高伊函^1,,
杨宇¹,
薛娟¹,
邹堃¹,
吕丽²,
吕秀鹏¹,
邓晓琴¹,
于湛^1,,
1. 大连医科大学附属第一医院 放疗科，辽宁 大连 116011
2. 大连医科大学附属第二医院 病理科，辽宁 大连 116027

基金项目: 辽宁省自然科学基金指导计划（2019-ZD-0633）


详细信息 作者简介: 高伊函（1996-），女，硕士研究生。E-mail：937696431@qq.com








通讯作者: 于 湛，主治医师。E-mail：yz1529@hotmail.com 中图分类号: R739


摘要:目的探讨Septin7蛋白在胶质瘤中的表达情况及其临床意义。方法通过免疫组织化学方法检测188例胶质瘤患者石蜡标本中Septin7蛋白表达情况，分析与患者临床病理参数（WHO分级、性别、年龄、KPS、术中切除情况、肿瘤大小、肿瘤部位、肿瘤局限于单叶或多叶）的关系，并结合是否行放疗，是否口服替莫唑胺及生存情况进行预后分析。结果随着胶质瘤WHO分级的升高，Septin7蛋白表达降低（P＜0.05）。女性胶质瘤患者中Septin7蛋白表达水平较男性患者高（P＜0.05），Septin7表达在不同年龄、KPS评分、切除情况、肿瘤部位、肿瘤是否局限于单叶情况间比较，差异均无统计学意义（P＞0.05）。不同Septin7蛋白表达组之间生存时间差异有统计学意义（P＜0.05）。单因素Cox分析显示，性别（P=0.003）、年龄（P=0.002）、WHO分级（P=0.001）、是否行放疗（P=0.005）及Septin7蛋白表达程度（P=0.020）是胶质瘤预后的影响因素。将单因素Cox分析中具备统计学意义的因素纳入多因素Cox逐步回归分析，结果提示性别（HR=0.618，95%CI 0.429～0.889，P=0.009）、年龄（HR=1.707，95%CI 1.099～2.651，P=0.017）、WHO分级（HR=2.683，95%CI 1.452～4.959，P=0.002）及是否行放疗（HR=2.090，95%CI 1.499～2.914，P＜0.001）为预后的独立影响因素。结论Septin7蛋白在女性胶质瘤患者中表达水平更高。随着脑胶质瘤WHO分级提高，Septin7蛋白表达程度降低，与胶质瘤患者预后不良相关，但暂无法作为独立预后因子。
关键词: 胶质瘤/
Septin7蛋白/
免疫组化


Abstract:ObjectiveTo investigate the expression of Septin7 protein in glioma and its clinical significance.MethodsImmunohistochemical method was used to detect the expression of Septin7 protein in paraffin samples of 188 patients with glioma. Prognostic analysis was carried out based on the clinical and pathological parameters (WHO grade, gender, age, KPS, intraoperative resection, tumor size, tumor site, tumor confined to single or multiple lobes), radiotherapy, oral temozolomide and survival of patients.ResultsWith the increase of WHO grade of glioma, the expression of Septin7 protein decreased (P＜0.05). The expression level of Septin7 protein in female patients with glioma was higher than that in male patients (P＜0.05). There were no significant differences of the expression of Septin7 in different ages, KPS scores, resection conditions, tumor sites, and whether the tumor was confined to a single lobe (P＞0.05). The patients' survival was significant different among different groups of protein expression (P＜0.05). Univariate Cox analysis revealed that gender (P=0.003), age (P=0.002), WHO grade (P=0.001), radiotherapy (P=0.005) and the expression of Septin7 protein (P=0.020) were the prognostic factors of glioma. The factors with statistical significance in the univariate Cox analysis were further included in multivariate Cox stepwise regression analysis. The results indicated that gender (HR=0.618, 95% CI 0.429-0.889, P=0.009), age (HR=1.707, 95% CI 1.099-2.651, P=0.017), WHO classification (HR=2.683, 95% CI 1.452-4.959, P=0.002) and radiotherapy (HR=2.090, 95% CI 1.499-2.914, P＜0.001) were independent prognostic factors.ConclusionThe expression level of Septin7 protein is higher in female patients with glioma. With the increase of WHO grade of glioma, the expression of Septin7 protein decreases, which is related to the poor prognosis of glioma patients, although it cannot be used as an independent prognostic factor currently.
Keywords:glioma/
Septin7 protein/
immunohistochemistry

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