxication
Regenerative Medicine, Artificial Cells and Nanomedicine: Volume 4
科研项目
2016年大连科技之星项目(2015年称号)-用于清除肾衰中分子毒素的高效血液净化吸附剂, 省、市、自治区科技项目, 2016/10/11, 进行
利用原位点击化学构建蛋白质印迹识别界面, 2012/09/25, 完成
治疗肝衰竭肾衰竭血液净化新技术及新产品, 省、市、自治区科技项目, 2018/01/01, 进行
高性能吸附剂关键技术研发及产业化, 国家科技部 , 2016/07/28, 进行
仿生蛋白A血液净化材料及其制备方法, 企事业单位委托科技项目, 2014/12/05-2034/12/05, 进行
基本信息Personal Information
副教授硕士生导师
主要任职：无
性别：男
毕业院校：大连理工大学
学位：博士
在职信息：在职
所在单位：生物工程学院
学科：生物化工
办公地点：生物工程学院547室
联系方式：**
电子邮箱：
扫描关注
同专业硕导个人学术主页
研究领域
当前位置: 中文主页 >> 科学研究 >> 研究领域基本信息Personal Information
副教授硕士生导师
主要任职：无
性别：男
毕业院校：大连理工大学
学位：博士
在职信息：在职
所在单位：生物工程学院
学科：生物化工
办公地点：生物工程学院547室
联系方式：**
电子邮箱：
扫描关注
同专业硕导个人学术主页
论文成果
当前位置: 中文主页 >> 科学研究 >> 论文成果[1]Peng, Qiang,Zang, Berlin,Zhao, Wei,Li, Da,Ren, Jun,Ji, Fangling,Jia, Lingyun.Efficient continuous-flow aldehyde tag conversion using immobilized formylglycine generating enzyme[J],CATALYSIS SCIENCE & TECHNOLOGY,2020,10(2):484-492
[2]Han X.,Wang Z.,Shao Y.,Wu Z.,Ren J.,Wang J.,Teng J.,Zhao M..Temperature insensitive optical biosensor based on polymer waveguide microring[J],Guangxue Xuebao/Acta Optica Sinica,2015,35(11)
[3]Huang, Chundong,Li, Da,Ren, Jun,Ji, Fangling,Jia, Lingyun.Generation and Application of Fluorescent Anti-Human beta 2-Microglobulin VHHs via Amino Modification[J],MOLECULES,2019,24(14)
[4]暴晓博,任军,王玉凤,贾凌云.基于凝胶过滤色谱的β2微球蛋白标准品单体定量检测方法[J],色谱,2019,37(5):533-538
[5]Zang, Berlin,Ren, Jun,Li, Da,Huang, Chundong,Ma, Ha,Peng, Qiang,Ji, Fangling,Han, Lulu,Jia, Lingyun.Freezing-assisted synthesis of covalent C-C inked bivalent and bispecific nanobodies[J],ORGANIC & BIOMOLECULAR CHEMISTRY,2019,17(2):257-263
[6]Han, Xiu-You,Zhang, Hua,Bo, Shu-Hui,Morthier, Geert,Zhao, Ming-Shan,Wu, Zhen-Lin,Yang, Si-Cheng,Shen, Fang-Fang,Liang, Yu-Xin,Wang, Ling-Hua,Wang, Jin-Yan,Ren, Jun,Jia, Ling-Yun.Recent Progress of Imprinted Polymer Photonic Waveguide Devices and Applications[J],POLYMERS,2018,10(6)
[7]Zang, Berlin,Jia, Lingyun,Ren, Jun,Peng, Qiang.Facile preparation of covalent C-to-C linked nanobody fusions by freezing[J],ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY,2018,255
[8]贾凌云,任军.Facile preparation of covalent C-to-C linked nanobody fusions by freezing[A],2018,255:437-437
[9]臧柏林,贾凌云,任军,徐丽.冷冻法制备C端-C端连接的二价纳米抗体[A],中国化学会2017全国高分子学术论文报告会,2017,1
[10]任军,贾凌云,田凯凯.光催化交联法构建蛋白质水凝胶[A],中国化学会2017全国高分子学术论文报告会,2017,2
[11]Gao, Ming,Ren, Jun,Tian, Kaikai,Jia, Lingyun.Characterization of non-specific protein adsorption induced by triazole groups on the chromatography media using Cu (I)-catalyzed alkyne-azide cycloaddition reaction for ligand immobilization[J],JOURNAL OF CHROMATOGRAPHY A,2016,1476:63-68
