摘要： 目的 研究子痫前期胎盘组织中神经型Wiskott-Alrdich综合征蛋白（N-WASP）的表达情况及其临床意义。方法 选取2020年7月至2021年12月于中国医科大学附属盛京医院分娩的65例孕产妇作为研究对象，并将其分为早发型子痫前期组（15例）、早发对照组（15例）、晚发型子痫前期组（15例）、晚发对照组（20例）。采用实时定量PCR （RT-qPCR）检测各组胎盘组织中N-WASP mRNA的相对表达量，采用Western blotting和免疫组织化学法检测各组胎盘组织中N-WASP蛋白的表达情况及定位情况。结果 RT-qPCR、Western blotting结果显示，早发型子痫前期组胎盘组织N-WASP mRNA和蛋白表达水平显著低于早发对照组（0.50±0.19 vs.0.93±0.73，0.35±0.17 vs.0.72±0.21），晚发型子痫前期组胎盘组织中N-WASP mRNA和蛋白表达水平显著低于晚发对照组（0.83±0.34 vs.1.15±0.34，0.39±0.16 vs.0.76±0.20），差异均有统计学意义（均P < 0.05）。N-WASP mRNA在胎盘组织中的表达与早发型及晚发型子痫前期的发生均呈负相关（r=-0.37，P = 0.042；r=-0.39，P = 0.019）。免疫组织化学检测显示N-WASP蛋白定位于合体滋养细胞、细胞滋养细胞、绒毛间质细胞和血管内皮细胞的胞质中。结论 N-WASP低表达可能与子痫前期的发生密切相关。

子痫前期胎盘组织中神经型Wiskott-Alrdich综合征蛋白的表达及其意义

张硕¹, 葛云鹏¹, 王婷婷¹, 沈鸿飞¹, 李佳钋¹, 宋贵玉¹, 乔宠^1,2,3,4

1. 中国医科大学附属盛京医院妇产科, 沈阳 110039;
2. 国家卫生健康委员会生殖健康与遗传医学重点实验室, 沈阳 110039;
3. 辽宁省母胎医学重点实验室, 沈阳 110039;
4. 中国医科大学出生队列研究中心, 沈阳 110039

收稿日期:2023-03-22出版日期:2024-02-28发布日期:2024-01-12
通讯作者:乔宠E-mail:qiaochong2002@163.com
作者简介:张硕(1988-),女,主治医师,博士研究生.
基金资助:国家自然科学基金（81370735，81771610）；百千万人才工程领军人才项目（XLYC2005008）；辽宁省民生科技计划联合计划（2021JH2/10300123）；沈阳市科技计划（20-205-4-004）


关键词: 神经型Wiskott-Alrdich综合征蛋白, 子痫前期, 滋养细胞
Abstract: Objective To study the expression and clinical significance of neural Wiskott-Alrdich syndrome protein (N-WASP) in placentas with preeclampsia. Methods This study included a total of 65 pregnant women:15 in the early-onset preeclampsia group, 15 in the early-onset control group, 15 in the late-onset preeclampsia group, and 20 in the late-onset control group. Real-time fluorescence quantitative PCR (RT-qPCR) was used to detect the relative expression of N-WASP mRNA in placental tissues. Western blotting and immunohistochemistry were used to detect the expression and position of N-WASP protein in placental tissues from each group. Results RT-qPCR revealed significantly lower N-WASP mRNA expression levels in the placental tissue of the early-onset preeclampsia group compared to those in the early-onset control group (0.50±0.19 vs. 0.93±0.73, P < 0.05). The N-WASP mRNA expression levels in late-onset preeclampsia placenta were significantly lower than those in the late-onset control group (0.83±0.34 vs. 1.15±0.34, P < 0.05). Western blotting revealed significantly lower N-WASP protein expression in the placental tissue of early-onset preeclampsia compared to that in the early-onset control group (0.35±0.17 vs. 0.72±0.21, P < 0.05). The N-WASP protein expression in late-onset preeclampsia placenta was significantly lower than that in the late-onset control group (0.39±0.16 vs. 0.76±0.20, P < 0.05). The N-WASP mRNA expression in the placenta negatively correlated with the occurrence of early-onset (r=-0.37, P=0.042) and late-onset preeclampsia (r=-0.39, P=0.019). Immunohistochemistry revealed that N-WASP protein was localized in the cytoplasm of syncytiotrophoblasts, cytotrophoblasts, villous stromal cells, and vascular endothelial cells. Conclusion The low expression of N-WASP may be closely associated with preeclampsia.
Key words: neural Wiskott-Alrdich syndrome protein, preeclampsia, trophoblast
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