摘要： 目的 探讨红景天苷对链脲佐菌素（STZ）诱导的糖尿病大鼠视网膜病变（DR）的保护作用。方法 将32只雄性SD大鼠随机分为对照组、糖尿病组及红景天苷低、高剂量（50、200 mg/kg）组。腹腔注射STZ建立糖尿病大鼠模型。模型诱导成功后，灌胃给予红景天苷12周。苏木素和伊红染色观察视网膜组织的病理学改变；荧光定量PCR、免疫印迹及酶联免疫吸附试验检测视网膜内相关mRNA和蛋白的表达情况。结果 与对照组相比，糖尿病组视网膜神经节细胞层排列紊乱、视网膜神经节细胞（RGC）密度明显降低（P<0.05）；视网膜环氧合酶-2 （COX-2）、血管内皮生长因子（VEGF） mRNA及蛋白表达水平显著升高（P<0.01），前列腺素E2 （PGE2）分泌水平显著升高（P<0.01）。与糖尿病组相比，红景天苷低剂量组COX-2和VEGF mRNA表达以及PGE2分泌水平显著下降（P<0.05）；红景天苷高剂量组RGC层排列情况有所改善，RGC密度明显升高（P<0.05），COX-2和VEGF mRNA及蛋白表达（P<0.05）和PGE2分泌水平（P<0.01）显著降低。结论 红景天苷能改善糖尿病大鼠视网膜组织病理改变，其机制可能与抑制COX-2/PGE2/VEGF通路相关。

红景天苷通过COX-2/PGE2/VEGF通路对糖尿病大鼠视网膜病变的影响

张庆龙¹, 赵宁², 李芷鉴³

1. 锦州医科大学 附属第一医院心内科, 辽宁 锦州 121001;
2. 锦州医科大学 基础医学院生物化学与分子生物学教研室, 辽宁 锦州 121001;
3. 锦州医科大学 口腔医学院, 辽宁 锦州 121001

收稿日期:2023-01-12出版日期:2023-08-30发布日期:2023-08-07
通讯作者:赵宁E-mail:zhaoningjzykdx@163.com
作者简介:张庆龙(1988-),男,主治医师,硕士.
基金资助:辽宁省教育厅科学研究经费项目（LJKQZ20222423）


关键词: 红景天苷, 糖尿病视网膜病变, COX-2/PGE2/VEGF信号通路
Abstract: Objective This study aimed to document the protective effects of salidroside on streptozotocin (STZ) -induced retinopathy in diabetic rats. Methods Male Sprague-Dawley rats were randomly divided into a control group,diabetic group,and low- and high-dose salidroside groups (50 and 200 mg/kg,respectively). STZ was intraperitoneally injected to establish diabetic rat models at a dose of 65 mg/kg. After successful induction of the model,salidroside was given by gavage for 12 weeks. Hematoxylin-Eosin staining was used to observe pathological changes in retinal tissues and detect the density of retinal ganglion cells (RGCs). Fluorogenic quantitative polymerase chain reaction,Western blotting,and ELISA were used to detect the expression of mRNA and proteins in the retina. Results Compared to the control group,the arrangement of RGC layers was disordered,their density was significantly reduced (P < 0.05),and the mRNA and protein expression levels of COX-2 and VEGF (P < 0.01) and secretion levels of PGE2 (P < 0.01) were significantly increased. Compared to the diabetic group,the mRNA expression of COX-2 and VEGF as well as the PGE2 secretion levels were improved in the low-dose salidroside group (P < 0.05). In the high-dose salidroside group,RGC layer arrangement was improved,the density of RGCs significantly increased (P < 0.05),and the mRNA and protein expression levels of COX-2 and VEGF (P < 0.05) and secretion levels of PGE2 (P < 0.01) were significantly decreased compared to the diabetic group. Conclusion Salidroside can relieve retinal pathologic changes in diabetic rats. The mechanism may be related to the downregulation of the COX-2/PGE2/VEGF pathway.
Key words: salidroside, diabetic retinopathy, COX-2/PGE2/VEGF signaling pathway
PDF全文下载地址:

https://journal.cmu.edu.cn/CN/article/downloadArticleFile.do?attachType=PDF&id=3268