摘要： 目的 探讨系统性红斑狼疮（SLE）患者外周血中T细胞α7烟碱型乙酰胆碱受体（α7nAChR）mRNA的表达及其临床意义。方法 选取2019年7月至12月于中国医科大学附属第一医院风湿免疫科住院的16例女性SLE患者作为SLE组，以性别、年龄相匹配的16例女性健康志愿者作为正常对照组（NC组）。留取SLE组患者治疗前血样，进行白细胞计数、淋巴细胞计数、免疫球蛋白、补体、C反应蛋白（CRP）、红细胞沉降率、细胞因子、T细胞亚群等项目检测。利用实时PCR检测SLE组患者CD4⁺T细胞和CD8⁺T细胞 α7nAChR mRNA的表达水平。采用Pearson相关或Spearman相关分析 α7nAChR mRNA表达与SLE患者各项临床指标的关系。结果 与NC组比较，SLE组外周血中CD4⁺T细胞和CD8⁺ T细胞 α7nAChR mRNA表达水平均显著降低（分别为1.02±0.49 vs0.50±0.27，P=0.005；1.83±0.46 vs 0.83±0.58，P=0.001）。SLE组患者外周血中CD4⁺ T细胞 α7nAChR mRNA水平与IgG（r =-0.637，P=0.035）、 CRP （r=-0.611，P=0.034）及系统性红斑狼疮疾病活动指数（SLEDAI）评分（r=-0.605，P=0.030）显著负相关。结论 SLE患者外周血中T细胞 α7nAChR mRNA低表达； α7nAChR mRNA表达与SLEDAI、血清IgG和CRP水平显著负相关，提示α7nAChR可能参与了SLE的发生发展，可作为判定SLE疾病活动的临床新指标。

系统性红斑狼疮患者外周血中T细胞α7nAChR mRNA的表达及其临床意义

王思茹, 杨娉婷

1. 中国医科大学 附属盛京医院风湿免疫科, 沈阳 110004;
2. 中国医科大学 附属第一医院风湿免疫科, 沈阳 110001

收稿日期:2022-11-21出版日期:2023-07-30发布日期:2023-07-08
通讯作者:杨娉婷E-mail:yangpingtingting@163.com
作者简介:王思茹(1994-),女,医师,硕士.
基金资助:辽宁省****滚动支持基金（28020）


关键词: 系统性红斑狼疮, T细胞, α7烟碱型乙酰胆碱受体
Abstract: Objective To investigate the expression and clinical significance of α7 nicotinic acetylcholine receptor（α7nAChR）mRNA in peripheral blood T cells of patients with systemic lupus erythematosus（SLE）. Methods We recruited 16 patients with SLE（the SLE group）who received treatment at the Department of Rheumatology and Immunology，The First Hospital of China Medical University. Additionally，we enrolled 16 healthy individuals matched for sex and age as the control group. Blood samples were collected prior to treatment. Various tests were conducted，including peripheral blood cell counting for white blood cells and lymphocytes，immunoglobulin assays， complement assays，C-reactive protein（CRP）tests，erythrocyte sedimentation tests，cytokine expression tests，and T cell subset tests. The α7nAChR mRNA expression in CD4⁺ and CD8⁺ T cells was analyzed using real time PCR in the SLE group. Pearson’s correlation or Spearman’s correlation analysis was used to explore the correlation between the clinical indicators and their corresponding α7nAChR mRNA expression in patients with SLE. Results Compared with the control group（1.02±0.49，1.83±0.46），expression of α7nAChR mRNA in CD4⁺ and CD8⁺ T cells was significantly lower in the SLE group（0.50±0.27，P=0.005；0.83±0.58，P=0.001）. Notably，in SLE patients，the level of α7nAChR mRNA in peripheral CD4⁺ T cells exhibited a significant negative correlation with serum IgG （r=-0.637，P=0.035），CRP（r=-0.611，P=0.034），and systemic lupus erythematosus disease activity index（SLEDAI） （r=-0.605， P=0.030）. Conclusion The expression of α7nAChR mRNA is low in the peripheral blood T cells of patients with SLE.α7nAChR mRNA expression was negatively correlated with SLEDAI scores，serum IgG levels，and CRP levels. These results suggest that α7nAChR may be involved in the pathogenesis and progression of SLE and could serve as a novel clinical indicator for assessing SLE disease activity.
Key words: systemic lupus erythematosus, T cell, α7 nicotinic acetylcholine receptor
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