摘要： 目的 基于代谢组学探讨定喘汤治疗呼吸道合胞病毒（RSV）感染毛细支气管炎的疗效。方法 选取2016年9月至2017年2月于我院接受治疗的30例RSV感染的毛细支气管炎患儿为研究对象（毛细组），另选同期于我院体检的38例健康儿童为对照组。采用LC-MS/MS行代谢组学检测，对比2组儿童的代谢产物，毛细组患儿均给予定喘汤治疗，通过代谢组学检测探讨定喘汤发挥疗效的机制。结果 与对照组相比，毛细组代谢改变差异明显的代谢产物为3个溶血磷脂酰胆碱（LPC） （LPC 18：2 sn-1、LPC 18：0 sn-1和LPC 18：0 sn-2），苯丙氨酸（Phe），3个磷脂酰胆碱（PC） （PC 34：3、PC 34：2、PC 34：1）；毛细组接受定喘汤治疗前后代谢改变差异明显的代谢产物主要为LPC 18：0 sn-1、LPC 18：0 sn-2、LPC 16：0 sn-1，Phe，PC 34：2，鞘磷脂（SM） 34：1以及去甲-戊二酰胺；治疗后代谢产物变化最明显的为LPC 18：0 sn-1和LPC 18：0 sn-2。结论 利用代谢组学可区分RSV感染致毛细支气管炎患儿在接受定喘汤治疗前后的不同代谢模式，其中脂代谢产物差异最明显，可认为是定喘汤发挥治疗作用的重要机制。

定喘汤治疗呼吸道合胞病毒感染的毛细支气管炎的代谢组学研究

朱紫微, 崔振泽, 单玉霞, 迟磊

大连市妇女儿童医疗中心(集团) 小儿呼吸科, 辽宁 大连 116000

收稿日期:2023-06-01出版日期:2023-09-30发布日期:2023-09-02
通讯作者:迟磊E-mail:chilei00070@163.com
作者简介:朱紫微(1988-),女,主治医师,硕士.
基金资助:国家自然科学基金（81473726）


关键词: 呼吸道合胞病毒, 毛细支气管炎, 代谢组学, 定喘汤
Abstract: Objective Based on metabonomics, this study analyzes the therapeutic effect of Dingchuan decoction on bronchiolitis caused by respiratory syncytial virus (RSV). Methods Thirty children with RSV bronchiolitis treated in our hospital between September, 2016, and February, 2017, were selected as research subjects, and 38 healthy children examined in our hospital during the same period were selected as the control group. Metabonomic analysis was performed using LC-MS/MS to compare the metabolites between the two groups. All children in the capillary group were treated with Dingchuan decoction, and the therapeutic effect of the Dingchuan decoction was investigated using metabonomics to elucidate its mechanism of action. Results Metabonomic analysis revealed significant metabolic differences in children with RSV bronchiolitis compared to healthy children, primarily involving three lysophosphatidylcholine (LPC) mole-cules:LPC 18:2 sn-1, LPC 18:0 sn-1, and LPC 18:0 sn-2. Additionally, phenylalanine (Phe), and three phosphatidylcholines (PC 34:3, PC 34:2, and PC 34:1) showed significant metabolic changes. In children with RSV bronchiolitis treated with the Dingchuan decoction, notable metabolic changes were primarily observed in three LPC molecules:LPC18:0 sn-1, LPC 18:0 sn-2, and LPC 16:0 sn-1. Additionally, Phe, PC 34:2, SM34:1, and norvalerolac (Delta-valerolactam) displayed significant differences in metabolic profiles before and after treatment. Notably, the most pronounced changes were observed in LPC 18:0 sn-1 and LPC 18:0 sn-2. Conclusion Metabolomics analysis could be used to distinguish the different metabolic patterns in children with RSV bronchiolitis before and after treatment with Dingchuan decoction. Moreover, the most significant difference in lipid metabolites could be considered as an important mechanism of the Dingchuan decoction exerting its therapeutic effect.
Key words: respiratory syncytial virus, bronchiolitis, metabonomics, dingchuan decoction
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