摘要： 目的 探讨Toll样受体4 （TLR4）异常表达对卵巢癌细胞增殖与凋亡的影响。方法 免疫组化法检测卵巢癌及其周围正常组织中TLR4的表达情况；构建针对TLR4基因的siRNA，转染人卵巢癌细胞株SKOV3，实时定量聚合酶链反应（qRT-PCR）检测TLR4 mRNA表达，Western blotting检测TLR4蛋白表达；CCK-8法检测TLR4 siRNA转染SKOV3细胞6~24 h后细胞的生长情况，流式细胞仪检测细胞转染24 h后的凋亡情况。结果 TLR4在卵巢癌及其周围正常组织中均有表达，且卵巢癌组织中TLR4表达量明显高于癌旁组织（P<0.05）；TLR4 siRNA转染后，SKOV3细胞中TLR4 mRNA及蛋白表达水平明显降低（P<0.05）；siRNA转染24 h后，SKOV3细胞活力下降，细胞凋亡率升高（P<0.05）。结论 TLR4参与了卵巢癌细胞增殖与凋亡的调控，干扰TLR4能抑制SKOV3细胞增殖，诱导细胞凋亡。

Toll样受体4参与卵巢癌细胞增殖与凋亡的调控

郑金丹¹, 于晓萌², 张逸文¹, 王莉¹

1. 锦州医科大学附属第一医院妇产科, 辽宁 锦州 121001;
2. 锦州市妇婴医院妇产科, 辽宁 锦州 121001

收稿日期:2020-03-04出版日期:2023-03-30发布日期:2023-03-21
通讯作者:王莉E-mail:Rose130@sohu.com
作者简介:郑金丹(1987-),男,主治医师,硕士.
基金资助:辽宁省自然科学基金（20180551184）


关键词: 卵巢癌, Toll样受体4, 小干扰RNA
Abstract: Objective To investigate the effects of abnormal expression of Toll-like receptor 4 （TLR4） on the proliferation and apoptosis of ovarian cancer cells. Methods TLR4 expression in the ovarian cancer cells and surrounding normal tissues was detected by immunohistochemistry. siRNA targeting TLR4 gene was constructed and transfected into human ovarian cancer cell line SKOV3. TLR4 mRNA and protein expression levels were detected by real-time quantitative polymerase chain reaction，and Western blotting，respectively. Cell counting kit-8 method assay was used to measure viability of SKOV3 cells after transfection with TLR4 siRNA for 6-24 h，and flow cyto-metry was used to detect the apoptosis of SKOV3 cells after transfection for 24 h. Results TLR4 was expressed in both ovarian cancer cells and adjacent normal tissues；however the expression level of TLR4 in the ovarian cancer tissues was significantly higher than that in the adjacent normal tissues （P<0.05）. After siRNA transfection，the expression levels of TLR4 mRNA and protein in the SKOV3 cells were significantly decreased （P<0.05）. After 24 h siRNA transfection，SKOV3 cell viability decreased，whereas the apoptosis rate increased （P<0.05）. Conclusion TLR4 is involved in the regulation of ovarian cancer cell proliferation and apoptosis，and interference with TLR4 can inhibit SKOV3 cell proliferation and induce apoptosis.
Key words: ovarian cancer, Toll-like receptor 4, small interfering RNA
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