吴英杰
吴英杰，男，理学博士, 教授，博士生导师。大连医科大学重大疾病基因工程模式动物研究所所长,科技部基因工程模式动物国际联合研究中心主任,美国西奈山医学院兼职副教授。
辽宁省攀登学者，教育部创新团队骨干成员；国家重大人才工程 “创新人才推进计划”领军人才和创新团队评议专家，国家国际科技合作专项项目及合作基地评审专家；国际华人骨研学会教育委员会委员。
E-mail：yingjiewu@dmu.edu.cn，yyjjwu@yahoo.com, yingjie.wu@mssm.edu
联系地址：大连医科大学 重大疾病基因工程模式动物研究所
通讯地址：辽宁省大连市旅顺南路西段9号，116044
个人经历
工作经历
2013.03——至今 大连医科大学重大疾病基因工程模式动物研究所 教授，所长
2012.12—至今 美国纽约大学口腔医学院基础科学与颅面生物学系 研究员， 兼职教授
2011.09—至今 美国西奈山医学院内分泌、糖尿病及骨疾病系 兼职副教授
2005.09—2011.08 美国西奈山医学院内分泌、糖尿病及骨疾病系 研究副教授
2002.02—2005.03 美国国立健康卫生研究院心肺血液研究所 资深研究员
1999.01—2002.02 美国宾夕法尼亚大学生物系 研究助理
1989.07—1992.02 辽宁师范大学食品生化所 讲师
教育经历
1995.12—1998.12 美国宾夕法尼亚大学生物系，博士后。
导师：Anthony Cashmore 博士（美国科学院院士）
1992.02—1995.07中国农业大学分子生物学及生物化学专业，博士。
导师：阎隆飞教授（中国科学院院士）
1986.09—1989.07沈阳农业大学生物化学专业，硕士。
导师：曹淡君教授
1982.09—1986.07东北师范大学生物学专业，学士
研究领域
1、人类重大疾病基因工程模式动物的研发、应用与产业化。主要利用改进的高通量Cre/LoxP系统、TALEN以及CRISPR/Cas9介导的基因编辑技术建立组织特异性基因敲除动物模型，研究相关基因在不同时间、组织或细胞中在正常及病理状态下的功能，为研究疾病分子机制及治疗奠定理论基础，促进转化医学的发展。
2、利用基因工程模式动物对GH-IGF1轴信号通路中基因如生长激素(Growth hormone)、类胰岛素生长因子(IGF1)和胰岛素(Insulin)等在生长发育及病理状态下如肥胖、糖尿病、骨疾病、癌症及老年病中的作用。
3、癌症（如非小细胞肺癌，前列腺癌）和慢病（如脂肪肝）临床早期诊断Marker 和试剂盒。
4、利用基因敲除动物模型研究中药活性成分的分子作用机制，尤其是内分泌疾病和肿瘤。
研究成果
研究成果及贡献
1、首次利用转基因小鼠与基因敲除小鼠相结合战略建立了只有内分泌形式的IGF1小鼠模型，从而解决了三十年来悬而未决的内分泌IGF1与旁分泌、自分泌IGF1的功能之分。相关研究论文已在Endocrinology连续发表。
2、建立生长激素受体基因Flox小鼠并在此基础上建立了系列组织特异性基因敲除小鼠，如肝、肌肉、脂肪、胰岛、骨、神经组织生长激素受体基因敲除小鼠模型，进而研究生长激素受体在生长发育、衰老及病理状态下的功能，开展生长激素受体功能的系统研究。为相关疾病治疗及药物研发奠定理论基础。相关研究论文已在The Journal of Clinical Investigation 和Diabetes发表。
3、建立的小鼠基因编码框优化的FLPo重组酶工具鼠，100％高效性删除FRT两侧修饰的选择标记基因NEO。已被美国、英国、德国和加拿大及等二十多个实验室索取并使用。相关研究论文于2009年底发表在PLoS ONE，下载或阅读11320余次。
4、建立类胰岛素生长因子IGF1 点突变（R3）和缺失（DES）Knock-In小鼠，相关研究论文于2011年底发表在Disease Models & Mechanisms。
5、建立新的Laron综合征的小鼠动物模型——即利用肝脏特异表达IGF1转基因小鼠与生长激素受体基因缺失（在人类上既为Laron综合征患者）杂交产生。
6、利用先进的基因编辑技术构建中医病症结合动物模型，把模式动物引入到中医药的研究中，围绕IGF-1轴开展中医治疗糖尿病、中医肾本质的研究，拓展了中西医结合研究的思路，开创了中西医结研究的新领域。
7、搭建基础研究与临床研究的桥梁，建立我国第一个专门以模式动物研究为主要任务的国际联合研究中心——科技部国家级国际合作基地——“基因工程模式动物国际联合研究中心”。将之作为国际化“造动物—养动物—用动物”的科技合作基地。致力于人类重大疾病基因工程模式动物的研发、应用与产业化。
8、利用特有的基因修饰小鼠模型研究中草药天然产物及新型高分子生物材料对前列腺癌和肝癌以及代谢疾病如肥胖，脂肪肝，糖尿病的发病机理及治疗。
奖励/荣誉
1、2013 年入选辽宁省第五批高等学校攀登学者
2、2012年第六次国际生长激素和胰岛素样生长因子研究大会（德国），GroPep奖
3、2008 年第四次国际生长激素和胰岛素样生长因子研究大会（意大利），旅行奖
4、2015年入选辽宁省第二批“十百千工程”“千人”层次
学术及社会兼职
1、国家重大人才工程“创新人才推进计划”领军人才和创新团队评议专家
2、国家国际科技合作专项项目评审专家
3、国家国际科技合作基地评审专家