[12]Ren, Jun,Tian, Kaikai,Jia, Lingyun,Han, Xiuyou,Zhao, Mingshan.Rapid Covalent Immobilization of Proteins by Phenol-Based Photochemical Cross-Linking[J],BIOCONJUGATE CHEMISTRY,2016,27(10):2266-2270
[13]贾凌云,任军,臧柏林,暴晓博.亲和分离材料的设计策略及其应用[A],2016,1-1
[14]任军,姚鹏,贾凌云.基于超分子置换洗脱模式的非盐依赖疏水色谱方法[A],2016,1-1
[15]臧柏林,任军,徐丽,贾凌云.一种重组蛋白A的定点固定化方法[A],2016,1-1
[16]Han, Lulu,Chu, Simin,Wei, Houliang,Ren, Jun,Xu, Li,Jia, Lingyun.Functionalized Magnetic Fe3O4-beta-Cyclodextran Nanoparticles for Efficient Removal of Bilirubin[J],JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2016,16(6):5537-5545
[17]贾凌云,任军,徐丽.Direct site-specific immobilization of protein A viaaldehyde-hydrazide conjugation[J],Journal of Chromatography B,2016,1008:132-138
[18]Ren, Jun,Xu, Li,Jia, Lingyun.Direct site-specific immobilization of protein A via aldehyde-hydrazide conjugation[J],JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1008:132-138
[19]Han, Xiuyou,Wu, Zhenlin,Ren, Jun,Zhao, Mingshan.Athermal optical waveguide microring biosensor with intensity interrogation[J],OPTICS COMMUNICATIONS,2015,356:41-48
[20]Han, Lulu,Ren, Jun,Jia, Lingyun.Coating process and stability of metal-polyphenol film[J],COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2015,484:197-205
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基本信息Personal Information
副教授硕士生导师
主要任职：无
性别：男
毕业院校：大连理工大学
学位：博士
在职信息：在职
所在单位：生物工程学院
学科：生物化工
办公地点：生物工程学院547室
联系方式：**
电子邮箱：
扫描关注
同专业硕导个人学术主页
专利
当前位置: 中文主页 >> 科学研究 >> 专利一种重组蛋白A亲和配基的定点固定化方法
一种光子集成阵列免标记光学生化传感检测系统
一种重组人IgE受体蛋白的制备方法及应用
一种对抗体具有广谱吸附能力的融合蛋白制备方法及应用
利用重组毕赤酵母生产蛋白A的方法
一种特异性清除甲状旁腺激素的血液净化吸附剂的制备方法
用于清除类风湿因子的血液净化材料及其制备方法
环糊精修饰磁性纳米吸附剂的制备方法及其在血液透析吸附系统中的应用
抗人β2微球蛋白的单域抗体及其制备方法和用途
一种重组蛋白A亲和配基的定点固定化方法
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基本信息Personal Information
副教授硕士生导师
主要任职：无
性别：男
毕业院校：大连理工大学
学位：博士
在职信息：在职
所在单位：生物工程学院
学科：生物化工
办公地点：生物工程学院547室
联系方式：**
电子邮箱：
扫描关注
同专业硕导个人学术主页
著作成果
当前位置: 中文主页 >> 科学研究 >> 著作成果Comprehensive Biotechnology, 2nd edition ，Volume 5: Medical Biotechnology and Healthcare/ Artificial Organs and Life Support Devices/ Blood Detoxication
Regenerative Medicine, Artificial Cells and Nanomedicine: Volume 4
共2条1/1首页上页下页尾页
基本信息Personal Information
副教授硕士生导师
主要任职：无
性别：男
毕业院校：大连理工大学
学位：博士
在职信息：在职
所在单位：生物工程学院
学科：生物化工
办公地点：生物工程学院547室
联系方式：**
电子邮箱：
扫描关注
同专业硕导个人学术主页
科研项目
当前位置: 中文主页 >> 科学研究 >> 科研项目2016年大连科技之星项目(2015年称号)-用于清除肾衰中分子毒素的高效血液净化吸附剂, 大连市青年科技之星项目, 2016/10/11, 进行
利用原位点击化学构建蛋白质印迹识别界面, 国家自然科学基金, 2012/09/25, 完成
治疗肝衰竭肾衰竭血液净化新技术及新产品, 大连市科技计划, 2018/01/01, 进行
高性能吸附剂关键技术研发及产业化, 国家重点研发计划, 2016/07/28, 进行
仿生蛋白A血液净化材料及其制备方法, 2014/12/05-2034/12/05, 进行
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