摘要： 目的 检测miR-183-5p和NDUFA4L2在肾透明细胞癌中的表达特征，分析二者相关性及其与术后生存时间的关系。方法 收集确诊为肾透明细胞癌并行手术治疗的患者共57例，术后留取肿瘤组织和癌旁正常肾组织。应用实时荧光定量PCR （qRT-PCR）检测组织中miR-183-5p的表达，应用免疫组织化学法检测NDUFA4L2的表达。应用qRT-PCR检测人肾透明细胞癌786-0细胞株和人肾皮质近曲小管上皮HK-2细胞株中miR-183-5p和NDUFA4L2 mRNA的表达。结果 肾透明细胞癌中miR-183-5p表达量明显低于正常肾组织，NDUFA4L2阳性率明显高于正常肾组织（均P < 0.05）。miR-183-5p表达量和NDUFA4L2阳性率在不同WHO/ISUP分级、有无肾窦累犯和不同肿瘤最大径间比较，差异有统计学意义（P < 0.05）。miR-183-5p表达量和NDUFA4L2评分均与术后生存时间相关，miR-183-5p表达量和NDUFA4L2评分呈负相关。786-0细胞株中，miR-183-5p表达量明显低于HK-2细胞株，NDUFA4L2 mRNA表达量明显高于HK-2细胞株（均P < 0.05）。结论 肾透明细胞癌中miR-183-5p和NDUFA4L2异常表达且二者呈负相关，对病变的形成和进展具有促进作用，检测二者的表达可能对预后有预测价值。

miR-183-5p和NDUFA4L2在肾透明细胞癌中的表达及相关性

刘彤崴¹, 李良², 王卓², 陈静¹

1. 锦州医科大学附属第一医院肾内科, 辽宁 锦州 121000;
2. 锦州医科大学附属第三医院泌尿外科, 辽宁 锦州 121000

收稿日期:2022-09-09出版日期:2023-04-30发布日期:2023-04-15
通讯作者:李良E-mail:524137345@qq.com
作者简介:刘彤崴(1965-),女,副教授,本科.
基金资助:中国医疗手牵手工程委员会、北京医学奖励基金会项目（YXTL-2021-1092-0640）


关键词: 肾透明细胞癌, miR-183-5p, NDUFA4L2, 预后
Abstract: Objective To detect the characteristics of the expressions of microRNA-183-5p (miR-183-5p) and NDUFA4L2 in renal clear cell carcinoma and analyze their correlation and effect on postoperative survival time. Methods A total of 57 patients with renal clear cell carcinoma who underwent surgery were included, and renal cancer and adjacent normal renal tissues were collected. The expressions of miR-183-5p and NDUFA4L2 were detected by real-time fluorescence quantitative PCR (qRT-PCR) and immunohistochemistry, respectively. The expressions of miR-183-5p and NDUFA4L2 mRNA in human renal clear cell carcinoma cell line 786-0 and human renal pro-ximal tubular epithelial cell line HK-2 were detected by qRT-PCR. Results The expression level of miR-183-5p was significantly lower and the positive expression rate of NDUFA4L2 was significantly higher in renal cancer tissues than that of adjacent normal tissues (P < 0.05). The expression level of miR-183-5p and the positive expression rate of NDUFA4L2 were significantly different in various WHO/ISUP grades, renal sinus invasion, and maximum tumor diameters (P < 0.05). The expression level of miR-183-5p and NDUFA4L2 score were correlated with postoperative survival time. A negative correlation was found between expression level of miR-183-5p and NDUFA4L2 score. The expression of miR-183-5p was significantly lower and the expression of NDUFA4L2 mRNA was significantly higher in the 786-0 cell line than that of the HK-2 cell line (P < 0.05). Conclusion miR-183-5p and NDUFA4L2 are abnormally expressed and nega-tively correlated in renal clear cell carcinoma, which can promote lesion formation and progression. The expressions of miR-183-5p and NDUFA4L2 may be related to the prognosis.
Key words: renal clear cell carcinoma, miR-183-5p, NDUFA4L2, prognosis
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