4、中国博士后科学基金评审专家
5、国际华人骨研学会教育委员会委员
6、美国内分泌学会会员
7、国际IGF 研究学会会员
8、美国骨岩研究学会会员
9、中美医药协会会员
10、国际华人骨研学会会员
指导和培养博士、硕士研究生情况
毕业博士研究生2人；硕士研究生6人，在读博士研究生4人，硕士研究生9人。
学术期刊审稿人
Diabetes, Endocrinology, Molecular Endocrinology, Journal of Endocrinology and Journal of Molecular Endocrinology
科研项目
1、国家自然科学基金面上项目：组织特异性敲除GHR基因在β细胞功能中的作用机制研究，项目编号81471000，78万元，2015.01-2018.12，主持
2、国家国际科技合作专项项目：利用新的高效基因打靶技术建立人类重大疾病动物模型，项目编号2014DFA32120，456万，2014.04-2017.03，主持
3、辽宁省科技计划项目：基因工程实验动物新品系及人类重大疾病动物模型研发，项目编号201402893，20万元，2014.04-2017.03，主持
4、辽宁省省自然科学基金优秀人才培育项目：建立高效靶向基因修饰系统CRISPR/Cas9研究糖尿病相关基因的功能，项目编号2014023042，5万元， 2014.07-2016.06，主持
5、高等学校博士学科点专项科研基金课题：利用IGF1R基因突变2型糖尿病模型小鼠研究石斛滋阴作用及机制，项目编号20132105110002，12万，2014.01-2016.12，主持
6、国家人力资源与社会保障部 2013年留学人员科技活动择优资助项目：建立高效TALEN与CRE-LoxP相结合系统研究糖尿病相关基因的功能， 8万元，主持
7、2013年大连市第四批海创工程项目：利用植物生物反应器生产长效胰岛素，项目编号22012100106，100万， 2013.12 -2016.12，主持
8、国家国际科技合作专项项目：利用分子农业生产药用蛋白和生物活性物质，项目编号2012DFA30450，440万元， 2012.05-2015.04，美方负责人（中方合作单位山东省高新技术研究中心）
9、国家国际科技合作项目：可吸收骨折内固定器件和骨组织工程修复支架的联合研发，项目编号2010DFB50890， 1434万元，2010.01-2011.12，美方负责人（中方合作单位中国科学院长春应用化学研究）
10、美国NIH 课题（NIH/DDHS ）：IGF and IGF Receptor Function in Mammary Development，项目编号5R01DK060612-09，2007.09-2013.07，Co-Investigator
申报专利
吴英杰，景作磊等。 一种细胞DNA损伤修复的抑制剂 (专利申请号等待中)
发表的学术论文或专著
1、代表性研究论文（选自62篇论文）(*Co-first authors, # Correspondence author)
[1] Zhang Y, Zou X, Qian W, Weng X, Zhang L, Zhang L, Wang S, Cao X, Ma L, Wei G#, Wu Y#, Hou Z#. Enhanced PAPSS2/VCAN sulfation axis is essential for Snail-mediated breast cancer cellmigration and metastasis. Cell Death Differ, 2018, [Epub ahead of print].
[2] Tao Yu, Chunxin Wang, Jian Yang, Yuqi Guo, Yingjie Wu# and Xin Li#. Metformin inhibits SUV39H1-mediated migration of prostate cancer cells. Oncogenesis， (2017) e324; doi:10.1038.
[3] Yuqi Guo, Chengzhi Xie, Xiyan Li, Jian Yang, Yu Tao, Tianqing Zhang, Michael Snyder, Yingjie Wu and Xin Li#. Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis. Nature Communications， 2017 May 31;8:15621.
[4] Jinhui Yu, Yong Ma, Jie Sun, Liyuan Ran, Youwei Li, Ning Wang, Tao Yu, Wenting Gao, Wenbin Jia, Rujiao Jiang, Meihua Guo, Yuping Bi# and Yingjie Wu# Microalgal oil from Schizochytrium sp. prevents HFD-induced abdominal fat accumulation in mice. Journal of the American College of Nutrition， 2017 May 26:1-10.
