摘要： 目的 探讨紫铆因诱导SIRT1激活对大鼠坐骨神经损伤的影响及其可能的机制。方法 将30只大鼠随机分为假手术组、坐骨神经损伤组和紫铆因组，每组10只。分别于建立大鼠坐骨神经损伤模型手术当天、术后第7天和第14天检测各组大鼠BBB运动评分和坐骨神经功能指数；术后第14天取材，通过HE染色观察各组大鼠坐骨神经病理学改变，通过TUNEL实验检测各组大鼠坐骨神经细胞凋亡水平，通过Western blotting检测各组大鼠坐骨神经BDNF、MBP、GAP-43、SIRT1、FOXO1、Keap1和NF-κB蛋白表达水平。结果 与坐骨神经损伤组大鼠相比，紫铆因组坐骨神经损伤大鼠BBB运动评分和坐骨神经功能指数增加，坐骨神经病理损伤改善，坐骨神经细胞凋亡水平降低，坐骨神经BDNF、MBP、GAP-43和SIRT1蛋白表达水平增高，FOXO1、Keap1和NF-κB蛋白表达水平降低。结论 紫铆因可通过上调坐骨神经损伤大鼠SIRT1表达抑制FOXO1/NF-κB信号通路激活，继而改善大鼠坐骨神经病理损伤。

紫铆因通过激活SIRT1介导FOXO1/NF-κB信号通路改善坐骨神经损伤

车敏, 张辉

沈阳医学院附属中心医院手外一科, 沈阳 110024

收稿日期:2023-05-08出版日期:2024-02-28发布日期:2024-01-12
通讯作者:张辉E-mail:59526543@qq.com
作者简介:车敏(1982-),男,副教授,博士.
基金资助:国家自然科学基金（8197080581）；辽宁省自然科学基金（2020-MS-146）；辽宁省高校基本科研项目（LJKMZ20221781）


关键词: 紫铆因, 坐骨神经损伤, 沉默信息调节因子2相关酶1, FOXO1/NF-κB信号通路
Abstract: Objective This study aimed to explore the effect and possible mechanism of SIRT1 activation induced by butein on sciatic nerve injury in rats. Methods A total of 30 rats were randomly divided into a sham operation group, a sciatic nerve injury group, and a butein group, with 10 rats in each group. BBB motor scores and sciatic nerve function index were detected on the modeling surgery day, the 7th day after surgery, and the 14th day after surgery. The pathological changes of the sciatic nerve in each group were observed by HE staining. The apoptosis of sciatic nerve cells in each group was detected by TdT-mediated dUTP nick end labeling (TUNEL). The expression of BDNF, MBP, GAP-43, SIRT1, FOXO1, Keap1, and NF-κB in the sciatic nerve was detected by Western blotting. Results Butein improved the pathological injury of the sciatic nerve, reduced the apoptosis of sciatic nerve cells, increased BDNF, MBP, GAP-43, and SIRT1 expression, and decreased FOXO1, Keap1, and NF-κB expression in the sciatic nerve. Conclusion Butein can inhibit FOXO1/NF-κB signaling pathway activation by up-regulating SIRT1 expression in rats with sciatic nerve injury and then improve the sciatic nerve pathological injury in rats.
Key words: butein, sciatic nerve injury, SIRT1, FOXO1/NF-κB signaling pathway
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