摘要： 目的 探讨双酚A（BPA）及其替代物双酚AF（BPAF）、双酚B（BPB）、双酚S（BPS）是否可引起肝细胞脂质蓄积。方法 BPA、BPAF、BPB、BPS处理HL-7702细胞24 h后，通过油红O染色观察细胞内脂肪蓄积情况；GPO-PAP酶法测定细胞内甘油三酯含量；实时PCR测定细胞内甘油三酯合成相关基因LIPIN2和DGAT2 mRNA的表达水平。结果 油红O染色显示，BPA、BPAF、BPB、BPS组细胞内均可观察到大小不等的红色脂滴。甘油三酯含量测定发现，1、50 μmol/L BPA组，10、50 μmol/L BPAF组，1、10、50 μmol/L BPB组，50 μmol/L BPS组细胞内甘油三酯含量明显增加；实时PCR结果显示，10 μmol/L BPAF组，10 μmol/L BPB组及1、10 μmol/L BPS组细胞内LIPIN2 mRNA表达水平明显升高，50 μmol/L BPA组、10 μmol/L BPAF组及10、50 μmol/L BPB组和1 μmol/L BPS组细胞内DGAT2 mRNA表达水平明显升高。结论 BPA、BPAF、BPB、BPS可通过上调DGAT2 mRNA表达促进细胞内甘油三酯合成增加，BPAF、BPB、BPS还可通过上调LIPIN2 mRNA表达促进细胞内甘油二酯合成，导致HL-7702细胞内脂肪蓄积。

双酚A及其3种替代物对肝细胞甘油三酯合成的影响

陈阳¹, 高文婷¹, 李肖¹, 姬晓彤¹, 白剑英^1,2

1. 山西医科大学公共卫生学院环境卫生学教研室, 太原 030001;
2. 煤炭环境致病与防治教育部重点实验室, 太原 030001

收稿日期:2023-04-21出版日期:2023-12-30发布日期:2023-12-12
通讯作者:白剑英E-mail:jybai66@aliyun.com
作者简介:陈阳(1998-),男,硕士研究生
基金资助:山西省自然科学基金（202203021211231）


关键词: 双酚A, 双酚A类似物, 甘油三酯合成
Abstract: Objective To investigate whether BPA and its substitutes BPAF, BPB, and BPS can induce lipid accumulation in hepatocytes.Methods HL-7702 cells were treated with BPA, BPAF, BPB, or BPS for 24 h. Lipid droplet accumulation in cells was observed via oil red O staining, and triglyceride content was determined through the GPO-PAP chemical-enzymatic method. In addition, qRT-PCR was used to detect the expression of triglyceride synthesis-related genes.Results Red lipid droplets of different sizes could be observed in the BPA, BPAF, BPB, and BPS groups. The intracellular triglyceride content increased significantly under 1 and 50 μmol/L BPA, 10 and 50 μmol/L BPAF, 1, 10, and 50 μmol/L BPB, and 50 μmol/L BPS. LIPIN2 mRNA expression level increased significantly under 10 μmol/L BPAF, 10 μmol/L BPB, and 1 and 10 μmol/L BPS. DGAT2 mRNA expression increased significantly under 50 μmol/L BPA, 10 μmol/L BPAF, 10 and 50 μmol/L BPB, and 1 μmol/L BPS.Conclusion BPAF, BPB, and BPS can increase intracellular triglyceride synthesis by upregulating LIPIN2 and DGAT2 mRNA expression, leading to intracellular fat accumulation and increased triglyceride content in HL-7702 cells.
Key words: bisphenol A, bisphenol A analogues, synthesis of triglyceride
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