[5] Yiwen Qin, Yuanzhen Peng, Wei Zhao, Jianping Pan, Hanna Ksiezak-Reding, Christopher Cardozo, Yingjie Wu, Paola Divieti Pajevic, Lynda F. Bonewald, William A Bauman and Weiping Qin. Myostatin Inhibits Osteoblastic Differentiation by Suppressing Osteocyte-derived Exosomal microRNA-218: A Novel Mechanism in Muscle-Bone Communication. J Biol Chem， 2017;292(26):11021-11033. PMID: 28465350
[6] Yuqi Guo, Tao Yu, Jian Yang, Tianqing Zhang, Yang Zhou, Fan He, Zoya Kurago, David Myssiorek, Yingjie Wu, Peng Lee, Xin Li Metformin inhibits salivary adenocarcinoma growth through cell cycle arrest and apoptosis. Am J Cancer Res 2015，5 (12):3600-3611
[7] Weiping Qin, Xiaodong Li, Yuanzhen Peng, Lauren M Harlow, Yinshi Ren, Yingjie Wu, Jiliang Li, Yiwen Qin, Jie Sun, Tom Brown, Jian Q. Feng, Hua Zhu Ke, William A Bauman, Christopher C Cardozo. Sclerostin Antibody Preserves the Morphology and Structure of Osteocytes and Blocks the Severe Skeletal Deterioration after Motor-Complete Spinal Cord Injury in Rats. Journal of Bone and Mineral Research. 2015, 30(11):1994–2004
[8] Zhenwei Gong*, Oran Kennedy*, Hui Sun*, Yingjie Wu*, Garry A Williams, Laura Klein, Luis Cardoso, Ronald W. Matheny Jr, Gene B. Hubbard, Yuji Ikeno, Roger P. Farrar, Mitchell B Schaffler, Martin L Adamo, Radhika H Muzumdar1 and Shoshana Yakar, Reductions in serum IGF-1 during aging impair health span. Aging Cell, 2014, 13, 408–418.
[9] Kennedy OD, Sun H, Yingjie Wu, Courtland HW, Williams GA, Cardoso L, Basta-Pljakic J, Schaffler MB, Yakar S. Skeletal response of male mice to anabolic hormone therapy in the absence of the Igfals gene. Endocrinology, 2014, 155(3):987-999.
[10] Yingjie Wu, Hui Sun, JelenaBasta-Pljakic, Luis Cardoso, Oran D Kennedy, Hector Jasper, HoracioDomené, Liliana Karabatas, Clara Guida, Mitchell B Schaffler, Clifford J Rosen, Shoshana Yakar, Serum IGF-1 is insufficient to restore skeletal size in the total absence of the growth hormone receptor. Journal of Bone and Mineral Research, 2013, 28(7),1575-1586.
[11] Vijayakumar A, Buffin NJ, Gallagher EJ, Blank J, Yingjie Wu, Yakar S, Leroith D. Deletion of Growth Hormone Receptors in Post-Natal Skeletal Muscle of Male Mice Does Not Alter Muscle Mass and Response to Pathological Injury. Endocrinology，2013 , 154(10):3776-3783.
[12] Tunde Akinyeke,Satoko Matsumura, Xinying Wang, Yingjie Wu, Eric D.Schalfer, AnjanaSaxena, Wenbo Yan, SusanK.Logan and Xin Li. Metformin targets c-MYC oncogene to prevent prostate cancer. Carcinogenesis, 2013, 134(12): 2823-2832.
[13] Courtland HW, Kennedy OD, Yingjie Wu, Gao Y, Sun H, Schaffler MB, Yakar S. Low levels of plasma IGF-1 inhibit intracortical bone remodeling during aging. Age, 2013,，35(5), 1691-1703.
[14] ArchanaVijayakumar*, Yingjie Wu*, ChengyuLiu,Hui Sun, Shoshana Yakar and Derek LeRoith, Targeted loss of GHR signaling in mouse skeletal muscle protects from high-fat diet-induced metabolic deterioration. Diabetes, 2012, 61(1):94-103.
[15] ArchanaVijayakumar, Yingjie Wu, Buffin NJ, Li X, Sun H, Gordon RE, Shoshana Yakar, Derek LeRoith. Skeletal muscle growth hormone receptor signaling regulates Basal, but not fasting-induced, lipid oxidation. PLoS ONE, 2012，7(9):e44777.
[16] Yingjie Wu, ChengyuLiu,Hui Sun, ArchanaVijayakumar, PejmanRaeisiGiglou, Joshua Oppenheimer, Shoshana Yakar and Derek LeRoith.Growth hormone receptor regulates β cell hyperplasia and glucose-stimulated insulin secretion in obese mice. The Journal of Clinical Investigation, 2011, 121(6):2422–2426.
[17] Sebastien Elis*, Yingjie Wu*, Hayden-William Courtland, Dara Cannata, Hui Sun, Mordechay Beth-On, Hector Jasper, HoracioDomené, Liliana Karabatas, Clara Guida, JelenaBasta-Pljakic, Luis Cardoso, Clifford J Rosen, and Shoshana Yakar. Unbound (bioavailable) IGF1 enhances somatic growth. Disease Models & Mechanisms, 2011 ,4(5):649-658.
[18] Sebastien Elis, Yingjie Wu, Hayden-William Courtland, Hui Sun, Clifford J Rosen, Adamo ML and Shoshana Yakar. Increased serum IGF-1 levels protect the musculoskeletal system but are associated with elevated oxidative stress markers and increased mortality independent of tissue igf1 gene expression. Aging Cell, 2011, 27.(10) 1474-9726.
[19] Hayden-William Courtland, Sebastien Elis, Yingjie Wu, Hui Sun, Clifford J Rosen, Karl J. Jepsen and Shoshana Yakar. Serum IGF-1 Affects Skeletal Acquisition in a Temporal and Compartment-Specific Manner. PLoS ONE, 2011, 6(3): e14762.
[20] Hayden-William Courtland, Hui Sun, Mordechay Beth-On,Yingjie Wu, Sebastien Elis, Clifford J Rosen and Shoshana Yakar. Growth hormone mediates pubertal skeletal development independent of hepatic IGF-1 production. Journal of Bone and Mineral Research, 2011, 26(4): 761–768.
[21] Guo-Ying Huang, Li-Jian Xie, Kaari L. Linask, Chen Zhang, Xiao-Qing Zhao, Yi Yang, Guo-Min Zhou, Yingjie Wu, Lucrecia Marquez-Rosado, Doff B. McElhinney, Elizabeth Goldmuntz, Chengyu Liu, Paul D. Lampe, Bishwanath Chatterjee, and Cecilia W. Lo. Evaluating the role of connexin43 in congenital heart disease: Screening for mutations in patients with outflow tract anomalies and the analysis of knock-in mouse models. J Cardiovasc Dis Res, 2011, 2(4): 206–212.
[22] Yingjie Wu, Pnina Brodt, Hui Sun, Wilson Mejia, RuslanNovosyadlyy, Nomeli Nunez, Xiaoli Chen, Arnulfo Mendoza, Sung-Hyeok Hong, Chand Khanna and Shoshana Yakar.Insulin-like growth factor-I regulates the liver microenvironment in obese mice and promotes liver metastasis. Cancer Research，2010, 70(1):57-67.
[23] Dara Cannata, Danielle Lann, Yingjie Wu, Sebastien Elis, Hui Sun, Shoshana Yakar, Deborah A. Lazzarino, Teresa L. Wood and Derek LeRoith, Elevated Circulating IGF-1 Promotes Mammary Gland Development and Proliferation. Endocrinology, 2010, 151(12): 5751-5761.
[24] Sebastien Elis, Hayden-William Courtland, Yingjie Wu, J. Christopher Fritton, HuiSun, Clifford J Rosen and Shoshana Yakar. Elevated serum IGF-1 levels synergize PTH action on the skeleton only when the tissue IGF-1 axis is intact. Journal of Bone and Mineral Research, 2010, 25(9):2051-8.
[25] Sebastien Elis, Hayden-William Courtland, Yingjie Wu, Clifford J Rosen, Hui Sun, Karl J Jepsen, Robert J Majeska and Shoshana Yakar. Elevated serum levels of IGF-1 are sufficient to establish normal body size and skeletal properties even in the absence of tissue IGF-1. Journal of Bone and Mineral Research, 2010, 25(6):1257-1266.
[26] J. ChristopherFritton, Yuki Kawashima, Wilson Mejia, Hayden-Williams Courtland,Sebastien Elis, Hui Sun, Yinjgie Wu, Clifford J. Rosen, David Clemmons and Shoshana Yakar. The insulin-like growth factor-1 (IGF-1) binding protein acid-labile subunit (ALS) alters mesenchymal stromal cell fate. The Journal of Biological Chemistry, 2010, 285(7):4709-4714.
[27] J Christopher Fritton, Kelly B Emerton, Hui Sun, Yuki Kawashima, Wilson Mejia, Yingjie Wu, Clifford J Rosen, David Panus, Mary Bouxsein, Robert J Majeska,Mitchell B Schaffler and Shoshana Yakar. Growth Hormone Protects Against Ovariectomy-Induced Bone Loss in States of Low Circulating IGF-1. Journal of Bone and Mineral Research, 2010, 25(2):235-246.
[28] Shoshana Yakar, Clifford J. Rosen, Mary L. Bouxsein, Hui Sun, Wilson Mejia, Yuki Kawashima, Yingjie Wu, Kelly Emerton, Valerie Williams, Karl Jepsen, Mitchell B. Schaffler, Robert J. Majeska, Oksana Gavrilova, Mariana Gutierrez, David Hwang, Patricia Pennisi, Jan Frystyk, Yves Boisclair, John Pintar, Héctor Jasper, HoracioDomene, Pinchas Cohen, David Clemmons and Derek LeRoith. Serum complexes of insulin-like growth factor-1 modulate skeletal integrity and carbohydrate metabolism. The FASEB Journal, 2009, 23(3):709-719.
[29] Yingjie Wu, Hui Sun, Shoshana Yakar, Derek LeRoith. Elevated levels of IGF-1 in serum rescue the severe growth retardation of IGF-1 null mice. Endocrinology, 2009,150(9):4395-4403.
[30] Yingjie Wu, Chunxin Wang, Hui Sun, Derek LeRoith and Shoshana Yakar. High-Efficient FLPo Deleter Mice in C57BL/6J Background. PLoS ONE, 2009, 4(11): e8054.
[31] Xiao-Ning Zhang*, Yingjie Wu*, John Tobias, Brian Brunk, Gerald Deitzer, Dongmei Liu. HFR1 is crucial for transcriptome regulation in cryptochrome 1-mediated early response to blue light in Arabidopsis thaliana. PLoS ONE, 2008,3(10): e3563, (*Co-first authors).
[32] Yuka Toyoshima, Christopher Monson, CunmingDuan, Yingjie Wu, Chuan Gao, Shoshana Yakar, Kirsten C. Sadlerand Derek LeRoith. The role of insulin receptor signaling in zebrafish embryogenesis. Endocrinology,2008,149(12):5996-6005.
[33] Jinhui Zhang, Yingjie Wu, Yuanyuan Zhang, Derek LeRoith, David A. Bernlohr and Xiaoli Chen. The Role of Lipocalin 2 in the Regulation of Inflammation in Adipocytes and Macrophages. Molecular Endocrinology, 2008, 22(6):1416-26.
[34] Charlie Xiang, Yingjie Wu. Li Ma, L. Ding &I. Lisinski, M. J. Brownstein,Samuel W Cushman and Xiaoli Chen. Characterisation of insulin-resistant phenotype of cultured rat primary adipose cells. Diabetologia, 2007, 50(5):1070-1079.
[35] Qing Yu, Y. Shen, B. Chatterjee, B. H. Siegfried, L. Leatherbury, J. Rosenthal, J. F. Lucas, A. Wessels, C. F. Spurney, Yingjie Wu, M. L. Kirby, K. Svenson and C. W. Lo. ENU induced mutations causing congenital cardiovascular anomalies. Development, 2004,131(24):6211-6223.
[36] Hongquan Yang, Yingjie Wu, Dongmei Liu, Ruhang Tang and Anthony R. Cashmore. The C-terminal of Arabidopsis cryptochrome mediate a constitutive light response. Cell, 2000,103: 815-827.
[37] Anthony R. Cashmore, Jarillo, J. A., Yingjie Wu and Dongmei Liu. Cryptochromes: Blue Light Receptors for Plant and Animals. Science, 1999, 284: 760-765.
2、综述论文
[1] Shuang Wang, Jin Wu, Ning Wang, Li Zeng and Yingjie Wu, The Role of Growth Hormone Receptor on β Cell Function. Growth Hormone & IGF Research, 2017, 36:30-35. (# Correspondence author)
[2] ArchanaVijayakumar, RuslanNovosyadlyy, Yingjie Wu, Shoshana Yakar and Derek LeRoith. Biological effects of growth hormone on carbohydrate and lipid metabolism. Growth Hormone & IGF Research, 2010, 20(1):1-7.
[3] Yingjie Wu and Lungfei Yen. Motor protein in microtubule. Progressing Biochemistry, 1996, 1(1): 1-8.
3、专著
[1] GENOME EDITING and ENGINEERING: From Talens, ZFNs and CRISPRs to Molecular Surgery. edited by Dr. Krishnarao Appasani, Cambridge University Press 2017 (One Chapter: Genome editing with desired mutations (knock-in) with CRISPR in model organisms, Chunxin Wang and Yingjie Wu).
[2] Biochemistry and Nutrition of Food. The Scientific Press, Beijing, China, 1994. Three Chapters.
十二、学术会议演讲
[1] Dong Yu, Xiaoxin Wang, Jin Wu, Rujiao Jiang and Yingjie Wu Growth Hormone Receptor Play a Key Role in the Pathogenesis of Nonalcoholic Fatty Liver Disease, the Cold Spring Harbor Asia conference on Lipid Metabolism and Metabolic Disorders. Suzhou, China, April 10-14, 2017.
[2] 中美创新对话---基因工程模式动物与医学研究国际论坛，2014年11月22-24日，中国大连. β-cell specific disruption of the growth hormone receptor impair β cell insulin secretion.（本所为会议主办方）
[3] The BIT’s 3rd Annual World Congress of Endometabolism and Diabetes, on May 20-22, 2013. Xi’an, China. Serum IGF-1 is insufficient to restore body size in the absence of growth hormone receptor.
[4] The 6th International Congress for the GRS and IGF Society, on October 17-20, 2012. Munich, Germany. Serum Insulin-like growth factor (IGF)-I is insufficient to restore linear growth in the absence of growth hormone receptor.
[5] The Endocrine Society's 93rd Annual Meeting & Expo. on June 4-8, 2011.Boston, Massachusettes. Serum IGF-I is insufficient to restore body size of the GHR null mouse.
[6] The Fifth International Congress of the GRS and the IGF Society onOct.3-7, 2010. New York, New York. β-cell specific disruption of the growth hormone receptor impair β-cell insulin secretion.
[7] The Endocrine Society's 92nd Annual Meeting & Expo on June 19-22, 2010. San Diego, California. Targeted ablation of growth hormone receptors in the skeletal muscle improves metabolism and protects against diet-induced obesity in mice.
[8] The Endocrine Society's 91st Annual Meeting on June 10-13, 2009. Washington, DC,. Local IGF-1 is essential for the development of female reproductive system and normal estrus cycle.
[9] The Fourth International Congress for the GRS and IGF Society, on September 16-20, 2008. Genova, Italy. Liver-derived circulating IGF-I is sufficient to restore linear growth in an IGF-I null mouse.
[10] The Endocrine Society's 90th Annual Meeting on June 15-18, 2008. San Francisco, California. Liver-derived, circulating (endocrine) Insulin-like Growth factor-1, is sufficient to support normal post-natal growth.
十三、学术会议墙报
[1] Xiaoshuang Wang, Liyuan Ran, Xiangdong Liu, Shuang Wang and Yingjie Wu
Effects of Adipose Tissue-specific GHR Knockout on the Lipid Metabolism in Mice the Cold Spring Harbor Asia conference on Lipid Metabolism and Metabolic Disorders. Suzhou, China, April 10-14, 2017.
[2] Archana Vijayakumar, Yingjie Wu, Hui Sun, Chengyu Liu, N. Haddad, Shoshana Yakar, and Derek LeRoith Growth Hormone Signaling Facilitates Muscle - Fat Crosstalk to Mediate Insulin Resistance. The Endocrine Society's 93st Annual Meeting & Expo, Boston June 4-8, 2011.
[3] Yingjie Wu, Hui Sun, ArchanaVijayakumar, Sheng Yao, Chengyu Liu, Shoshana Yakar, and Derek LeRoith. b-Cell Specific Disruption Of The Growth Hormone Receptor Does Not Affect b-Cell Mass But May Affect Its Function. The Endocrine Society's 92st Annual Meeting & Expo, San Diego, California, June 19-22, 2010.
[4] DELann, Yingjie Wu, H Sun, S Yakar, D LeRoith. Elevated Levels of Circulating IGF-I Can Rescue Mammary Gland Development of IGF-I Null Mice. The Endocrine Society's 91st Annual Meeting, Washington, DC, June 10-13, 2009.
[5] Y Kawashima, CJ Fritton, S Elis, HW Courtland, H Sun, Yingjie Wu, W Mejia, CJ Rosen, DR Clemmons, S Yakar. Reduced IGF-1 Complex Formation Leads to mesenchymal stromal cell fateThe Endocrine Society's 91st Annual Meeting, Washington, DC, June 10-13, 2009.
[6] HW Courtland, S Elis, Yingjie Wu, H Sun, CJ Rosen, KJ Jepsen, S Yakar. Depletion of Serum IGF-1 after Peak Bone Acquisition Does Not Affect Skeletal Architecture in the Adult Mouse. The Endocrine Society's 91st Annual Meeting, Washington, DC, June 10-13, 2009.
[7] S Elis, HW Courtland, Yingjie Wu, H Sun, V Williams, K Jepsen, S Yakar. Sex-Specific Effects of Endocrine IGF-1 on Skeletal Morphology and Peak Bone Acquisition.The Endocrine Society's 91st Annual Meeting, Washington, DC, June 10-13, 2009.
[8] JC Fritton, Y Kawashima, H Sun, W Mejia, Yingjie Wu, CJ Rosen, D Panus, ML Bouxsein, RJ Majeska, MB Schaffler, S Yakar Growth Hormone Protects Against Ovariectomy-Induced Bone Loss in States of Low Circulating Insulin-Like Growth Factor-I. The Endocrine Society's 91st Annual Meeting, Washington, DC, June 10-13, 2009.
[9] Shoshana Yakar, Ernesto Canalis, Wilson Mejia, Hui sun, Yingjie Wu, Yuki Kawashima Phil Nasser, Valerie Williams and Karl Jepsen. Serum IGF-1 is a developmental determinant of bone size. The 30th Annual Meeting of the American Society for Bone and Mineral Research, Montréal, Quebéc, Canada, September 12 -16, 2008
[10] J Christopher Fritton, Kelly Emerton, Hui Sun, Yuki Kawashima, Alexander Sinofsky, Wilson Mejia, Yingjie Wu, Mitchell Schaffler, Mary Bouxsein, Clifford J Rosen and Shoshana Yakar. Low serum IGF-I alters the cortical bone response to estrogen deficiency. The 30th Annual Meeting of the American Society for Bone and Mineral Research, Montréal, Quebéc, Canada, September 12 -16, 2008
[11] Yingjie Wu, Sebastien Elis, Hui sun, Yuki Kawashima and Shoshana Yakar. Endocrine IGF-1 maintains linear growth in the total absence of tissue IGF-1. The 30th Annual Meeting of the American Society for Bone and Mineral Research, Montréal, Quebéc, Canada, September 12 -16, 2008
[12] RadoslavSavic, Kenjiro Inagaki, Yingjie Wu, Hui Sun, RuslanNovosyadlyy, Yuki Kawashima, shoshanayakar and derekleroith: Oxidative stress in MKR mouse model of type-2 diabetes, The Endocrine Society's 90th Annual Meeting, San Francisco, June 15-18, 2008.
[13] Jinhui Zhang, Yingjie Wu, Yuanyuan Zhang, Derek LeRoith, David A. Bernlohr, and Xiaoli Chen: The role of lipocalin 2 in the regulation of inflammation in adipocytes and macrophages. Workshop on the Establishment, Maintenance, & Turnover of Fat Depots, National Institutes of Health, Bethesda, MD May 21-22, 2008.
[14] RuslanNovosyadlyy, Shoshana Yakar, Yingjie Wu, Kenjiro Inagaki, RadoslavSavic, Hui Sun, Derek LeRoith: Endoplasmic Reticulum Stress-Induced Apoptosis in Human Breast Cancer Cells. The Endocrine Society’s 88th Annual Meeting, Boston June24-27, 2006
[15] Kenjiro Inagaki, Anatoly Tiulpakov, Shoshana Yakar, Yingjie Wu, RuslanNovosyadlyy, RadoslavSavic, Hui Sun, Derek LeRoith: Functional Abnormalities of Cells with the R481Q Point Mutation in the Insulin-Like Growth Factor-I Receptor Gene. The Endocrine Society’s 88th Annual Meeting, Boston June24-27, 2006
[16] Yingjie Wu, E.Doe, C. Liu, and C. W. Lo:High throughput recovery of mouse Cx47 mutations with a genotype base screen of embryonic stem cells using DHPLC and Cel I endonuclease:The44th Annual Meeting of The American Society for Cell Biology (ASCB), Washington, December 4-8, 2004
[17] Yingjie Wu, C. Rosenow and C. Lo: High-Throughput Analysis of mutations and sequence variations in mouse connexin genes. The International gap junction conference, St. John’s Colleage, University of Cambridge, United Kingdom, August, 2003.
[18] Yingjie Wu, C. Rosenow and C. Lo: High-Throughput discovery of mouse connexin mutations using resequencing DNA chip. Symposium on phenotyping mouse cardiovascular function and development, National Institutes of Health, Bethesda, Maryland. October, 2002.
孙杰
孙杰，女，1980年12月出生，辽宁大连人，硕士，讲师。
个人经历
2007.07-2014.05任职于大连医科大学中西医结合学院（研究院）
2014.06至今任职于大连医科大学重大疾病基因工程模式动物研究所。
研究方向及荣誉
从事中西医结合内分泌疾病基础研究，掌握分子生物学技术、组织形态学技术、实验动物技术等。主要研究领域为：中药对胰岛素抵抗动物模型的作用及其机制研究。主持省教育教学课题1项，参与国家级课题4项。在中文核心期刊上发表学术论文6篇。
冉丽媛
冉丽媛，女，1986年10月出生，博士，讲师。
个人经历
2005年9月至2009年6月就读于西北师范大学生命科学学院；
2009年9月至2014年6月于山东大学微生物技术国家重点实验室硕博连读，获得理学博士学位；
2014年10月进入大连医科大学重大疾病基因工程模式动物研究所担任讲师
研究方向及荣誉
主要从事基因工程模式动物构建及肥胖等代谢疾病相关基因功能研究。参与国家级省部级课题多项，参与发表SCI论文7篇，累计影响因子32.7，其中以第一作者身份在Journal of Biological Chemistry, Molecular Microbiology发表SCI论文2篇，影响因子9.626，作为第二参与人授权国家发明专利两项。
姜如娇
姜如娇，女，1984年6月出生，辽宁大连人，硕士。
个人经历
2002.09-2006.07于大连理工大学环境与生命学院攻读生物工程学士学位。
2006.09-2009.07于大连理工大学环境与生命学院攻读生物化学与分子生物学硕士学位。
2009.11-2010.12于上海长岛生物技术有限公司担任研发专员。
2011.11-至今担任大连医科大学助理实验师。
研究方向及荣誉
从事基因工程模式动物和代谢疾病基础研究多年。主要研究领域为利用基因工程模式动物进行非酒精性脂肪肝发病机理和治疗研究。参与国家科技合作项目、国家自然基金面上项目等多项国家级和省部级科研项目。
刘波
刘波，女，1983年2月出生，山西大同人，博士，讲师。
个人经历
2009.09-2012.07于大连医科大学口腔医学院口腔医学专业，攻读硕士研究生。
期间，主要参与移植骨髓间充质干细胞再生修复唇腭裂组织的研究。
2012.09-2015.07继续于大医科大学病理学与病理生理学专业攻读博士学位。
研究方向及荣誉
主要研究方向为颌面部发育畸形和组织病理学研究。博士在读期间，利用维甲酸诱导腭裂小鼠模型观察胚鼠舌的发育过程中正常及异常的组织形态学及超微结构变化，探讨舌发育异常的分子调控机制，该部分结果已于2014年发表于Sci Rep.（IF=5.078）。利用microRNA芯片技术对维甲酸诱导舌发育异常胚鼠舌组织进行差异microRNA的筛选并进行进一步的生物信息学分析，对差异microRNA靶基因进行信号通路和功能的富集性分析，深入探讨microRNA在维甲酸诱导的舌发育异常中的作用机制。此外，利用三维体系在体外条件下进行小鼠胚胎腭器官培养的研究成果，已申请国家发明专利并获批受理（申请号：201310657627.X）。本人曾多次参与国内、国际学术会议并发表多篇会议论文。
郭美华
郭美华，女，1987年12月出生，辽宁抚顺人，硕士，助理实验员。
个人经历
2007.10—2011.07大连医科大学生物技术系，取得学士学位；
2011.09—2014.07大连医科大学生物技术系，取得硕士学位。
2014.10于大连医科大学重大疾病基因工程模式动物研究所工作至今。
研究方向及荣誉
主要研究方向为：利用动物模型进行骨代谢相关基因的研究。参与国家级省级课题多项，以第一作者发表SCI论文1篇，IF:3.116，题目： Inhibition of cell proliferation, migration and invasion by a glioma-targeted fusion protein combining the p53 C terminus and MDM2-binding domain.
李有为
李有为，男，1989年10月出生，黑龙江省兰西县人，硕士学位，助理实验师。
个人经历
2012.09—2015.06在山东农业大学动物科技学院胚胎工程实验室完成硕士研究生课题。期间参与束缚应激对后代小鼠行为影响及印记基因表达差异的研究，山羊精液液态保存体系和保存期间主要葡萄糖代谢通路研究，与凋亡通路相关的Fas/FasL蛋白在不同物种成熟精子的定位表达，山羊精子发生过程中各时期细胞Fas/FasL的表达及与凋亡的关系研究，凋亡相关Fas/FasL与生精细胞凋亡关系的研究。硕士期间发表中文核心期刊论文1篇，SCI期刊论文1篇。
2015年6月毕业于东北农业大学生命科学学院，发育生物学专业。
2016.01—2016.04于日本东京理科大学生命医科学研究院岩仓教授实验室学习小鼠胚胎操作等相关技术。
2015.07至今，就职于大连医科大学重大疾病基因工程模式动物研究所，助理实验师。
研究方向及荣誉
主要从事实验室转基因小鼠的生产工作。熟练掌握山羊精子的采集和稀释保存方法，熟练掌握小鼠卵母细胞获取，体外成熟，IVF，胚胎快速冷冻保存和复苏，雄鼠输精管结扎，小鼠胚胎移植和受精卵显微注射等相关技术。
王宁
王宁，男，1990年3月出生，山东省临沂人，硕士学位，助理实验员。
个人经历
2007.10—2012.07 滨州医学院中西医结合临床学院，取得学士学位；
2012.07-2013.06 工作于蓬莱市解放军第405医院普外科
2013.09—2016.07大连医科大学中西医结合基础学院，取得硕士学位。
2015.10—2016.01于日本东京理科大学生命医科学研究院岩仓教授实验室学习小鼠胚胎操作等相关技术。
2016.10于大连医科大学重大疾病基因工程模式动物研究所工作至今。
研究方向及荣誉
主要研究方向：主要从事利用CRISPR/Cas9技术进行转基因小鼠的构建工作。熟练掌握打靶载体构建、小鼠胚胎移植和受精卵显微注射等相关技术。
